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Full-Text Articles in Biomedical Engineering and Bioengineering
Vascular Endothelial Dysfunction And Effects On Arterial Wall Microstructure, Jeffrey Thomas Rodgers
Vascular Endothelial Dysfunction And Effects On Arterial Wall Microstructure, Jeffrey Thomas Rodgers
Theses and Dissertations
Vascular endothelial cell dysfunction (ECD) is a disease characterized by the inability of the vascular endothelial cells to transmit signaling molecules, namely nitric oxide (NO), in response to changes in blood flow rate. This disease is shown to be incident in the onset of more severe vascular disease such as hypertension, atherosclerosis, and heart failure. Long-term ECD is shown to cause long-term remodeling such as decreased flow-mediated dilation, increased pulse-wave velocity, intimal-medial thickening, and increased wall stiffness due to collagen deposition. We used second harmonic generation (SHG) microscopy to analyze this change in the collagen microstructure of the arterial extracellular …
Three-Dimensional Collagen Tubes For In Vitro Modeling, Rebecca Jones
Three-Dimensional Collagen Tubes For In Vitro Modeling, Rebecca Jones
Theses and Dissertations
Collagen type I represents a novel material for three-dimensional in vitro models. While two-dimensional models are typically inadequate for recreating the complex processes of the body, collagen provides a three-dimensional basis with a variety of applications, including remodeling of vascular cells under tension and vascular stenosis. Smooth muscle cells reorganize and reconstruct their environment differently under conditions of tensions, such as with sutures, or under conditions without applied external tension. Vascular stenosis, the abnormal narrowing of blood vessels, arises from defective developmental processes or atherosclerosis-related adult pathologies. Stenosis triggers a series of adaptive cellular responses that induces adverse remodeling, which …
Automated Image Analysis And Spatial Computational Modeling Of Nf-Kb In Cerebrovascular Endothelial Cells, Kasey Catalfomo
Automated Image Analysis And Spatial Computational Modeling Of Nf-Kb In Cerebrovascular Endothelial Cells, Kasey Catalfomo
Theses and Dissertations
Cerebrovascular endothelial cells play a key part in the inflammatory response of the blood-brain barrier in pathological conditions such as Alzheimer’s disease. Specifically, the NF-κB signaling pathway plays a central role. Better understanding of the factors in inflammatory disease progression can lead to more effective treatments for such devastating illnesses like Alzheimer’s, asthma, arthritis, cancer, diabetes and many more inflammatory diseases. The proposed approach analyzes spatial NF-κB distribution contained in multispectral stacked micrograph images of cerebrovascular endothelial cells indexed based on dose of the activating protein and the length of activation. Image analysis code identifies the location of nuclear boundaries …