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Full-Text Articles in Biomedical Engineering and Bioengineering

Impact Of Collateral Enlargement On Smooth Muscle Phenotype, Alexander Jerome Bynum Dec 2011

Impact Of Collateral Enlargement On Smooth Muscle Phenotype, Alexander Jerome Bynum

Master's Theses and Project Reports

Peripheral Artery Disease is a very serious disease characterized by an arterial occlusion due to atherosclerotic plaques. In response to an arterial occlusion, arteriogenesis occurs, causing smooth muscle cells to transition from a contractile to synthetic state. Also following an arterial occlusion, functional impairment was seen in the collateral circuit. An immunofluorescence protocol was developed in order to assess the impact of collateral enlargement (arteriogenesis) on smooth muscle phenotype at various time points. Smooth muscle α-actin was used to mark all smooth muscle cells, Ki-67 was used to label proliferating smooth muscle cells, and a fluorescent nuclear stain was used ...


Impaired Resistance Artery Reactivity Following Arteriogenesis, Michael (Mike) Machado Sep 2011

Impaired Resistance Artery Reactivity Following Arteriogenesis, Michael (Mike) Machado

Biomedical Engineering

No abstract provided.


Determining A Method For Rendering Low Cost Cdse(Zns) Core(Shell) Quantum Dots Aqueous Soluble Via Amphiphilic Polymer Wrapping, Patrick Mcbride Jun 2011

Determining A Method For Rendering Low Cost Cdse(Zns) Core(Shell) Quantum Dots Aqueous Soluble Via Amphiphilic Polymer Wrapping, Patrick Mcbride

Materials Engineering

Herein is described the procedure of two amphiphilic polymer wrapping techniques that may be employed for obtaining aqueous soluble quantum dots (QDs) for use in biological fluorescent imaging applications. The advent of QDs has led to new nanoscale fluorescent materials that exhibit unparalleled quantum yields (QYs), high resistance to photobleaching, tunable emissions, and
absorption over a large optical range. However, the QD synthesis employed here at Cal Poly to obtain bright, photostable CdSe(ZnS) core(shell) QDs involves the use of organic solvents and surfactants, leading to hydrophobic QDs. Since all of biology relies on aqueous solubility, this hydrophobicity creates ...


Ischemia Impairs Vasodilation In Skeletal Muscle Resistance Artery, Kyle Remington Struthers Jun 2011

Ischemia Impairs Vasodilation In Skeletal Muscle Resistance Artery, Kyle Remington Struthers

Master's Theses and Project Reports

Functional vasodilation in arterioles is impaired with chronic ischemia. We sought to examine the impact of chronic ischemia and age on skeletal muscle resistance artery function. To examine the impact of chronic ischemia, the femoral artery was resected from young (2-3mo) and adult (6-7mo) mice and the profunda femoris artery diameter was measured at rest and following gracilis muscle contraction 14 days later using intravital microscopy. Functional vasodilation was significantly impaired in ischemic mice (14.4±4.6% vs. 137.8±14.3%, p<0.0001 n=8) and non-ischemic adult mice (103.0±9.4% vs. 137.8±14.3%, p=0.05 n=10). In order to analyze the cellular mechanisms of the impairment, a protocol was developed to apply pharmacological agents to the experimental preparation while maintaining tissue homeostasis. Endothelial and smooth muscle dependent vasodilation were impaired with ischemia, 39.6 ± 13.6% vs. 80.5 ± 11.4% and 43.0 ± 11.7% vs. 85.1 ± 10.5%, respectively. From this data, it can be supported that smooth muscle dysfunction is the reason for the observed impairment in arterial vasodilation.


Studying Milk Coagulation Kinetics With Laser Scanning Confocal Microscopy, Image Processing, And Computational Modeling, Richard Joseph Hennessy Jun 2011

Studying Milk Coagulation Kinetics With Laser Scanning Confocal Microscopy, Image Processing, And Computational Modeling, Richard Joseph Hennessy

Master's Theses and Project Reports

The kinetics of milk coagulation are complex and still not well understood. A deeper understanding of coagulation and the impact of the relevant factors would aid in both cheese manufacturing and also in determining the nutritional benefits of dairy products. A method using confocal microscopy was developed to follow the movement of milk fat globules and the formation of a milk protein network during the enzyme-induced coagulation of milk. Image processing methods were then used to quantify the rate of coagulation. It was found that the texture of the protein network is an indicator of the current status of the ...


A Novel Exercise Device For Users In Wheelchairs: A Study Of Abdominal Muscle Activation, Rebecca Jo Campbell Jun 2011

A Novel Exercise Device For Users In Wheelchairs: A Study Of Abdominal Muscle Activation, Rebecca Jo Campbell

Master's Theses and Project Reports

This study evaluates the use of a wheelchair balance board. The balance board was created as a sensory stimulation tool for users with various disabilities. It was originally designed to create vestibular stimulation for the person after they were loaded on. This study was used as a way to test if the balance board could be used for other things such as physical therapy and exercise. Ten able-bodied individuals were used to show the functionality of the device. They were asked to perform six different abdominal exercises while the muscle activity of their rectus abdominis and external obliques was measured ...