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Full-Text Articles in Biomedical Engineering and Bioengineering
The Co-Delivery Of Syngeneic Adipose-Derived Stromal Cells And Macrophages On Decellularized Adipose Tissue Bioscaffolds For In Vivo Soft Tissue Regeneration, Hisham A. Kamoun
The Co-Delivery Of Syngeneic Adipose-Derived Stromal Cells And Macrophages On Decellularized Adipose Tissue Bioscaffolds For In Vivo Soft Tissue Regeneration, Hisham A. Kamoun
Electronic Thesis and Dissertation Repository
Decellularized adipose tissue (DAT) bioscaffolds are a promising platform for the delivery of pro-regenerative cell populations with the goal of promoting adipose tissue regeneration. The current study investigated the effects of seeding DAT bioscaffolds with syngeneic bone marrow-derived macrophages and/or adipose-derived stromal cells (ASCs) on in vivo soft tissue regeneration. Methods were established to derive the macrophages from MacGreen mice, which were dynamically seeded onto the DAT scaffolds alone or in combination with ASCs. Seeded and unseeded scaffolds were implanted subcutaneously into C57Bl/6 mice. At 2 and 4 weeks, cell infiltration, angiogenesis, and adipogenesis were analyzed through histology and immunohistochemistry. …
Syngenic Adipose-Derived Stem/Stromal Cells Delivered In Decellularized Adipose Tissue Scaffolds Enhance In Vivo Tissue Regeneration Through Host Cell Recruitment, Kevin P. Robb
Electronic Thesis and Dissertation Repository
Decellularized adipose tissue (DAT) represents a promising adipogenic bioscaffold for applications in soft tissue augmentation or reconstruction. With the goal of investigating the role of syngeneic donor adipose-derived stem/stromal cells (ASCs) and host myeloid cells during in vivo adipose tissue regeneration, transgenic reporter mouse strains were used to track these cell populations within ASC-seeded and unseeded DAT scaffolds. Donor ASCs were obtained from dsRed transgenic mice. These cells were shown to express characteristic cell surface markers, and multilineage differentiation capacity was confirmed. To facilitate cell tracking, DAT scaffolds were subcutaneously implanted into MacGreen mice in which myeloid cells express enhanced …
Adipose-Derived Stem Cell Differentiation In Cell Aggregates Supplemented With Micronized, Decellularized Extracellular Matrix, Danielle Heinbuch
Adipose-Derived Stem Cell Differentiation In Cell Aggregates Supplemented With Micronized, Decellularized Extracellular Matrix, Danielle Heinbuch
Electronic Thesis and Dissertation Repository
In the human body there are tissues with limited regenerative potential, raising the need for cell-based regenerative therapies. The main objective of this thesis was to examine how tissue-specific extracellular matrix (ECM) affects the differentiation of human adipose-derived stem/stromal cells (ASCs). The effects of incorporating milled, decellularized adipose (DAT) or cartilage (DCT) ECM particles on ASC lineage-specific differentiation in 3-D cell aggregate cultures were explored. The results demonstrated that the addition of ECM improved differentiation for both adipogenesis and chondrogenesis. Analysis of adipogenic gene and protein expression indicated enhanced differentiation in the DAT+ASC aggregates relative to the DCT+ASC and ASC-alone …