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Biomedical Engineering and Bioengineering Commons

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Theses/Dissertations

Life Sciences

University of Colorado, Boulder

Cell

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Full-Text Articles in Biomedical Engineering and Bioengineering

In Situ Designer Lipid Production: Integration Of Novel Characteristics And Behaviors Into Synthetic Cell Membranes, Danielle S. Konetski Jan 2018

In Situ Designer Lipid Production: Integration Of Novel Characteristics And Behaviors Into Synthetic Cell Membranes, Danielle S. Konetski

Chemical & Biological Engineering Graduate Theses & Dissertations

This thesis investigated the coupling of lysolipids and functionalized tails for in situ formation of synthetic liposomes designed to enable specific characteristics or behaviors in applications ranging from drug delivery to the advancement of artificial cell development. Copper-catalyzed Azide-Alkyne Cycloaddition (CuAAC) mediated lipid coupling was improved via incorporation of a photoinitiation system. Here, photo-CuAAC enabled spatiotemporal control over liposome assembly, an over 400-fold increase in formation density, and control over the maximal cross-sectional area of the liposomes formed.

Thiol-Michael mediated lipid coupling was enabled using thiol-functionalized lysolipids and acrylate tails where phospholipids were produced over 48 hours with approximate 90 ...