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Biomedical Engineering and Bioengineering Commons

Open Access. Powered by Scholars. Published by Universities.®

2013

None

Cong T Trinh

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Full-Text Articles in Biomedical Engineering and Bioengineering

Smet: Systematic Multiple Enzyme Targeting–A Method To Rationally Design Optimal Strains For Target Chemical Overproduction, David Flowers, Adam Thompson, Douglas Birdwell, Tsewei Wang, Cong Trinh Dec 2012

Smet: Systematic Multiple Enzyme Targeting–A Method To Rationally Design Optimal Strains For Target Chemical Overproduction, David Flowers, Adam Thompson, Douglas Birdwell, Tsewei Wang, Cong Trinh

Cong T Trinh

Identifying multiple enzyme targets for metabolic engineering is very critical for redirecting cellular metabolism to achieve desirable phenotypes, e.g., overproduction of a target chemical. The challenge is to determine which enzymes and how much of these enzymes should be manipulated by adding, deleting, under-, and/or over-expressing associated genes. In this study, we report the development of a systematic multiple enzyme targeting method (SMET), to rationally design optimal strains for target chemical overproduction. The SMET method combines both elementary mode analysis and ensemble metabolic modeling to derive SMET metrics including l-values and c-values that can identify rate-limiting reaction steps ...