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University of Pennsylvania

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Full-Text Articles in Biomedical Engineering and Bioengineering

Computer-Aided, Multi-Modal, And Compression Diffuse Optical Studies Of Breast Tissue, David R. Busch Jr Dec 2011

Computer-Aided, Multi-Modal, And Compression Diffuse Optical Studies Of Breast Tissue, David R. Busch Jr

Publicly Accessible Penn Dissertations

Diffuse Optical Tomography and Spectroscopy permit measurement of important physiological parameters non-invasively through ~10 cm of tissue. I have applied these techniques in measurements of human breast and breast cancer. My thesis integrates three loosely connected themes in this context: multi-modal breast cancer imaging, automated data analysis of breast cancer images, and microvascular hemodynamics of breast under compression. As per the first theme, I describe construction, testing, and the initial clinical usage of two generations of imaging systems for simultaneous diffuse optical and magnetic resonance imaging. The second project develops a statistical analysis of optical breast data from many spatial ...


Enhancing Meniscus Repair Through Biomaterial Design, Lara Ionescu Silverman Dec 2011

Enhancing Meniscus Repair Through Biomaterial Design, Lara Ionescu Silverman

Publicly Accessible Penn Dissertations

The knee meniscus is prone to damage, which leads to pain and inhibits mobility in the joint long term. Due to the minimal vascularity, low cellularity and large mechanical forces imparted on the meniscus with normal use, endogenous repair is limited. Resection of the damaged region of the tissue (meniscectomy) remains the most common treatment for a torn meniscus, but this procedure results in cartilage degradation and other adverse changes in the knee joint. Given the prevalence of meniscus damage, there is thus a pressing need for novel approaches to meniscus repair. To address this issue, this thesis developed in ...


Optimization And Translation Of Msc-Based Hyaluronic Acid Hydrogels For Cartilage Repair, Isaac E. Erickson Dec 2011

Optimization And Translation Of Msc-Based Hyaluronic Acid Hydrogels For Cartilage Repair, Isaac E. Erickson

Publicly Accessible Penn Dissertations

Traumatic injury and disease disrupt the ability of cartilage to carry joint stresses and, without an innate regenerative response, often lead to degenerative changes towards the premature development of osteoarthritis. Surgical interventions have yet to restore long-term mechanical function. Towards this end, tissue engineering has been explored for the de novo formation of engineered cartilage as a biologic approach to cartilage repair. Research utilizing autologous chondrocytes has been promising, but clinical limitations in their yield have motivated research into the potential of mesenchymal stem cells (MSCs) as an alternative cell source. MSCs are multipotent cells that can differentiate towards a ...


Cerebral Hemodynamics In High-Risk Neonates Probed By Diffuse Optical Spectroscopies, Erin M. Buckley Aug 2011

Cerebral Hemodynamics In High-Risk Neonates Probed By Diffuse Optical Spectroscopies, Erin M. Buckley

Publicly Accessible Penn Dissertations

Advances in medical and surgical care of the critically ill neonates have decreased
mortality, yet a significant number of these neonates suffer from neurodevelopmental
delays and failure in school. Thus, clinicians are now focusing on prevention of
neurologic injury and improvement of neurocognitive outcome in these high-risk infants. Assessment of cerebral oxygenation, cerebral blood volume, and the regulation of cerebral blood flow (CBF) during the neonatal period is vital for evaluating brain health. Traditional CBF imaging methods fail, however, for both ethical and logistical reasons. In this dissertation, I demonstrate the use of non-invasive optical modalities, i.e., diffuse optical ...


