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Biomedical Engineering and Bioengineering Commons

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Theses/Dissertations

Life Sciences

University of Colorado, Boulder

Biofuels

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Full-Text Articles in Biomedical Engineering and Bioengineering

Engineering Bacterial Gene Expression: Applications Towards Biofuels And Antibiotic Resistance, Peter Britton Otoupal Jan 2018

Engineering Bacterial Gene Expression: Applications Towards Biofuels And Antibiotic Resistance, Peter Britton Otoupal

Chemical & Biological Engineering Graduate Theses & Dissertations

Nature is rarely static. Organisms live in diverse and stressful environments that necessitate rapid response strategies for survival. Microorganisms have responded to this by evolving bet-hedging, wherein they exhibit constitutively heterogeneous gene expression to maximize fitness across numerous background. The goal of this thesis is to “hijack” this phenomenon using novel gene expression engineering techniques to alter how bacteria respond to their environments, in order to address pressing societal concerns.

This begins with a systematic exploration of how bacterial gene expression naturally responds to antibiotics and biofuels. This reveals promising gene candidates for targeted manipulation, for which a library of ...