Biomedical Engineering and Bioengineering Commons^™

Development Of Collagen-Based Scaffolds For Differentiation Of Induced Pluripotent Stem Cells, Siteng Fang

Graduate Dissertations and Theses

Collagen hydrogel has been broadly studied and applied in engineering 3D scaffold materials in tissue engineering. A collagen hydrogel can provide cells with a porous and soft environment to proliferate and differentiate. However, lacking mechanical stiffness and shrinkage resistance made it a challenge to sustain shape and size during a long stem cell differentiation process. In addition, a cytocompatible scaffold for human induced pluripotent stem cell (iPSC)-laden culture has not been fully investigated. The goal of this study is to develop stable and biocompatible collagen-based scaffolds that are suitable for direct seeding and lineage progression of iPSCs. In this work, …