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Full-Text Articles in Biomedical Engineering and Bioengineering

Characterization Of Oxone Mediated Tempo-Oxidized Nano Cellulose Mixed-Matrix Membranes During Ultrafiltration And Hemodialysis, Kristyn Robling May 2020

Characterization Of Oxone Mediated Tempo-Oxidized Nano Cellulose Mixed-Matrix Membranes During Ultrafiltration And Hemodialysis, Kristyn Robling

Biomedical Engineering Undergraduate Honors Theses

The ninth leading cause of death in the United States is kidney disease, and hemodialysis is the process most commonly prescribed for treatment. It utilizes a selectively permeable membrane filter to remove toxins such as urea from the blood and retain necessary protein levels. However, traditional filters, such as cellulose triacetate, used during dialysis can be inefficient in terms of separation performance and reduction of fouling. Recent exploration of nanoparticles has resulted in the creation of Oxone Mediated TEMPO-Oxidized Nano Cellulose which has properties that are believed to increase hydrophilicity, increase tensile capacity, decrease membrane resistance and lower fouling, making ...


Investigation Of The Electrode Polarization Effect For Biosensor Applications, Anil Koklu Aug 2019

Investigation Of The Electrode Polarization Effect For Biosensor Applications, Anil Koklu

Mechanical Engineering Research Theses and Dissertations

My research focuses on electrokinetic transport. Particularly, in this dissertation, we focus on fabrication and testing of micro electrodes with nanostructured surfaces to minimize the electrode polarization (EP) effects for biosensor applications. In the first study, electrochemical deposition of gold nanoparticles on to planar gold electrodes was used to generate rough surfaces. Dendritic nanostructures that reduced EP up to two orders of magnitude was obtained by optimizing the deposition conditions. These structures also enhanced dielectrophoresis (DEP) response of our bio-chips, making them usable in physiological buffers. In further studies we discovered a universal scaling of EP in the frequency domain ...


Bioengineering Extracellular Matrix Scaffolds For Volumetric Muscle Loss, Kevin Roberts Aug 2019

Bioengineering Extracellular Matrix Scaffolds For Volumetric Muscle Loss, Kevin Roberts

Theses and Dissertations

Volumetric muscle loss overwhelms skeletal muscle’s ordinarily capable regenerative machinery, resulting in fibrosis and severe functional deficits which have defied clinical repair strategies. My work spans the design and preclinical evaluation of implants intended to drive the cell community of injured muscle toward a regenerative state, as well as the development of an understanding of the molecular responses of this cell community to biomaterial interventions. I demonstrate a new class of biomaterial by leveraging the productive capacity of sacrificial hollow fiber membrane cell culture; I show specifically that unique threads of whole extracellular matrix can be isolated by solvent ...


Fabrication Of Enhanced Carbon Based Biocompatible And Biodegradable Microelectronic Materials Derived From Lignocellulosic Biomass, Harrison Thomas Hawkins May 2019

Fabrication Of Enhanced Carbon Based Biocompatible And Biodegradable Microelectronic Materials Derived From Lignocellulosic Biomass, Harrison Thomas Hawkins

Theses and Dissertations

The development of materials capable of harmlessly being broken down and removed from the body is a crucial step towards the development of short-term application electronic biomedical implants. Once developed, these implants, known as bioresorbable electronics, will open a wide array of temporary applications in the field of biomedical implantable devices. Necessary to the operation of bioresorbable electronics within the body is a power source that is similarly biocompatible and biodegradable. To this end, enhanced carbon-based materials and a bio-ionic liquid were developed for the fabrication of a preliminary implantable and bioresorbable battery and tested for functional properties. Electrodes were ...


