Open Access. Powered by Scholars. Published by Universities.®

Biomedical Engineering and Bioengineering Commons

Open Access. Powered by Scholars. Published by Universities.®

Theses/Dissertations

Life Sciences

Retrospective Theses and Dissertations

Mechanical engineering

Articles 1 - 1 of 1

Full-Text Articles in Biomedical Engineering and Bioengineering

Force Interaction Characterization Between Thrombin And Dna Aptamers, Janice Dionne Marquardt Jan 2008

Force Interaction Characterization Between Thrombin And Dna Aptamers, Janice Dionne Marquardt

Retrospective Theses and Dissertations

The first purpose of this study is to examine using AFM height measurements to detect changes in nucleic acid and protein interactions. This study used a thiolated adenine oligonucleotide (Poly A) and thymine DNA strand (Poly T) or 2 kDa poly(ethylene) glycol (PEG) as control, and the thrombin aptamer and thrombin for detection pair. The poly A and aptamer were printed using microcontact printing, then exposed to complementary species. There was a height change after binding to the target species, so it is feasible for height data to detect binding. The second purpose of this study is to use ...