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Biomedical Engineering and Bioengineering Commons

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2013

FIU Electronic Theses and Dissertations

Electrochemical

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Electrochemical Immunosensing Of Cortisol In An Automated Microfluidic System Towards Point-Of-Care Applications, Abhay Vasudev May 2013

Electrochemical Immunosensing Of Cortisol In An Automated Microfluidic System Towards Point-Of-Care Applications, Abhay Vasudev

FIU Electronic Theses and Dissertations

This dissertation describes the development of a label-free, electrochemical immunosensing platform integrated into a low-cost microfluidic system for the sensitive, selective and accurate detection of cortisol, a steroid hormone co-related with many physiological disorders. Abnormal levels of cortisol is indicative of conditions such as Cushing’s syndrome, Addison’s disease, adrenal insufficiencies and more recently post-traumatic stress disorder (PTSD). Electrochemical detection of immuno-complex formation is utilized for the sensitive detection of Cortisol using Anti-Cortisol antibodies immobilized on sensing electrodes. Electrochemical detection techniques such as cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) have been utilized for the characterization and sensing ...