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Biomedical Engineering and Bioengineering Commons™
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Articles 1 - 25 of 25
Full-Text Articles in Biomedical Engineering and Bioengineering
Editorial: Intervertebral Disc Degeneration And Osteoarthritis: Mechanisms Of Disease And Functional Repair., Graciosa Q Teixeira, Jana Riegger, Raquel M Gonçalves, Makarand V. Risbud
Editorial: Intervertebral Disc Degeneration And Osteoarthritis: Mechanisms Of Disease And Functional Repair., Graciosa Q Teixeira, Jana Riegger, Raquel M Gonçalves, Makarand V. Risbud
Department of Orthopaedic Surgery Faculty Papers
No abstract provided.
Mechanical Properties And Morphological Alterations In Fiber-Based Scaffolds Affecting Tissue Engineering Outcomes, James Dolgin, Samerender Nagam Hanumantharao, Stephen Farias, Carl G. Simon, Smitha Rao
Mechanical Properties And Morphological Alterations In Fiber-Based Scaffolds Affecting Tissue Engineering Outcomes, James Dolgin, Samerender Nagam Hanumantharao, Stephen Farias, Carl G. Simon, Smitha Rao
Michigan Tech Publications
Electrospinning is a versatile tool used to produce highly customizable nonwoven nanofiber mats of various fiber diameters, pore sizes, and alignment. It is possible to create electrospun mats from synthetic polymers, biobased polymers, and combinations thereof. The post-processing of the end products can occur in many ways, such as cross-linking, enzyme linking, and thermal curing, to achieve enhanced chemical and physical properties. Such multi-factor tunability is very promising in applications such as tissue engineering, 3D organs/organoids, and cell differentiation. While the established methods involve the use of soluble small molecules, growth factors, stereolithography, and micro-patterning, electrospinning involves an inexpensive, labor …
Fibroblast-Generated Extracellular Matrix Guides Anastomosis During Wound Healing In An Engineered Lymphatic Skin Flap, Alvis Chiu, Wenkai Jia, Yumeng Sun, Jeremy Goldman, Feng Zhao
Fibroblast-Generated Extracellular Matrix Guides Anastomosis During Wound Healing In An Engineered Lymphatic Skin Flap, Alvis Chiu, Wenkai Jia, Yumeng Sun, Jeremy Goldman, Feng Zhao
Michigan Tech Publications
A healthy lymphatic system is required to return excess interstitial fluid back to the venous circulation. However, up to 49% of breast cancer survivors eventually develop breast cancer-related lymphedema due to lymphatic injuries from lymph node dissections or biopsies performed to treat cancer. While early-stage lymphedema can be ameliorated by manual lymph drainage, no cure exists for late-stage lymphedema when lymph vessels become completely dysfunctional. A viable late-stage treatment is the autotransplantation of functional lymphatic vessels. Here we report on a novel engineered lymphatic flap that may eventually replace the skin flaps used in vascularized lymph vessel transfers. The engineered …
Piezoelectric Signals In Vascularized Bone Regeneration, Delfo D’Alessandro, Claudio Ricci, Mario Milazzo, Giovanna Strangis, Francesca Forli, Gabriele Buda, Mario Petrini, Stefano Berrettini, M. Jasim Uddin, Serena Danti, Paolo Parchi
Piezoelectric Signals In Vascularized Bone Regeneration, Delfo D’Alessandro, Claudio Ricci, Mario Milazzo, Giovanna Strangis, Francesca Forli, Gabriele Buda, Mario Petrini, Stefano Berrettini, M. Jasim Uddin, Serena Danti, Paolo Parchi
Chemistry Faculty Publications and Presentations
The demand for bone substitutes is increasing in Western countries. Bone graft substitutes aim to provide reconstructive surgeons with off-the-shelf alternatives to the natural bone taken from humans or animal species. Under the tissue engineering paradigm, biomaterial scaffolds can be designed by incorporating bone stem cells to decrease the disadvantages of traditional tissue grafts. However, the effective clinical application of tissue-engineered bone is limited by insufficient neovascularization. As bone is a highly vascularized tissue, new strategies to promote both osteogenesis and vasculogenesis within the scaffolds need to be considered for a successful regeneration. It has been demonstrated that bone and …
Impact Of Angiogenic And Osteogenic Factors In The Presence Of Biodegradable Piezoelectric Films, Jayla Millender
Impact Of Angiogenic And Osteogenic Factors In The Presence Of Biodegradable Piezoelectric Films, Jayla Millender
University Scholar Projects
