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Theses/Dissertations

Life Sciences

University of Colorado, Boulder

Bacteriophage

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Engineering Of Filamentous Bacteriophage For Protein Sensing, Michael David Brasino Jan 2016

Engineering Of Filamentous Bacteriophage For Protein Sensing, Michael David Brasino

Materials Science & Engineering Graduate Theses & Dissertations

Methods of high throughput, sensitive and cost effective quantification of proteins enables personalized medicine by allowing healthcare professionals to better monitor patient condition and response to treatment. My doctoral research has attempted to advance these methods through the use of filamentous bacteriophage (phage). These bacterial viruses are particularly amenable to both genetic and chemical engineering and can be produced efficiently in large amounts. Here, I discuss several strategies for modifying phage for use in protein sensing assays. These include the expression of bio-orthogonal conjugation handles on the phage coat, the incorporation of specific recognition sequences within the phage genome, and ...