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Full-Text Articles in Biomedical Engineering and Bioengineering
Bicistronic Design For Precise And Reliable Gene Expression, Nidhi N. Menon, Jenna Rickus
Bicistronic Design For Precise And Reliable Gene Expression, Nidhi N. Menon, Jenna Rickus
The Summer Undergraduate Research Fellowship (SURF) Symposium
Despite having progressed extensively in the field of synthetic biology in terms of DNA synthesis, analysis and transplanting, we still cannot reliably, quantitatively measure expression of new genetic constructs. We engineered a biobrick compatible expression cassette to control transcription and translation initiation which can be reused in new genetic contexts. Previous research has shown that the Bicistronic design have much lesser variations in expression with varying genes of interest as compared to the regular monocistronic design.(Mutalik, Endy, Guimaraes, Cambray, Lam, Juul, Tran & Paull, 2013) The Bicistronic design(BCD) consists of two Shine-Dalgarno sequences in its translation element which when combined …
Artificial Yeast Polarization Controlled By Chemical Gradient, James K. Nolan, Bernard Tao
Artificial Yeast Polarization Controlled By Chemical Gradient, James K. Nolan, Bernard Tao
The Summer Undergraduate Research Fellowship (SURF) Symposium
Engineering synthetic multicellular systems will lead to new synthetic biology technological platforms, inform developmental biology through recapitulation of natural systems and possibly unveil novel morphologies with practical applications not before reached throughout natural history (Maharbiz, 2012). Creating an exogenous molecular circuit that will polarize unicellular cells into “apical” and “basal” domains relative to a substrate plane would fulfill a missing component towards fully multicellular synthetic cellular communities (Maharbiz, 2012). To this end, a PIP3 polarization network previously designed by Chau and associates (Chau, Walter, Gerardin, Tang, Lim 2012) was coupled to the specific activation by niacin of a recombinant …