One of the most common causes of bone graft rejection is lack of a vascular network connecting the graft to the existing native tissue – allowing for nutrient flow. Under current grafting techniques, the existing blood vessel network in the patient slowly invades the implant in order to supply the injured site with its necessary nutrients. The purpose of this research is to determine if a synthetic bone graft with a stable microvascular network can be developed in vitro. I hypothesize that the use of indirect angiogenic factors such as sonic hedgehog homolog and hypoxia-inducible factor-1 in combination with the …
Impact Of Angiogenic And Osteogenic Factors In The Presence Of Biodegradable Piezoelectric Films, Jayla Millender
Impact Of Angiogenic And Osteogenic Factors In The Presence Of Biodegradable Piezoelectric Films, Jayla Millender
Honors Scholar Theses
One of the most common causes of bone graft rejection is lack of a vascular network connecting the graft to the existing native tissue – allowing for nutrient flow. Under current grafting techniques, the existing blood vessel network in the patient slowly invades the implant in order to supply the injured site with its necessary nutrients. The purpose of this research is to determine if a synthetic bone graft with a stable microvascular network can be developed in vitro. I hypothesize that the use of indirect angiogenic factors such as sonic hedgehog homolog and hypoxia-inducible factor-1 in combination with the …
Electroactive Polymeric Composites To Mimic The Electromechanical Properties Of Myocardium In Cardiac Tissue Repair, Kaylee Meyers, Bruce Lee, Rupak Rajachar
Electroactive Polymeric Composites To Mimic The Electromechanical Properties Of Myocardium In Cardiac Tissue Repair, Kaylee Meyers, Bruce Lee, Rupak Rajachar
Michigan Tech Publications
Due to the limited regenerative capabilities of cardiomyocytes, incidents of myocardial infarction can cause permanent damage to native myocardium through the formation of acellular, non-conductive scar tissue during wound repair. The generation of scar tissue in the myocardium compromises the biomechanical and electrical properties of the heart which can lead to further cardiac problems including heart failure. Currently, patients suffering from cardiac failure due to scarring undergo transplantation but limited donor availability and complications (i.e., rejection or infectious pathogens) exclude many individuals from successful transplant. Polymeric tissue engineering scaffolds provide an alternative approach to restore normal myocardium structure and function …
Two-Dimensional & Three-Dimensional Microarray Cell Culture Using Elastomeric Assembly Substrates, Angel Olivera-Torres
Two-Dimensional & Three-Dimensional Microarray Cell Culture Using Elastomeric Assembly Substrates, Angel Olivera-Torres
Honors Theses
Tissue engineering and regenerative medicine represent the collection of all engineering disciplines brought together for the common goal of developing novel ways of growing tissues and organs in the laboratory. Efforts have made it possible to replicate or induce growth of 2D structures in the human body like skin, but the clinical need for on-demand solid organs has yet to be met due to lack of understanding of the variables responsible for organogenesis. Cell-colony heterogeneity, 3D-cellular architecture, bioactive molecules, and crosstalk communication between parenchymal cell populations need to be further investigated, and high-throughput technologies can rapidly increase the rate at …
The Use Of Tissue Engineering To Fabricate Perfusable 3d Brain Microvessels, Kalpani N Udeni Galpayage Dona, Jonathan Franklin Hale, Tobi Salako, Akanksha Anandanatarajan, Kiet A Tran, Brandon J Deore, Peter Galie, Servio Heybert Ramirez, Allison Michelle Andrews
The Use Of Tissue Engineering To Fabricate Perfusable 3d Brain Microvessels, Kalpani N Udeni Galpayage Dona, Jonathan Franklin Hale, Tobi Salako, Akanksha Anandanatarajan, Kiet A Tran, Brandon J Deore, Peter Galie, Servio Heybert Ramirez, Allison Michelle Andrews
Henry M. Rowan College of Engineering Faculty Scholarship
