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Biomedical Engineering and Bioengineering Commons

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Full-Text Articles in Biomedical Engineering and Bioengineering

Mechanical And Physical Properties Of Spider Silk Films Made From Organic And Water-Based Dopes, Chauncey Lewis Tucker May 2014

Mechanical And Physical Properties Of Spider Silk Films Made From Organic And Water-Based Dopes, Chauncey Lewis Tucker

All Graduate Theses and Dissertations

In this project, we focus on developing a method to produce synthetic spider silk thin films. Using these films we optimized mechanical properties, lowered cost, and improved the environmental impact using different processing methods. Applications for spider silk films are broad, ranging from physical protection to biocompatible materials. This project was designed to improve mechanical properties and production methods of films made from synthetic forms of MaSp1 and MaSp2 from the dragline silk of Nephila clavipes. We have increased the mechanical stress (200 MPa) to more than 4 times that of similar products with elongations as high as 35%. The ...


Optimization Of Wastewater Microalgae Pretreatment For Acetone, Butanol, And Ethanol Fermentation, Yessica A. Castro May 2014

Optimization Of Wastewater Microalgae Pretreatment For Acetone, Butanol, And Ethanol Fermentation, Yessica A. Castro

All Graduate Theses and Dissertations

Acetone-butanol-ethanol (ABE) fermentation from wastewater microalgae by Clostridium saccharoperbutylacetonicum N1-4 is a novel bioprocess that utilizes waste substrate to generate valuable solvents. Butanol, the most abundant product resulting from ABE fermentation, is an environmentally safe and high performing fuel that can be utilized as a drop-in-fuel; however, high operational costs and low ABE yield present challenge in scale-up of the process. The utilization of algae as a substrate requires pretreatment prior to fermentation to increase the bioavailability of the algal fermentable sugars and to improve the conditions of the pre-fermentation medium. The purpose of this thesis was to optimize wastewater ...


Effect Of Poultry Litter Biochar On Saccharomyces Cerevisiae Growth And Ethanol Production From Steam-Exploded Poplar And Corn Stover, Oumou Diallo May 2014

Effect Of Poultry Litter Biochar On Saccharomyces Cerevisiae Growth And Ethanol Production From Steam-Exploded Poplar And Corn Stover, Oumou Diallo

All Graduate Theses and Dissertations

The use of ethanol produced from lignocellulosic biomass for transportation fuel offers solutions in reducing environmental emission and the use of non-renewable fuels. However, lignocellulosic ethanol production is still hampered by economic and technical obstacles. For instance, the inhibitory effect of toxic compounds produced during biomass pretreatment was reported to inhibit the fermenting microorganisms, hence there was a decrease in ethanol yield and productivity. Thus, there is a need to improve the bioconversion of lignocellulosic biomass to ethanol in order to promote its commercialization. The research reported here investigated the use of poultry litter biochar to improve the ethanol production ...


Sustainable Production Of Novel Biomaterials In Escherichia Coli, Asif Rahman May 2014

Sustainable Production Of Novel Biomaterials In Escherichia Coli, Asif Rahman

All Graduate Theses and Dissertations

The biotechnology revenues in the United States exceeded $100 billion in 2010 and the potential impact of synthetic biological engineering has been identified nationally as an emerging technology to further expand the national bioeconomy. Synthetic biological engineering approaches biology from an engineering perspective to make biology easier to engineer. The potential to engineer microorganisms for novel applications can have far-reaching implications and benefits for society. Some of the potential applications range from biosensors, biofuels, therapeutics, and biomaterials.

In this study two biomaterials were produced in genetically engineered Escherichia coli: polyhydroxybutyrates (PHBs) and spider silk. PHBs are bioplastics that have similar ...


Alkaloid Production By Hairy Root Cultures, Bo Zhao May 2014

Alkaloid Production By Hairy Root Cultures, Bo Zhao

All Graduate Theses and Dissertations

In the present research, nicotine alkaloid production by Nicotiana tabacum (tobacco) hairy roots and tropane alkaloid production by Hyoscyamus niger hairy roots were investigated. The first objective of this research was to improve the oxygen mass transfer in hairy root cultures with microbubbles. Oxygen was shown as a critical nutrient for the growth of tobacco and H. niger hairy roots. In a 1-liter fermentor, microbubble dispersion improved the oxygen mass transfer, tobacco hairy root growth, and nicotine production in the medium. In a novel ground-joint column bioreactor, microbubbles enhanced the oxygen mass transfer and the growth of H. niger hairy ...


Distribution Of Heavy Metals From Flue Gas In Algal Bioreactor, Katerine Napan May 2014

Distribution Of Heavy Metals From Flue Gas In Algal Bioreactor, Katerine Napan

All Graduate Theses and Dissertations

Algae are microscopic organisms with a great potential to produce biomass and lipids at productivities several times higher than terrestrial crops. To grow, these organisms consume carbon dioxide (CO2), a greenhouse gas. This gas, emitted primarily by power plants after coal burning, can be effectively used for algae production, thus resulting in CO2 remediation and biomass beneficial utilization as feedstuff, industrial filler and biodiesel feedstock. However, since coal is a fuel mined from the earth’s crust, it contains heavy metals that are released during coal burning and inevitably enter the algal cultivation system, contaminating the water were algae is ...


Excimer-Monomer Switching Molecular Beacon: The Study On Synthetic Cryptosporidum Dna Detection, Thermodynamics, And Magnesium Effects, Michael L. Davis May 2014

Excimer-Monomer Switching Molecular Beacon: The Study On Synthetic Cryptosporidum Dna Detection, Thermodynamics, And Magnesium Effects, Michael L. Davis

All Graduate Theses and Dissertations

Cryptosporidium parvum is a deadly waterborne protozoan parasite that invades the gastrointestinal tract of humans and causes severe to life-threatening gastro enteric disease. Due to the ubiquitous nature of Cryptosporidium parvum in the world’s water, it is necessary to determine the source of an outbreak. Rapid detection and identification of various genotypes of Cryptosporidium are a valuable goal in determining the source of the pathogen in a human epidemic. Exploitation of gene sequences specific to species is a powerful tool detecting pathogens.

Molecular beacons are one of these tools for high selectivity and specificity detection of DNA and RNA ...


Metabolic Modeling Of Spider Silk Production In E. Coli, Sarah Allred May 2014

Metabolic Modeling Of Spider Silk Production In E. Coli, Sarah Allred

All Graduate Theses and Dissertations

Spider silk has the potential to be a useful biomaterial due to its high tensile strength and elasticity. It is also biocompatible and biodegradable, making it useful for wound dressings and sutures, tissue and bone scaffolds, vessels for drug delivery, and ligament and tendon replacements. In some studies where spider silk has been used to grow cells, the silk has promoted more cell growth than the control. However, it is difficult to obtain the high volume of silk needed for these undertakings on a large scale. Spiders are territorial and cannibalistic, so they cannot be easily farmed. Therefore, spider silk ...