Chemical And Biological Strategies For Improving The Sensitivity Of Spio-Enhanced Mr Imaging, Andrew Elias Aug 2011

Chemical And Biological Strategies For Improving The Sensitivity Of Spio-Enhanced Mr Imaging, Andrew Elias

Publicly Accessible Penn Dissertations

The prevalence of cancer has seen significant growth over the last three decades, with the American Cancer Society now reporting that one in every three Americans will be afflicted with some form of cancer in their lifetime. The debilitating effects of cancer permeate beyond obvious biological ramifications, often adversely affecting patients on emotional and financial levels as well. While significant progress has been made in diagnosing and treating cancer, advancements are still needed in detecting and treating cancers at earlier stages, where clinical outcomes will be more favorable. The field of cellular and molecular imaging has emerged as a cutting-edge ...


Multiscale Modeling Of The Erbb Receptor Tyrosine Kinase Signaling Network Through Theory And Experiment, Shannon E. Telesco Aug 2011

Multiscale Modeling Of The Erbb Receptor Tyrosine Kinase Signaling Network Through Theory And Experiment, Shannon E. Telesco

Publicly Accessible Penn Dissertations

The biochemical processes occurring within a living cell span a spectrum of scales in space and time, ranging from the nano- to the macro-scale. We note that a single cellular process often operates on multiple spatial and temporal scales, and thus it becomes necessary to combine modeling techniques in multiscale approaches, in which different levels of theory are synergized to describe a system at a number of scales or resolutions. In this work we apply a multiscale modeling framework to investigate the molecular regulatory mechanisms governing the activation of the ErbB receptor tyrosine kinases, a family of kinases which are ...


An An Vitro And In Silico Investigation Of The Role Of Nmda Receptor Subtypes Following Mechanical Injury, Pallab Singh Aug 2011

An An Vitro And In Silico Investigation Of The Role Of Nmda Receptor Subtypes Following Mechanical Injury, Pallab Singh

Publicly Accessible Penn Dissertations

The N-methyl D-aspartate receptor (NMDAR), a common glutamate receptor found throughout the brain, has long been implicated as the major mediator of the pathology seen after traumatic brain injury (TBI). However, given their critical role in physiologic function of neural networks, complete inhibition of these receptors is an unsuitable therapeutic strategy. Thus, further investigation into how these receptors respond to injury is required to identify more directed therapeutic targets. Here, we aimed to use two unique experimental models to further investigate the role of NMDARs in the neuronal response to TBI, with specific emphasis on the contribution of different NMDAR ...


Quantitative Mapping Of Lung Ventilation Using Hyperpolarized Gas Magnetic Resonance Imaging, Kiarash Emami May 2011

Quantitative Mapping Of Lung Ventilation Using Hyperpolarized Gas Magnetic Resonance Imaging, Kiarash Emami

Publicly Accessible Penn Dissertations

The main objective of this project was to develop and implement techniques for high-resolution quantitative imaging of ventilation in lungs using hyperpolarized gas magnetic resonance imaging (MRI). Pulmonary ventilation is an important aspect of lung function and is frequently compromised through several different mechanisms and at varying degrees in presence of certain lung conditions, such as chronic obstructive pulmonary diseases. The primary focus of this development is on large mammalian species as a steppingstone towards translation to human subjects. The key deliverables of this project are a device for real-time mixing and delivery of hyperpolarized gases such as 3He ...


Indirect Detection Of Axonal Architecture With Q-Space Imaging, Henry H. Ong May 2011

Indirect Detection Of Axonal Architecture With Q-Space Imaging, Henry H. Ong

Publicly Accessible Penn Dissertations

Evaluating axon morphology would provide insights into connectivity, maturation, and disease pathology. Conventional diffusion MRI can provide metrics that are related to axon morphology, but cannot measure specific parameters such as mean axon diameter (MAD) and intracellular fraction (ICF). Q-space imaging (QSI) is an advanced diffusion MRI technique that may be able to provide more information on axon morphology. However, QSI has several limitations that affect its implementation and accuracy.

The main objective of this dissertation was to address these limitations and to evaluate the potential of QSI to accurately assess axon morphology. First, a custom-built high-amplitude gradient coil was ...