Diagnostics And Selective Nano-Therapeutics To Combat Multidrug-Resistant Bacteria, Partha Pratim Chowdhury Jan 2019

Diagnostics And Selective Nano-Therapeutics To Combat Multidrug-Resistant Bacteria, Partha Pratim Chowdhury

Chemical & Biological Engineering Graduate Theses & Dissertations

Due to extensive use of antibiotics various strains multidrug resistant bacteria are emerging rapidly. In USA alone according to CDC report 2013 more than 2 million is affected by these strains of multidrug resistant bacteria causing several deaths. Hence an effective way of diagnostics and therapeutics need to be designed which can successfully combat the menace of

multidrug drug resistant bacteria.

In the present thesis, we discuss about novel strategies for both diagnostics and therapeutics for multidrug resistant bacteria. In the 1st chapter we discuss a novel DNA sequencing technique utilizing purely optical spectroscopy techniques like surface enhanced Raman and ...


Fabrication And Characterization Of Nanofiber Nylon-6-Mwcnt As An Electrochemical Sensor For Sodium Ions Concentration Detection In Sweat, Kelsey Mills Jan 2019

Fabrication And Characterization Of Nanofiber Nylon-6-Mwcnt As An Electrochemical Sensor For Sodium Ions Concentration Detection In Sweat, Kelsey Mills

Williams Honors College, Honors Research Projects

Fabrication and characterization nylon-6-MWCNT nanofiber as an electrochemical sensor to detect sodium ion concentrations specifically in sweat. Using contact angle to determine surface morphology and chronoamperometry testing to identify ideal sensor conditions, tests optimized parameters like weight percent of nylon or other polymers, carbon nanotube (CNT) isomer, and solution concentration to determine reproducibility of functional sensors. Utilizing the electric qualities of carbon nanotubes partnered with the sodium ion selectivity of calixarene treatment and polymers unique properties like flexibility and scalability create open an arena for optimizing sodium ion sensors for further development for functional prototypes. Morphology tests showed that the ...


Effects Of Micro-Features On Cell Detachment From Poly(N-Isopropylacrylamide) Coated Polydimethylsiloxane Membranes, Luke Webel Jan 2019

Effects Of Micro-Features On Cell Detachment From Poly(N-Isopropylacrylamide) Coated Polydimethylsiloxane Membranes, Luke Webel

Williams Honors College, Honors Research Projects

The tested hypothesis was that features on polydimethylsiloxane (PDMS) surfaces coated with a poly(N-isopropylacrylamide)/aminopropyltriethoxysilane or pNIPAAM/APTES thin film would accelerate cell detachment than the film coated on a unfeatured surface. This project tested samples with features generated by molds, wrinkling, and sandpaper roughened substrates. Surface feature generation methods were limited to mechanical means, and characterized by microscopy and strain rates. 50/50 mixtures of 1.5 wt.% pNIPAAM/ APTES were used to coat thin films (30-40 nm) on PDMS membranes by spin-coating, and the coated membranes were thermally annealed to chemically graft pNIPAAm/APTES on the membrane ...


Towards The Rational Design And Application Of Polymers For Gene Therapy: Internalization And Intracellular Fate, Landon Alexander Mott Jan 2019

Towards The Rational Design And Application Of Polymers For Gene Therapy: Internalization And Intracellular Fate, Landon Alexander Mott

Theses and Dissertations--Chemical and Materials Engineering

Gene therapy is an approach for the treatment of acquired cancers, infectious disease, degenerative disease, and inherited genetic indications. Developments in the fields of immunotherapies and CRISPR/Cas9 genome editing are revitalizing the efforts to move gene therapy to the forefront of modern medicine. However, slow progress and poor clinical outcomes have plagued the field due to regulatory and safety concerns associated with the flagship delivery vector, the recombinant virus. Immunogenicity and poor transduction in certain cell types severely limits the utility of viruses as a delivery agent of nucleic acids. As a result, significant efforts are being made to ...


