Biomedical Engineering and Bioengineering Commons^™

Slowing The Onset Of Hypoxia Increases Colony Forming Efficiency Of Connective Tissue Progenitor Cells In Vitro, Christopher M. Heylman, Tonya N. Caralla, Cynthia A. Boehm, Thomas E. Patterson, George F. Muschler

Biomedical Engineering

Background: Survival and colony formation by transplanted tissue derived connective tissue progenitor cells (CTPs) are thought to be important factors in the success of clinical tissue engineering strategies for bone regeneration. Transplantation of cells into defects larger than a few millimeters expose cells to a profoundly hypoxic environment. This study tested the hypothesis that delaying the onset of hypoxia will improve the survival and performance of CTPs in vitro.

Methods: To mimic declines seen in an avascular in vivo bone defect, colony forming efficiency by marrow derived nucleated cells was assessed under osteogenic conditions. Variation in the rate of …