Unveiling The Molecular Mechanisms Regulating The Activation Of The Erbb Family Receptors At Atomic Resolution Through Molecular Modeling And Simulations, Andrew Shih May 2011

Unveiling The Molecular Mechanisms Regulating The Activation Of The Erbb Family Receptors At Atomic Resolution Through Molecular Modeling And Simulations, Andrew Shih

Publicly Accessible Penn Dissertations

The EGFR/ErbB/HER family of kinases contains four homologous receptor tyrosine kinases that are important regulatory elements in key signaling pathways. To elucidate the atomistic mechanisms of dimerization-dependent activation in the ErbB family, we have performed molecular dynamics simulations of the intracellular kinase domains of the four members of the ErbB family (those with known kinase activity), namely EGFR, ErbB2 (HER2) and ErbB4 (HER4) as well as ErbB3 (HER3), an assumed pseudokinase, in different molecular contexts: monomer vs. dimer, wildtype vs. mutant. Using bioinformatics and fluctuation analyses of the molecular dynamics trajectories, we relate sequence similarities to correspondence of ...


Regulation Of Tgf-Β1-Induced Apoptosis And Epithelial-Mesenchymal Transition By Matrix Rigidity, Jennifer L. Leight May 2011

Regulation Of Tgf-Β1-Induced Apoptosis And Epithelial-Mesenchymal Transition By Matrix Rigidity, Jennifer L. Leight

Publicly Accessible Penn Dissertations

Transforming growth factor-β (TGF-β) plays an important role in several diseases that characteristically involve changes in tissue rigidity, such as cancer and tissue fibrosis. To determine whether matrix rigidity regulates the effects of TGF-β, we examined NMuMG and MDCK epithelial cells cultured on polyacrylamide gels with varying rigidity and treated with TGF-β1. Decreasing matrix rigidity reduced cell spreading and increased TGF-β1-induced apoptosis, while increasing matrix rigidity resulted in epithelial-mesenchymal transition (EMT). To more carefully control cell spreading, microcontact printing was used to restrict ECM area and revealed that reducing cell spreading also increased apoptosis. Apoptosis on compliant substrates was associated ...


Mapping Trabecular Bone Fabric Tensor By In Vivo Magnetic Resonance Imaging, Michael J. Wald Dec 2010

Mapping Trabecular Bone Fabric Tensor By In Vivo Magnetic Resonance Imaging, Michael J. Wald

Publicly Accessible Penn Dissertations

The mechanical competence of bone depends upon its quantity, structural arrangement, and chemical composition. Assessment of these factors is important for the evaluation of bone integrity, particularly as the skeleton remodels according to external (e.g. mechanical loading) and internal (e.g. hormonal changes) stimuli. Micro magnetic resonance imaging (µMRI) has emerged as a non-invasive and non-ionizing method well-suited for the repeated measurements necessary for monitoring changes in bone integrity. However, in vivo image-based directional dependence of trabecular bone (TB) has not been linked to mechanical competence or fracture risk despite the existence of convincing ex vivo evidence. The objective ...


Computational Modeling Of Protein Kinases: Molecular Basis For Inhibition And Catalysis, Yingting Liu Dec 2010

Computational Modeling Of Protein Kinases: Molecular Basis For Inhibition And Catalysis, Yingting Liu

Publicly Accessible Penn Dissertations

Protein kinases catalyze protein phosphorylation reactions, i.e. the transfer of the γ-phosphoryl group of ATP to tyrosine, serine and threonine residues of protein substrates. This phosphorylation plays an important role in regulating various cellular processes. Deregulation of many kinases is directly linked to cancer development and the protein kinase family is one of the most important targets in current cancer therapy regimens. This relevance to disease has stimulated intensive efforts in the biomedical research community to understand their catalytic mechanisms, discern their cellular functions, and discover inhibitors. With the advantage of being able to simultaneously define structural as well ...


