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Full-Text Articles in Biomedical Engineering and Bioengineering
Development Of A Protocol To Measure Gene Expression In The Mouse Tibia, Daniel Hoover
Development Of A Protocol To Measure Gene Expression In The Mouse Tibia, Daniel Hoover
Biomedical Engineering
Numerous molecular factors active in bone tissue direct fracture repair and remodeling which can be altered by disease conditions such as Peripheral Arterial Disease (PAD) and Osteoporosis. Methods of molecular biology are commonly applied to investigate the expression and role of these molecular factors. This project presents a robust three-step protocol for examining gene expression in the mouse tibia. The protocol begins with isolating RNA from a flash frozen tibia sample. The isolated RNA is reverse transcribed into cDNA. Finally, PCR is performed to indentify expressed genes. Establishing this protocol will allow further research into the mechanisms of bone remodeling …
Mechanical Characterization Of An External Fixator For Use In A Mouse Model, Thomas Albano
Mechanical Characterization Of An External Fixator For Use In A Mouse Model, Thomas Albano
Honors Theses
Understanding the process of bone healing has become a fundamental part of medical research due to the approximately one million fractures which occur annually in the United States. The current methods of fracture fixation which use intramedullary rods, external fixators, and fracture plates are effective but not ideal. These fracture fixation methods can lead to mal-union or non-union due to improper callus formation stemming from inadequate fixation and support. When mal-union and non-union occur, the structural integrity of the bone becomes greatly sacrificed and the patient is left to deal with continual pain. Previous studies have suggested that the mechanical …