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Full-Text Articles in Biomedical Engineering and Bioengineering
Sintered Carbonate Apatites As Bone Substitutes, Yutaka Doi
Sintered Carbonate Apatites As Bone Substitutes, Yutaka Doi
Cells and Materials
Sintering of carbonate apatites was investigated and the usefulness of sintered specimens as bone substitutes was evaluated in vitro and in vivo. Osteoclasts appeared to be capable of resorbing sintered carbonate apatite, which was as soluble as deproteinated bone and much more soluble than sintered hydroxyapatite in weak acids. In skull defects of Wistar rats, sintered carbonate apatite particles resorbed to an appreciable extent, but the rate of resorption did not exceed that of new bone formation. At 4 weeks after implantation the defects were filled almost completely with new bone that compared favorably with the host bone. These …
Osteoclast Differentiation In Cocultures Of Chondrogenic Cell Line Rcj 3.1c5.18 And Mouse Or Rat Bone Marrow: Dependence On Culture Substrate And Association With Alkaline Phosphatase Positive Marrow Stromal Cells, G. I. Anderson, J. N. M. Heersche
Osteoclast Differentiation In Cocultures Of Chondrogenic Cell Line Rcj 3.1c5.18 And Mouse Or Rat Bone Marrow: Dependence On Culture Substrate And Association With Alkaline Phosphatase Positive Marrow Stromal Cells, G. I. Anderson, J. N. M. Heersche
Cells and Materials
We investigated the formation of tartrate-resistant acid phosphatase positive (TRAP+) colonies and multinucleated cells (MNCs) in rat and mouse marrow cultures alone and cocultured with the chondrogenic cell line, RCJ3.1C5.18 on different substrata. In mouse marrow cultured in 35 mm dishes, few TRAP+ MNCs developed, while in rat marrow cultures, many TRAP+ MNCs, which possessed calcitonin receptors and resorbed bone, developed. In both rat and mouse cultures, TRAP+ MNC first appeared at Day 4 and grew in number up to Day 8. When both marrows were cocultured with RC3.1C5.18 cells, TRAP+ colony numbers increased relative to marrow alone. In marrow …