Nanoscale Stiffness Cues Influence Valvular Interstitial Cell Activation To Myofibroblasts, Michaela Wenning Jan 2019

Nanoscale Stiffness Cues Influence Valvular Interstitial Cell Activation To Myofibroblasts, Michaela Wenning

Undergraduate Honors Theses

Surgery is currently the primary treatment option for aortic valve stenosis (AVS) patients, many of whom are ineligible for surgery and are left untreated. AVS is progression is known to differ between males and females, and an understanding of sex-specific mechanisms of disease progression is imperative in developing accurate treatment options for men and women. The development of a nonsurgical therapy for AVS patients requires a deeper understanding of the molecular and cellular mechanisms of AVS progression. Currently, the role of calcium phosphate nanoparticles detected in the aortic valve during early stages of AVS in influencing disease progression and valvular ...


Modification Of Lipid Microenvironments On Solid Support Structures For Use In Transmembrane Protein Assays, William J. Houlihan Jan 2019

Modification Of Lipid Microenvironments On Solid Support Structures For Use In Transmembrane Protein Assays, William J. Houlihan

Dissertations and Theses

Gamma-Secretase (γ-secretase) is a transmembrane protease of increasing interest, which has been shown to have significant connections to both cancer and Alzheimer’s disease. γ-secretase cleaves both Notch-1, a transmembrane signaling protein, and Amyloid precursor protein (APP), a transmembrane protein whose cleavage may result in the formation of β-amyloid plaques in the brain. Notch-1 and APP are widely studied proteins that have substantial impacts on the development and proliferation of cancer and Alzheimer’s disease, respectively. Notch-1 partakes in the signaling of apoptosis in damaged and mutated cells, thus its cleavage by γ-secretase within the plasma membrane has ramifications on ...


Spatiotemporal Modeling And Model Restructuration Approaches In Studies Of Intracellular Signalling Pathways, Md Shahinuzzaman Jan 2019

Spatiotemporal Modeling And Model Restructuration Approaches In Studies Of Intracellular Signalling Pathways, Md Shahinuzzaman

Doctoral Dissertations

"The main focus of the research is to understand the complex phenomena of cell transduction pathways and cell biology in a single cell. Mathematical modeling and experimental evaluation are widely used approaches for this kind of research. Firstly, A multiscale framework for protein-protein interaction has been established using Brownian dynamics algorithm. Sit specific feature, steric collision, diffusion, co-localization and complex formation with time and space has been included in this spatial modeling framework. By implementation of the time adaptive feature in this framework, the computation time reduces in an order of magnitude compared with traditional modeling framework. This multiscale Brownian ...


Direct Quantification Of Deubiquitinating Enzyme Activity In Single Intact Cells, Nora Safabakhsh Aug 2018

Direct Quantification Of Deubiquitinating Enzyme Activity In Single Intact Cells, Nora Safabakhsh

LSU Doctoral Dissertations

Challenges in drug efficacy occur during the treatment of most types of cancer due to the heterogeneity of the tumor microenvironment. This has led to the development of personalized medicine. Due to the clinical success of the proteasome inhibitors Bortezomib and Carfilzomib in treatment of multiple myeloma, interest has shifted towards molecularly-targeted chemotherapeutics for ubiquitin-proteasome system (UPS). Deubiquitinating enzymes (DUBs) are an essential part of this pathway which have been found to promote Bortezomib resistance in multiple myeloma patients. Unfortunately, there is a lack of specific, high throughput biochemical assays to characterize DUB activity in patient samples before and after ...


Engineering Robust And Programmable Biological Systems, Tatenda Shopera Aug 2018

Engineering Robust And Programmable Biological Systems, Tatenda Shopera

Engineering and Applied Science Theses & Dissertations

The ability to engineer programmable biological systems using complex artificial gene networks has great potential to unlock important innovative solutions to many biotechnological challenges. While cells have been engineered to implement complex information processing algorithms and to produce food, materials, and pharmaceuticals, many innovative applications are yet to be realized due to our poor understanding of how robust, reliable, and predictable artificial gene circuits are built. In this work, we demonstrate that robust complex cellular behaviors (e.g., bistability and gene expression dynamics) can be achieved by engineering gene regulatory architecture and increasing the complexity of genetic networks. We further ...


