Biomedical Engineering and Bioengineering Commons^™

1-Nitronaphthalene Causes Ultrastructural Changes Primarily In Nonciliated Bronchiolar Epithelium Followed By Ciliated Epithelium In The Rat, John-Michael Sauer

Masters Theses

This study describes morphological changes in the lung at several time intervals following a single injection of 1-Nitronaphthalene (100 mg/kg intraperitoneal) in male Sprague-Dawley rats using transmission and scanning electron microscopy. Dilation of the smooth endoplasmic reticulum and mitochondrial distension in the nonciliated bronchiolar (Clara) cells was apparent as early as 1 hour after injection, while adjacent ciliated cell showed minimal changes. At 6 hours, when respiratory distress was apparent in the animals, Clara and ciliated cells exhibited chromatin clumping, loss of mitochondrial cristae and cytoplasmic vacuolization. Also at 6 hours, the interstitial area around the terminal bronchioles was infiltrated …