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Biomedical Engineering and Bioengineering Commons

Open Access. Powered by Scholars. Published by Universities.®

2013

The Summer Undergraduate Research Fellowship (SURF) Symposium

Life Sciences

MCD

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Full-Text Articles in Biomedical Engineering and Bioengineering

Bicistronic Design For Precise And Reliable Gene Expression, Nidhi N. Menon, Jenna Rickus Oct 2013

Bicistronic Design For Precise And Reliable Gene Expression, Nidhi N. Menon, Jenna Rickus

The Summer Undergraduate Research Fellowship (SURF) Symposium

Despite having progressed extensively in the field of synthetic biology in terms of DNA synthesis, analysis and transplanting, we still cannot reliably, quantitatively measure expression of new genetic constructs. We engineered a biobrick compatible expression cassette to control transcription and translation initiation which can be reused in new genetic contexts. Previous research has shown that the Bicistronic design have much lesser variations in expression with varying genes of interest as compared to the regular monocistronic design.(Mutalik, Endy, Guimaraes, Cambray, Lam, Juul, Tran & Paull, 2013) The Bicistronic design(BCD) consists of two Shine-Dalgarno sequences in its translation element which when ...