Process Analytics From Passive Acoustic Emissions Monitoring During Fluidized Bed Pellet Coating In Pharmaceutical Manufacturing, Allan Carter Jun 2018

Process Analytics From Passive Acoustic Emissions Monitoring During Fluidized Bed Pellet Coating In Pharmaceutical Manufacturing, Allan Carter

Electronic Thesis and Dissertation Repository

Piezoelectric microphones were attached to a top spray fluidized bed to provide valuable process signatures. Relationships were developed between sound waves and conditions within the fluidized bed to relay critical quality and performance information. Deep learning analytics were used to extract valuable information from experimental data. Advancements in passive acoustic emissions monitoring will play a key role in optimizing pharmaceutical manufacturing pathways to ensure drug quality and performance.


Preparation Of Supramolecular Amphiphilic Cyclodextrin Bilayer Vesicles For Pharmaceutical Applications, Kate E. Frischkorn Jun 2018

Preparation Of Supramolecular Amphiphilic Cyclodextrin Bilayer Vesicles For Pharmaceutical Applications, Kate E. Frischkorn

Master's Theses and Project Reports

Recent pharmaceutical developments have investigated using supramolecular nanoparticles in order to increase the bioavailability and solubility of drugs delivered in various methods. Modification of the carbohydrate cyclodextrin increases the ability to encapsulate hydrophobic pharmaceutical molecules by forming a carrier with a hydrophobic core and hydrophilic exterior. Guest molecules are commonly added to these inclusion complexes in order to add stability and further increase targeting abilities of the carriers. One such guest molecule is adamantine combined with a poly(ethylene glycol) chain. Vesicles are formed by hydrating a thin film of amphiphilic cyclodextrin and guest molecules in buffer solution that mimics ...


Radical Social Ecology As Deep Pragmatism: A Call To The Abolition Of Systemic Dissonance And The Minimization Of Entropic Chaos, Arielle Brender May 2018

Radical Social Ecology As Deep Pragmatism: A Call To The Abolition Of Systemic Dissonance And The Minimization Of Entropic Chaos, Arielle Brender

Student Theses 2015-Present

This paper aims to shed light on the dissonance caused by the superimposition of Dominant Human Systems on Natural Systems. I highlight the synthetic nature of Dominant Human Systems as egoic and linguistic phenomenon manufactured by a mere portion of the human population, which renders them inherently oppressive unto peoples and landscapes whose wisdom were barred from the design process. In pursuing a radical pragmatic approach to mending the simultaneous oppression and destruction of the human being and the earth, I highlight the necessity of minimizing entropic chaos caused by excess energy expenditure, an essential feature of systems that aim ...


Antibacterial Properties Of A Cationic Imidazolium-Containing Poly(Ionic Liquid), Kaitlyn P. Brickey May 2018

Antibacterial Properties Of A Cationic Imidazolium-Containing Poly(Ionic Liquid), Kaitlyn P. Brickey

Biomedical Engineering Undergraduate Honors Theses

Poly(ionic liquids), or PILs, have been shown to have a number of biological applications as ligands, drug delivery vehicles, and nonviral DNA delivery mechanisms. Additionally, PILs have been proven to be efficient antibacterials. Imidazolium-containing PILs have promising results in antibacterial studies, but relating to their chain and charge density, only the effects of mono- & bis-imidazolium PILs have been sufficiently described in literature. The work detailed in this thesis aimed to further existing research on the effect of chain density by controlling the UV-initiator immobilization time. The effects of chain density were investigated by grafting poly(vinylimidazolium chloride) onto SiO ...