The Development, Characterization And Implementation Of A Reactive Oxygen Species-Responsive Ratiometric Bioluminescent Reporter And Its Use As A Sensor For Programmed Cell Death, Julie Czupryna Dec 2010

The Development, Characterization And Implementation Of A Reactive Oxygen Species-Responsive Ratiometric Bioluminescent Reporter And Its Use As A Sensor For Programmed Cell Death, Julie Czupryna

Publicly Accessible Penn Dissertations

The process of programmed cell death (PCD) is a prevalent area of scientific research and is characterized by a highly complex proteolytic cascade. PCD is involved in development, homeostasis and the immune response. Further, unregulated PCD has been implicated in various, often, devastating pathologies including cancer, autoimmune diseases and neurodegenerative disorders. The importance of PCD in human health and disease has led to the widespread utilization of genetically encoded reporters for the non-invasive imaging of PCD in vitro and in vivo; however, it is currently not well understood whether the reporters themselves are susceptible to inactivation and/or degradation during ...


Rac1-Mediated Actin Cytoskeleton Remodeling And Monolayer Barrier Properties Of Stretched Alveolar Epithelial Cells, Brian Christopher Dipaolo Dec 2010

Rac1-Mediated Actin Cytoskeleton Remodeling And Monolayer Barrier Properties Of Stretched Alveolar Epithelial Cells, Brian Christopher Dipaolo

Publicly Accessible Penn Dissertations

Mechanical ventilation is vital for supporting patients with pulmonary insufficiency. However, experimental data suggests that pulmonary injury can occur during mechanical ventilation as a result of elevated regional lung volumes resulting in excessive alveolar epithelial cell (AEC) stretch due to basement membrane deformation and reduced barrier function.

AECs maintain integrity of the blood-gas barrier with gasket like intercellular tight junctions (TJ) which are anchored internally to the actin cytoskeleton. Our goal was to evaluate the effect of physiologically relevant, high magnitude biaxial stretch on the actin cytoskeleton, a principal regulator of monolayer barrier properties, as a foundation to develop strategies ...


Integrating Electrophysiological, Mechanical, And Optical Methods To Define The Mechanisms Of Painful Facet Joint Injury, Kyle P. Quinn Dec 2010

Integrating Electrophysiological, Mechanical, And Optical Methods To Define The Mechanisms Of Painful Facet Joint Injury, Kyle P. Quinn

Publicly Accessible Penn Dissertations

Persistent pain is a common occurrence following whiplash injuries produced during motor vehicle crashes. The cervical facet joint and its capsule have often been identified as the source of chronic pain in patients with whiplash-associated disorders. However, for the majority of patients, no radiographic evidence of cervical spine injury is present. A capsular ligament stretch-based mechanism for initiating facet-mediated pain has been proposed based on human cadaveric studies of the facet joint kinematics during whiplash stimulations. However, without direct evidence of capsule damage during whiplash, the biomechanical and physiological mechanisms by which altered vertebral kinematics produce a facet capsule injury ...


Development As A New Paradigm For Improved Tendon Healing: A Characterization Of Neonatal Tendon Development, Healing During Development And An Investigation Into Differential Parameters During Accelerated Healing, Heather L. Ansorge Dec 2010

Development As A New Paradigm For Improved Tendon Healing: A Characterization Of Neonatal Tendon Development, Healing During Development And An Investigation Into Differential Parameters During Accelerated Healing, Heather L. Ansorge

Publicly Accessible Penn Dissertations

During neonatal development, tendons undergo a well orchestrated process whereby extensive structural and compositional changes occur in synchrony to produce a normal tissue. Conversely, during the repair response to injury, structural and compositional changes occur, but in this case, a mechanically inferior tendon is produced. An injured tendon that is mechanically inferior has compromised function and reruptures after treatment are commonly observed clinically. As a result, the process of development has been postulated as a potential paradigm through which improved adult tissue healing may occur. First, the role of CD44 in healing was examined through a patellar tendon injury in ...


