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Biomedical Engineering and Bioengineering Commons

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Articles 1 - 6 of 6

Full-Text Articles in Biomedical Engineering and Bioengineering

Advances In Homology Directed Genetic Engineering Of Human Pluripotent And Adult Stem Cells, Kalpith Ramamoorthi, Donald Curtis, Prashanth Asuri Oct 2013

Advances In Homology Directed Genetic Engineering Of Human Pluripotent And Adult Stem Cells, Kalpith Ramamoorthi, Donald Curtis, Prashanth Asuri

Bioengineering

The ability to introduce precise genomic modifications in human cells has profound implications for both basic and applied research in stem cells, ranging from identification of genes regulating stem cell self-renewal and multilineage differentiation to therapeutic gene correction and creation of in vitro models of human diseases. However, the overall efficiency of this process is challenged by several factors including inefficient gene delivery into stem cells and low rates of homology directed site-specific targeting. Recent studies report the development of novel techniques to improve gene targeting efficiencies in human stem cells; these methods include molecular engineering of viral vectors o ...


The Trm2h : Detecting Protein-Protein Interactions By Engineering The Cell As A Biosensor, Elyse Shimomura, Paulina Perezalonso Aug 2013

The Trm2h : Detecting Protein-Protein Interactions By Engineering The Cell As A Biosensor, Elyse Shimomura, Paulina Perezalonso

Bioengineering Senior Theses

Protein-protein interactions regulate key cellular functions and cell signaling pathways in the body; biological systems can be disrupted by protein interactions or lack of interactions that cause cell dysfunction and can lead to disease or illness. However, studying these interactions is complex and difficult to quantify. In this project, we examine the interaction of two putative cancer biomarkers, Bax and PDCDS, using the tetracycline repressor-based mammalian two-hybrid system (trM2H). This in vivo system engineers the cell as a biosensor using methods of DNA cloning and mammalian cell transfections that easily detects and quantities the interaction of two proteins by the ...


Analyzing Surface Protein Expression And Internalization, William Truong, Josergio Zaragoza Jul 2013

Analyzing Surface Protein Expression And Internalization, William Truong, Josergio Zaragoza

Bioengineering Senior Theses

G-protein coupled receptors (GPCRs) are currently the largest class of membrane receptors and are targeted by a majority of the modern drug therapeutics. In addition, they partake in many physiological and pathological processes such as inflammation, growth, and hormone responses. Most importantly, GPCRs are targets of many disease-specific pathways such as Alzheimers, hypertension, leukemia, and depression. As a result, there is an immense interest in studying GPCRs as this area provides further knowledge into the specific disease pathways and allows the discovery of novel therapeutics. In order to have a better understanding of pathways, scientists have studied GPCR activation. The ...


Amperometric Detection Of Bioamines In Cancer Borealis Using Microchip Capillary Electrophoresis Integrated With Micellar Chromatography, Ajay Fernandez, Jason Howard, Christina Shuh Jun 2013

Amperometric Detection Of Bioamines In Cancer Borealis Using Microchip Capillary Electrophoresis Integrated With Micellar Chromatography, Ajay Fernandez, Jason Howard, Christina Shuh

Bioengineering Senior Theses

In order to study neuromodulation in Cancer borealis, commonly known as the Jonah crab, we designed a microchip capillary electrophoretic (CE) device with micellar electrokinetic chromatography (MEKC) to separate bioamines and detect their concentrations using amperometric detection (AD). The analysis will be used to characterize the bioamines: dopamine, octopamine, tyramine, serotonin, norepinephrine, and gammaaminobutyric acid (GABA). The device measures the concentration of bioamines in microdialysate samples taken directly from the pericardial cavity of the crab. The research on Jonah crabs will be applied to enhance our understanding of the crab neurology. Since the structure and in some instances, the function ...


Microfluidic Detection Of Arsenic Contamination In Groundwater, Mary Reynolds, Kyle Perricone Jun 2013

Microfluidic Detection Of Arsenic Contamination In Groundwater, Mary Reynolds, Kyle Perricone

Bioengineering Senior Theses

The difficulty of detecting small quantities of arsenic in water currently threatens the health of millions of people worldwide, as long-term exposure to arsenic has been associated with both cancerous and noncancerous health risks. Existing technologies make it possible to very accurately quantify arsenic levels in water; however the expense, extensive training, and off-site analysis required by these methods impede wide scale use. Here, we report on research to develop an affordable and point-of-use microfluidic platform capable of detecting trace amounts of arsenic in groundwater samples. We intend this device to meet the World Health Organization's (WHO's) ASSURED ...


Uniflow: A Collapsible Prostatic Stent To Treat The Symptoms Of Benign Prostatic Hyperplasia, Brett Simons, Senn Foote, Doug Yoon Jun 2013

Uniflow: A Collapsible Prostatic Stent To Treat The Symptoms Of Benign Prostatic Hyperplasia, Brett Simons, Senn Foote, Doug Yoon

Bioengineering Senior Theses

BPH is a widespread problem that currently does not have any highly effective and cheap solutions. We propose a solution involving a collapsible and durable stent for insertion in to the urethra to aid in urination by opening the urethra only when needed.