Covalently Crosslinked Organic/Inorganic Hybrid Biomaterials For Bone Tissue Engineering Applications, Dibakar Mondal Feb 2018

Covalently Crosslinked Organic/Inorganic Hybrid Biomaterials For Bone Tissue Engineering Applications, Dibakar Mondal

Electronic Thesis and Dissertation Repository

Scaffolds are key components for bone tissue engineering and regeneration. They guide new bone formation by mimicking bone extracellular matrix for cell recruitment and proliferation. Ideally, scaffolds for bone tissue engineering need to be osteoconductive, osteoinductive, porous, degradable and mechanically competent. As a single material can not provide all these requirements, composites of several biomaterials are viable solutions to combine various properties. However, conventional composites fail to fulfil these requirements due to their distinct phases at the microscopic level. Organic/inorganic (O/I) class II hybrid biomaterials, where the organic and inorganic phases are chemically crosslinked on a molecular scale ...


Thermal Fusion For Sutureless Closure: Devices, Composition, Methods, Eric Anthony Kramer Jan 2018

Thermal Fusion For Sutureless Closure: Devices, Composition, Methods, Eric Anthony Kramer

Mechanical Engineering Graduate Theses & Dissertations

As minimally invasive surgical techniques progress, the demand for reliable ligation is pronounced. The surgical advantages of energy-based vessel sealing exceed those of traditional, compression-based ligatures in procedures sensitive to duration, foreign bodies, and recovery time alike. While the use of energy-based devices to seal or transect vasculature and connective tissue bundles is widespread, the breadth of heating strategies and energy dosimetry used between devices underscores an uncertainty as to the molecular nature of the sealing mechanism and induced tissue bond. Further, energy-based techniques (e.g., tissue “fusion” or tissue “welding”) exhibit promise for the closure, repair and functional recovery ...


Modeling Liver Diseases Using Hepatic Cell Microarrays, Alexander David Roth Jan 2018

Modeling Liver Diseases Using Hepatic Cell Microarrays, Alexander David Roth

ETD Archive

Hepatocellular carcinoma (HCC) is an invasive and aggressive cancer of the liver that arises due to chronic cirrhosis. Research into understanding HCC has focused on two-dimensional (2D) and three-dimensional (3D) technologies to simulate the liver microenvironment and use animal models to model how HCC affects the rest of the body. 3D hydrogel models are desired because they can mimic the transport behavior observed in vivo by structurally mimicking the extracellular matrix (ECM) without the ethical concerns of animal models. However, hydrogels can be toxic to cells and require optimal procedures for appropriate handling. In this study, we created 3D models ...


The Role Of Protein-Protein Interactions In Inducing Interfacial Aggregation, Lea Line Gisele Sorret Jan 2018

The Role Of Protein-Protein Interactions In Inducing Interfacial Aggregation, Lea Line Gisele Sorret

Chemical & Biological Engineering Graduate Theses & Dissertations

The synergic exposure to silicone oil-water interfaces and to agitation has been shown to promote the aggregation of therapeutic proteins. Silicone oil is typically used as a lubricant for manufacturing surfaces and pharmaceutical containers such as glass prefilled syringes. This is a problem as protein aggregates formed at the silicone oil-water interface may shed into bulk solutions, leading to product recalls or adverse immunogenicity upon injections into patients.

Protein aggregation in solution occurs as a result of attractive protein-protein interactions (PPI). PPI can be characterized using techniques such as dynamic light scattering which measures the dependence of molecular diffusivity on ...


Modifying Supported Palladium Catalysts Using Self-Assembled Monolayers For Liquid-Phase Reactions, Pengxiao Hao Jan 2018

Modifying Supported Palladium Catalysts Using Self-Assembled Monolayers For Liquid-Phase Reactions, Pengxiao Hao

Chemical & Biological Engineering Graduate Theses & Dissertations

In this thesis, we investigate the modification of Pd/Al2O3 catalyst using thiolate and phosphonate self-assembled monolayers (SAMs) for two types of reactions in liquid-phase environments: selective oxidation of α, β-unsaturated alcohols and vanillin hydrodeoxygenation. Through the study, we aim to gain a better understanding of the liquid-solid interface that governs the catalytic activity.

