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Biomedical Engineering and Bioengineering Commons

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Theses/Dissertations

Life Sciences

2003

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Full-Text Articles in Biomedical Engineering and Bioengineering

Cloning, Expression, And Characterization Of Gfp:Xkcm1 Deletion Constructs, Patrick Thorsen Apr 2003

Cloning, Expression, And Characterization Of Gfp:Xkcm1 Deletion Constructs, Patrick Thorsen

Life and Environmental Sciences Undergraduate Theses

Kinesins are a type of motor protein that use the energy of ATP hydrolysis to move along microtubules. Kinesin Catastrophe Modulator One (XKCM1) of Xenopus laevis was recently identified as a kinesin that is essential for the establishment and maintenance of mitotic spindles by affecting the microtubule dynamics in Xenopus egg extracts. In the present research, three different deletion constructs of XKCM1 fused with Green Flourescent Protein (GFP) were cloned, expressed, and characterized in order to determine which part of the XKCM1 structure was necessary for proper destabilization of microtubules. These deletion constructs have previously been studied z'n vivo ...