Mammary Epithelial Metastatic Phenotype Forced Through The Extracellular Matrix, Olga Shebanova Dec 2010

Mammary Epithelial Metastatic Phenotype Forced Through The Extracellular Matrix, Olga Shebanova

Publicly Accessible Penn Dissertations

Biochemical and mechanical cues of the extracellular matrix have been shown to play important roles in cell-matrix and cell-cell interactions. We have experimentally tested the combined influence of these cues on cell motility, cell-cell interaction and assembly and traction force profile in an in vitro breast cancer model. The behavior of non-tumorigenic mammary epithelial cells was observed on surfaces with varying ligand concentration and polyacrylamide gel rigidity. Our data shows that cell velocity is biphasic in both matrix rigidity and adhesiveness, which are inversely related. Traction force microscopy revealed that maximum migration velocity is reached at intermediate force of single ...


Dendritic Cell Migration And Traction Force Generation In Engineered Microenvironments, Brendon Guenther Ricart Dec 2010

Dendritic Cell Migration And Traction Force Generation In Engineered Microenvironments, Brendon Guenther Ricart

Publicly Accessible Penn Dissertations

Dendritic cells (DCs) are potent initiators of the adaptive immune response. Their trafficking from sites of inflammation to lymphoid tissue is essential to their function. Exactly how dendritic cells integrate multiple chemotactic cues to organize an accurate migratory path is not fully understood. We first characterize DC random motility (chemokinesis) on extracellular matrix proteins in the presence of chemokines. Then, using a microfluidic device, we present both single and competing chemokine gradients to murine bone-marrow derived DCs in a controlled, time-invariant microenvironment. We show that in counter gradients, CCL19 is 10 to 100 fold more potent than other chemokines CCL21 ...


Microbiorobots For Single Cell Manipulation, Mahmut Selman Sakar Nov 2010

Microbiorobots For Single Cell Manipulation, Mahmut Selman Sakar

Publicly Accessible Penn Dissertations

One of the great challenges in nano and micro scale science and engineering is the independent manipulation of biological cells and small man-made objects with active sensing. For such biomedical applications as single cell manipulation, telemetry, and localized targeted delivery of chemicals, it is important to fabricate microstructures that can be powered and controlled without a tether in fluidic environments. These microstructures can be used to develop microrobots that have the potential to make existing therapeutic and diagnostic procedures less invasive.

Actuation can be realized using various different organic and inorganic methods. Previous studies explored different forms of actuation and ...


Prediction Of Proapoptotic Anticancer Therapeutic Response Based On Visualization Of Death Ligand-Receptor Interaction And Specific Marker Of Cellular Proliferation, Lanlan Zhou Aug 2010

Prediction Of Proapoptotic Anticancer Therapeutic Response Based On Visualization Of Death Ligand-Receptor Interaction And Specific Marker Of Cellular Proliferation, Lanlan Zhou

Publicly Accessible Penn Dissertations

Emerging targeted therapeutics hold great promise for the treatment of human cancer. However there are still challenges for selecting patients that most likely will benefit from targeted drugs. One of the major limitations of classical imaging methods is the significant delay to provide quantifiable and objective evidence of response to cancer therapy. Molecular imaging may be useful in targeted drug development by assessing the target expression and drug-target interaction, and predicting therapeutic response in both preclinical and clinical settings. The apoptosis pathway triggered by the Tumor Necrosis Factor (TNF)-Related Apoptosis-Inducing Ligand (TRAIL) receptors is a potential target for therapeutic ...