The selective oxidation of a linear unsaturated alcohol trans-2-hexen-1-ol (HOL) was studied on Pd/Al2O3 catalysts in heptane. Alkanethiolate self-assembled monolayers (SAMs) were applied to modify Pd/Al2O3 catalysts; the sulfur content on the Pd surface was ...


Application Of Advances In Synthetic Biology To Modular Megasynthase Design And Scalable Combinatorial Biosynthesis, William Clifford Grau Jan 2018

Application Of Advances In Synthetic Biology To Modular Megasynthase Design And Scalable Combinatorial Biosynthesis, William Clifford Grau

Chemistry & Biochemistry Graduate Theses & Dissertations (1986-2018)

The diversity in structure and biological activity of natural products has long captured the imagination of chemists and molecular biologists alike. Natural products are synthesized by elaborate secondary metabolic pathways or enzymes. For example, modular megasynthases such as Type I Polyketide Synthases (PKSs), Non-ribosomal Peptide Synthetases (NRPSs), and PKS-NRPS hybrids, produce an array of quintessential natural products. The apparent modularity, defined architecture, and predictable chemistry of modular megasynthases make them especially attractive for combinatorial biosynthesis, which has long been a focus of biotechnology. Combinatorial biosynthetic libraries are of particular interest due to the high value of many natural products and ...


Connecting Protein Structure And Dynamics On Biomaterials With The Foreign Body Response, David Faulon Marruecos Jan 2018

Connecting Protein Structure And Dynamics On Biomaterials With The Foreign Body Response, David Faulon Marruecos

Chemical & Biological Engineering Graduate Theses & Dissertations

The harsh environment of the foreign body response (FBR) has the potential to negatively impact the implantations of biomaterials in the body. The FBR is initiated by inflammatory cells that recognize the material as foreign through surface-adsorbed proteins. When proteins interact with surfaces, they can unfold and expose epitopes that may be recognized by immune cells and trigger a series of reactions. Importantly, the presentation of unfolded proteins is directly influenced by the highly dynamic and heterogeneous behavior of proteins in near-surface environments, as well as by the physicochemical features of the underlying surface. Such behavior is the result of ...


Multicatalytic, Light-Driven Reactions For Improved Biofuels And Wastewater Remediation, Glenn Richard Hafenstine Jan 2018

Multicatalytic, Light-Driven Reactions For Improved Biofuels And Wastewater Remediation, Glenn Richard Hafenstine

Chemical & Biological Engineering Graduate Theses & Dissertations

Utilizing solar driven photocatalysis to facilitate coupling reaction systems is a promising route for low-energy and selective production of valuable chemicals. My doctoral research has focused on using photocatalytic cadmium sulfide nanorods to run novel reaction systems under mild, ambient conditions with sunlight as the only energy source. In this dissertation, I will first describe a cascade reaction system for catalytic upgrading of the fermentation products butanol and ethanol into an easily separated product in 2-ethylhexenal with a higher energy density. Specifically, I will report the use of the enzyme alcohol dehydrogenase, regeneration of the enzymatic cofactor by the photocatalyst ...


Engineering The Phospholipid Monolayer On Fluorocarbon, Hydrocarbon, And Liquid Crystal Nanodroplets For Applications In Biosensing, Rajarshi Chattaraj Jan 2018

Engineering The Phospholipid Monolayer On Fluorocarbon, Hydrocarbon, And Liquid Crystal Nanodroplets For Applications In Biosensing, Rajarshi Chattaraj

Mechanical Engineering Graduate Theses & Dissertations

Nanodroplets (NDs) are liquid-in-liquid dispersions of ~100-800 nm size range that are often stabilized by a shell of lipids, polymer, proteins, or surfactants. NDs have been explored for a variety of biomedical applications, mostly involving drug formulation and delivery. However, the unique properties of encapsulated liquids, and the effects of interfacial chemistry on these properties, makes NDs potentially powerful candidates for new biosensing technologies. This dissertation explores different oil-in-water or fluorocarbon-in-water ND systems for in-solution sensing of biomarkers as both a platform for diagnostic assays and as a precursor to in vivo biosensing. Nanodroplets, because of their size, provide a ...


