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Biomedical Engineering and Bioengineering Commons

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Full-Text Articles in Biomedical Engineering and Bioengineering

The Effects Of Weak Static And Pulsed Magnetic Fields On Fibroblast And Fibrosarcoma Cells Under A Controlled Magnetic Environment, Julian Brandon Cyrus Apr 2015

The Effects Of Weak Static And Pulsed Magnetic Fields On Fibroblast And Fibrosarcoma Cells Under A Controlled Magnetic Environment, Julian Brandon Cyrus

Electrical, Computer & Energy Engineering Graduate Theses & Dissertations

This thesis provides evidence supporting the claim that weak static and pulsed magnetic fields have measureable effects upon fibrosarcoma cells. Fibroblast and fibrosarcoma cell lines were exposed to 0, 100, and 200μT fields, with a static 45μT field as the control. Each exposure intensity, other than 0μT, was conducted at a 20 sec pulse repetition rate, as well as the normal static conditions. The cells were tested for variations in oxidative stress, membrane potential, and cell viability. The fibrosarcoma cells were also tested for cell count. The trials were conducted using an electromagnetic system that provided a controlled magnetic environment ...


Optimization And Control Of An Active Helmet Design For Head Injury Prevention, Chadi S. Abdel-Samad Apr 2015

Optimization And Control Of An Active Helmet Design For Head Injury Prevention, Chadi S. Abdel-Samad

Electrical, Computer & Energy Engineering Graduate Theses & Dissertations

Thousands of mild traumatic brain injuries (mBTIs) occur in US football each year. In some instances, injuries are quite serious and life-threatening. New NFL rules being enforced by the league are an attempt to address this problem. Of interest is whether an active helmet design, employing feedback, can also reduce the incidence of mBTIs in football. Medical evidence and research suggests that peak center of gravity (CG) head acceleration is well correlated with the onset and severity of mBTI. In this dissertation, an active helmet design is introduced, dynamically modeled, and explored from the perspective of using control to reduce ...


Improved Methods For Understanding Sparse, Multi-Dimensional, High Throughput Sequencing Data, Sophie J. Weiss Jan 2015

Improved Methods For Understanding Sparse, Multi-Dimensional, High Throughput Sequencing Data, Sophie J. Weiss

Chemical & Biological Engineering Graduate Theses & Dissertations

Tremendous advances in genetic and sequencing technology are enabling unprecedented insight into human disease, forensics, and cellular mechanisms, to name a few. Conclusions drawn from these studies are strongly influenced by the interpretation of their associated massive data sets. The goal of this thesis is to understand, develop, and apply algorithms to help overcome common ecological and biological sequencing study challenges: contamination, differences in sampling efforts, a very large amount of zeroes, and compositionality.

We use simulations and experimental data to understand how different matrix normalization strategies mitigate the effects of the aforementioned challenges on downstream analyses, particularly principal coordinate ...


The Role Of Matrix Properties In Directing Valvular Interstitial Cell Phenotype, Kelly Marie Pollock Mabry Jan 2015

The Role Of Matrix Properties In Directing Valvular Interstitial Cell Phenotype, Kelly Marie Pollock Mabry

Chemical & Biological Engineering Graduate Theses & Dissertations

This thesis presents the development of hydrogel platforms to study the fibroblast-to-myofibroblast transition in valvular interstitial cells (VICs). These systems were used to characterize the effects of extracellular matrix cues on VICs, as well as the synergies between mechanical and biochemical signals. First, the impact of culture platform on VIC phenotype was assessed by culturing VICs in peptide-functionalized poly(ethylene glycol) hydrogels (2D and 3D) and comparing them to those cultured on tissue culture polystyrene (TCPS). Expression of the myofibroblast marker α-smooth muscle actin (αSMA), as well as by a global analysis of the transcriptional profiles1 demonstrated that TCPS ...