Enhancing Mesenchymal Stem Cell Chondrogenesis For Cartilage Tissue Engineering, Alice H. Huang Aug 2010

Enhancing Mesenchymal Stem Cell Chondrogenesis For Cartilage Tissue Engineering, Alice H. Huang

Publicly Accessible Penn Dissertations

Articular cartilage provides a bearing surface for transmitting forces across joints. The poor ability of cartilage to self-repair has motivated efforts to engineer replacement tissues, and mesenchymal stem cells (MSCs), which can undergo chondrogenesis, have emerged as a promising cell source. To date however, the functional properties of MSC-based constructs remain lower than those of the native tissue and of chondrocyte-based constructs cultured identically. Therefore, the overall objective of this thesis is to better understand the transcriptional and functional limitations underlying chondrogenic differentiation and enhance MSC chondrogenesis.

Toward this end, established tissue engineering strategies from the chondrocyte literature were applied ...


The Role Of Altered Loading In Pathological Changes In The Long Head Of The Biceps Tendon Following Rotator Cuff Tears In A Rat Model, Cathryn D. Peltz Aug 2010

The Role Of Altered Loading In Pathological Changes In The Long Head Of The Biceps Tendon Following Rotator Cuff Tears In A Rat Model, Cathryn D. Peltz

Publicly Accessible Penn Dissertations

Damage to the biceps tendon is common clinically and is frequently seen in the presence of rotator cuff tears. However, there is some debate over the role of the biceps tendon at the shoulder following a rotator cuff tear with some believing the biceps to play a significant role as a humeral head depressor while others believe the biceps plays no role at all. Therefore, controversy exists regarding its optimal treatment, with physicians relying mostly on anecdotal experience. Unfortunately, most clinical studies are not able to address the underlying causes in a controlled manner and cadaveric studies cannot monitor the ...


Combinatorial Polymer Synthesis And High-Throughput Screening Technology To Identify Optimal Approaches For Mineralized Tissue Engineering, Darren M. Brey Aug 2010

Combinatorial Polymer Synthesis And High-Throughput Screening Technology To Identify Optimal Approaches For Mineralized Tissue Engineering, Darren M. Brey

Publicly Accessible Penn Dissertations

The general tissue engineering approach is to combine cells, scaffolding, and signaling molecules in a manner that treats damaged or diseased tissues. Progress in the field has been made incrementally since changes to these inputs require meticulous iterative steps. However, recent advances in combinatorial chemistry and high through-put screening (HTS) technology have made available a wider range of potential scaffold materials with diverse properties, as well as new methods to accelerate the process of testing soluble factors or combinations of factors.

To this end, a library of poly(β-amino ester)s has been developed as the first combinatorial library of ...


Quantitative Molecular Mri Of Intervertebral Disc Degeneration, Chenyang Wang Aug 2010

Quantitative Molecular Mri Of Intervertebral Disc Degeneration, Chenyang Wang

Publicly Accessible Penn Dissertations

Degeneration of the intervertebral disc (IVD) is the most common cause of back-related disability among North American adults. Low-back-pain and associated disability costs the United States more than 100 billion dollars annually in health care expenditures and reduced productivity. The mechanism of IVD degeneration, especially its biomolecular aspect, is poorly understood in an in vivo setting. Thus there is increasingly a need for the non-invasive diagnosis and quantification of IVD degeneration. MRI is a non-invasive imaging modality capable of producing contrast sensitive to biomolecules. Therefore, the primary objective of this dissertation research project is to develop MRI techniques capable of ...


Characterizing Connectivity In Brain Networks Using Magnetic Resonance Imaging, Jeffrey T. Duda Aug 2010

Characterizing Connectivity In Brain Networks Using Magnetic Resonance Imaging, Jeffrey T. Duda

Publicly Accessible Penn Dissertations

The human brain is a system of neurons that provides great computational capacity via a complicated communication network. A complete description of this network, also known as the human connectome, is an active topic of research as it would provide invaluable knowledge to cognitive neuroscience. Historically, the study of brain connectivity has been marked by a dichotomy between structure and function. Recent advances in magnetic resonance imaging (MRI) provide a potential framework for bridging this divide and allowing for a more complete window onto brain connectivity. Here, a framework for examining structural connectivity and functional connectivity in a network is ...