Development Of Entubulation Strategies For Treating Central Nervous System Injuries, Ivy Brosch Jan 2018

Development Of Entubulation Strategies For Treating Central Nervous System Injuries, Ivy Brosch

Williams Honors College, Honors Research Projects

According to the McKnight Brain Institute, at least 10,000 people annually in the USA suffer from a central nervous system (CNS) injury. These injuries can cause serve disabilities including paralysis. Due to the complexity of the spinal cord, it is common that potential CNS treatments are first applied to an optic nerve crush (ONC) model in rats. Two proposed treatments were employed; one where nerve growth factor (NGF) was immobilized to a chitosan substrate to stimulate axonal regeneration, and the other using pentadecafluorooctanoyl chloride modified methacrylamide chitosan (MAC(Ali15)F) hydrogel to enhance local oxygenation. The two different treatments ...


Molded Features In Pdms For Fabricating Bacterial Cellulose For Various Geometries, Mitchell Habegger Jan 2018

Molded Features In Pdms For Fabricating Bacterial Cellulose For Various Geometries, Mitchell Habegger

Williams Honors College, Honors Research Projects

The purpose of producing features on bacterial cellulose (BC) is to facilitate the elongation and alignment for cells, in this case Normal Human Dermal Fibroblast (NHDF) cells. The elongated cells have applications in wound healing, tissue engineering, disease diagnostics, and many other fields. Experiments were run to test the effectiveness of transferring features to BC sheets from features induced by fracturing on polydimethylsiloxane (PDMS) and those duplicated from molds with existing features. The features were duplicated to BC sheets by either air drying or Guided Assembly-Based Biolithography (GAB). The research results showed that fracture inducing on PDMS produced very small ...


Surface Functionalization Via Photoinitiated Radical Polymerization For Rare Cell Isolation And Mechanical Protection, Calvin Frank Cahall Jan 2018

Surface Functionalization Via Photoinitiated Radical Polymerization For Rare Cell Isolation And Mechanical Protection, Calvin Frank Cahall

Theses and Dissertations--Chemical and Materials Engineering

Surface functionalization of living cells for cell therapeutics has gained substantial momentum in the last two decades. From encapsulating islets of Langerhans, to cell laden gels for tissue scaffolds, to individual cell encapsulation in thin hydrogels, to surface adhesives and inert surface camouflage, modification of living cell surfaces has a wide array of important applications. Here we use hydrogel encapsulation of individual cells as a mode of protection from mechanical forces for high throughput cell printing, and chemical stimuli for the isolation of rare cells in blood.

In the first study, we review methods of surface functionalization and establish a ...


High-Throughput Metabolism-Induced Toxicity Assays On A 384-Pillar Plate, Sooyeion Kang Jan 2018

High-Throughput Metabolism-Induced Toxicity Assays On A 384-Pillar Plate, Sooyeion Kang

ETD Archive

The U.S Environmental Protection Agency (EPA) launched the Transform Tox Testing Challenge in 2016 with the goal of developing practical methods that can be integrated into conventional high-throughput screening (HTS) assays to better predict the toxicity of parent compounds and their metabolites in vivo. In response to this need and to retrofit existing HTS assays for assessing metabolism-induced toxicity of compounds, we have developed a 384-pillar plate that is complementary to traditional 384-well plates and ideally suited for culturing human cells in three dimensions (3D) at a microscale. Briefly, human embryonic kidney (HEK) 293 cells in a mixture of ...