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Full-Text Articles in Biomedical Engineering and Bioengineering
Improved Methods For Understanding Sparse, Multi-Dimensional, High Throughput Sequencing Data, Sophie J. Weiss
Chemical & Biological Engineering Graduate Theses & Dissertations
Tremendous advances in genetic and sequencing technology are enabling unprecedented insight into human disease, forensics, and cellular mechanisms, to name a few. Conclusions drawn from these studies are strongly influenced by the interpretation of their associated massive data sets. The goal of this thesis is to understand, develop, and apply algorithms to help overcome common ecological and biological sequencing study challenges: contamination, differences in sampling efforts, a very large amount of zeroes, and compositionality.
We use simulations and experimental data to understand how different matrix normalization strategies mitigate the effects of the aforementioned challenges on downstream analyses, particularly principal coordinate ...