Meniscus Tissue Engineering With Nanofibrous Scaffolds, Brendon M. Baker Aug 2010

Meniscus Tissue Engineering With Nanofibrous Scaffolds, Brendon M. Baker

Publicly Accessible Penn Dissertations

The fibrocartilaginous menisci dwell between the articular surfaces of the knee and play a central role in joint function. Damage through trauma or degenerative changes is a common orthopaedic injury, disrupts the meniscus mechanical function, and leads to the precocious development of osteoarthritis. The current standard of treatment is removal of the damaged tissue, a procedure that does not re-establish normal knee mechanics. Given the frequency of meniscal injury, a restorative repair strategy remains a major unmet need in orthopaedic medicine. With the goal of replacing damaged meniscus with engineered biologic tissue that can restore meniscal function, this work focuses ...


Integrating Theoretical And Experimental Methods For Multi-Scale Tissue Engineering Of The Annulus Fibrosus Of The Intervertebral Disc, Nandan L. Nerurkar Aug 2010

Integrating Theoretical And Experimental Methods For Multi-Scale Tissue Engineering Of The Annulus Fibrosus Of The Intervertebral Disc, Nandan L. Nerurkar

Publicly Accessible Penn Dissertations

There is a critical need for tissue engineered replacements for diseased and degenerated intervertebral discs in order to assuage low back pain while restoring function to the spine. Despite progress by many research groups, it remains a challenge to engineer a replacement tissue that can withstand the complex, demanding loading environment of the spine. Due to the hierarchical organization of the intervertebral disc, successful recapitulation of its functional behavior requires replication of anatomic form and physiologic function over a wide range of length scales. In this work, the technology of electrospinning has been employed for tissue engineering of the annulus ...


Acellular And Radically Polymerized Biodegradable Materials To Control Tissue Interactions After Myocardial Infarction, Jamie L. Ifkovits Aug 2010

Acellular And Radically Polymerized Biodegradable Materials To Control Tissue Interactions After Myocardial Infarction, Jamie L. Ifkovits

Publicly Accessible Penn Dissertations

Left ventricular remodeling following myocardial infarction (MI) often induces congestive heart failure, which has limited treatment options. Advances in tissue engineering and polymer chemistry, however have allowed for the development of alternative treatment strategies. Specifically, the formation of biodegradable polymers into complex scaffolds or injectable materials is being evaluated. The underlying hypothesis for this work is that fibrous scaffolds with controlled properties and structures, as well as injectable hydrogels with tunable properties, can be optimized to attenuate this post-MI remodeling response. To this end, four specific aims have been developed to test this global hypothesis.

First, a collection of biodegradable ...


Polymersomes: Multi-Functional Tools For In Vivo Cancer Theranostic Applications, Dalia H. Levine May 2010

Polymersomes: Multi-Functional Tools For In Vivo Cancer Theranostic Applications, Dalia H. Levine

Publicly Accessible Penn Dissertations

ABSTRACT

POLYMERSOMES: MULTI-FUNCTIONAL TOOLS FOR IN VIVO CANCER THERANOSTIC APPLICATIONS

Dalia Hope Levine

Dr. Daniel A. Hammer

Nanoparticles are currently being developed as delivery vehicles for therapeutic and contrast imaging agents. Polymersomes (mesoscopic polymer vesicles) possess a number of attractive biomaterial properties, including greater biocompatibility, prolonged circulation times, and increased mechanical stability, that make them ideal for these applications. The polymersome architecture, with its large hydrophilic reservoir and thick hydrophobic lamellar membrane, provides significant storage capacity for water soluble and insoluble substances.

The primary thesis aims are to develop multi-functional polymersomes for combination therapeutic applications, as well as simultaneous therapeutic ...