Biomedical Engineering and Bioengineering Commons^™

Unlocking The Secrets Of Multi-Flagellated Propulsion, Stefan Oma Nwandu-Vincent

Doctoral Dissertations

In this work, unique high-speed imaging platforms and an array of theoretical analysis methods are used to thoroughly investigate eukaryotic multi-flagellated propulsion using Tritrichomonas foetus as a test case. Through experimental observations through our imaging system with superior resolution and capture rate exceeding that of previous studies, it was discovered for the first time that the T. foetus employs a strategy similar to that of the “run and tumble” strategies found in bacteria and Chlamydomonas; it has two distinct flagellar beating patterns that result in two different body swimming motions, linear and turning swimming.

These two flagella patterns were …

Development, Characterization, And Optimization Of Process For Minimal Functional Barrier And Channel Width In Nitrocellulose Paper Microfluidic Diagnostic Platforms, Ryan Daniel Silva

Biomedical Engineering

A central goal of diagnostic microfluidics is to reduce the cost of diagnostic medicine by reducing the equipment and reagents needed to perform diagnostic tests. The literature has demonstrated that a wax printer can be used to pattern nitrocellulose paper with hydrophobic barriers to direct a sample in a defined reaction path, eliminating the need for external pumps and controllers. However, manufacturing methods for minimizing sample volume (and thus reagent volume) in a paper-diagnostic chip have not been well defined. In this work, we experimentally determine manufacturing processes for creating functional features of minimal size—effectively reducing the sample size and …

Effect Of Junction Geometry On Monodispersed Microdroplet Generation In Microfluidic Aqueous Two-Phase Systems, Young Gyu Nam

Theses and Dissertations

Aqueous two-phase system (ATPS) consists of two immiscible water-based solutions of polymers, which can form phase partitioning. Dextran and polyethylene glycol I used in this thesis is the one of common components of aqueous two-phase system give a reliable and incompatible environment for purification of biomedical products and cellular macromolecules. Recently, ATPS have received increasing attention as a separation method in microfluidic device due to the advantages of biocompatibility, unlimited combination, and low interfacial tension. Hence, it became an important to discover researches related to ATPS microfluidic device.

Microdroplets produced in microfluidic device are a largely interesting phenomenon for various …

Thermoelectric Elisa For Quantification Of 8ohdg In A Microfluidic Device, Gergana Nestorova

Doctoral Dissertations

This research demonstrates the feasibility of a novel method for performing thermoelectric enzyme-linked immunosorbent assay (ELISA) in a microfluidic device. The feasibility of the thermoelectric ELISA is demonstrated by measuring the concentration of 8-hydroxy 2-deoxyguanosine (8OHdG) in urine samples from amyloid precursor protein (APP) transgenic mice. The detection method is based on formation of a complex between 8OHdG and anti-8OHdG capture antibody conjugated to biotin. The complex is immobilized over the measuring junctions of a thermopile via biotin streptavidin interaction. The concentration of the analyte is determined by using enzyme linked secondary IgG antibody specific to the primary one. The …

Utilizing Dielectrophoresis To Determine The Physiological Differences Of Eukaryotic Cells, Tayloria Nicole Gail Adams

Dissertations, Master's Theses and Master's Reports - Open

Type 1 diabetes affects over 108,000 children, and this number is steadily increasing. Current insulin therapies help manage the disease but are not a cure. Over a child’s lifetime they can develop kidney disease, blindness, cardiovascular disease and many other issues due to the complications of type 1 diabetes. This autoimmune disease destroys beta cells located in the pancreas, which are used to regulate glucose levels in the body. Because there is no cure and many children are affected by the disease there is a need for alternative therapeutic options that can lead to a cure.

Human mesenchymal stem cells …