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Biomedical Engineering and Bioengineering Commons

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Theses/Dissertations

Life Sciences

University of Colorado, Boulder

Affinity Selection

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Full-Text Articles in Biomedical Engineering and Bioengineering

A Novel Selection Technology For The Discovery Of High-Affinity Human Proteins, David Busha Jan 2014

A Novel Selection Technology For The Discovery Of High-Affinity Human Proteins, David Busha

Molecular, Cellular, and Developmental Biology Graduate Theses & Dissertations

Proteins that bind with high-affinity to cellular targets can be useful therapeutic treatments. High-throughput affinity screening of large protein libraries is often more successful at discovering novel high-affinity proteins than rational-design approaches. Display techniques such and phage and yeast display are commonly used in this screening process. However, bacteria and yeast cells can misfold or otherwise inappropriately express mammalian and human proteins due to differences in codon usage, protein folding machinery and post-translational modifications. Therefore, a display system that is entirely based in human cells could aid in the discovery of novel, high-affinity proteins. No such system has been described ...