Tissue engineering of the blood-brain barrier (BBB) in vitro has been rapidly expanding to address the challenges of mimicking the native structure and function of the BBB. Most of these models utilize 2D conventional microfluidic techniques. However, 3D microvascular models offer the potential to more closely recapitulate the cytoarchitecture and multicellular arrangement of in vivo microvasculature, and also can recreate branching and network topologies of the vascular bed. In this perspective, we discuss current 3D brain microvessel modeling techniques including templating, printing, and self-assembling capillary networks. Furthermore, we address the use of biological matrices and fluid dynamics. Finally, key challenges …
Consistent And Reproducible Cultures Of Large-Scale 3d Mammary Epithelial Structures Using An Accessible Bioprinting Platform, John A. Reid, Peter M. Mollica, Robert D. Bruno, Patrick C. Sachs
Consistent And Reproducible Cultures Of Large-Scale 3d Mammary Epithelial Structures Using An Accessible Bioprinting Platform, John A. Reid, Peter M. Mollica, Robert D. Bruno, Patrick C. Sachs
Medical Diagnostics & Translational Sciences Faculty Publications
Background: Standard three-dimensional (3D) in vitro culture techniques, such as those used for mammary epithelial cells, rely on random distribution of cells within hydrogels. Although these systems offer advantages over traditional 2D models, limitations persist owing to the lack of control over cellular placement within the hydrogel. This results in experimental inconsistencies and random organoid morphology. Robust, high-throughput experimentation requires greater standardization of 3D epithelial culture techniques.
Methods: Here, we detail the use of a 3D bioprinting platform as an investigative tool to control the 3D formation of organoids through the "self-assembly" of human mammary epithelial cells. Experimental bioprinting procedures …
Application Of Composite Hydrogels To Control Physical Properties In Tissue Engineering And Regenerative Medicine, Cassidy Sheffield, Kaylee Meyers, Emil Johnson, Rupak Rajachar
Application Of Composite Hydrogels To Control Physical Properties In Tissue Engineering And Regenerative Medicine, Cassidy Sheffield, Kaylee Meyers, Emil Johnson, Rupak Rajachar
Michigan Tech Publications
The development of biomaterials for the restoration of the normal tissue structure–function relationship in pathological conditions as well as acute and chronic injury is an area of intense investigation. More recently, the use of tailored or composite hydrogels for tissue engineering and regenerative medicine has sought to bridge the gap between natural tissues and applied biomaterials more clearly. By applying traditional concepts in engineering composites, these hydrogels represent hierarchical structured materials that translate more closely the key guiding principles required for improved recovery of tissue architecture and functional behavior, including physical, mass transport, and biological properties. For tissue-engineering scaffolds in …
Recent Developments In Tough Hydrogels For Biomedical Applications, Yuan Liu, Weilue He, Zhongtian Zhang, Bruce P. Lee
Recent Developments In Tough Hydrogels For Biomedical Applications, Yuan Liu, Weilue He, Zhongtian Zhang, Bruce P. Lee
Department of Biomedical Engineering Publications
A hydrogel is a three-dimensional polymer network with high water content and has been attractive for many biomedical applications due to its excellent biocompatibility. However, classic hydrogels are mechanically weak and unsuitable for most physiological load-bearing situations. Thus, the development of tough hydrogels used in the biomedical field becomes critical. This work reviews various strategies to fabricate tough hydrogels with the introduction of non-covalent bonds and the construction of stretchable polymer networks and interpenetrated networks, such as the so-called double-network hydrogel. Additionally, the design of tough hydrogels for tissue adhesive, tissue engineering, and soft actuators is reviewed.
3d Tissue Engineering, An Emerging Technique For Pharmaceutical Research, Gregory Jensen, Christian Morrill, Yu Huang
3d Tissue Engineering, An Emerging Technique For Pharmaceutical Research, Gregory Jensen, Christian Morrill, Yu Huang
Biological Engineering Faculty Publications
Tissue engineering and the tissue engineering model have shown promise in improving methods of drug delivery, drug action, and drug discovery in pharmaceutical research for the attenuation of the central nervous system inflammatory response. Such inflammation contributes to the lack of regenerative ability of neural cells, as well as the temporary and permanent loss of function associated with neurodegenerative diseases such as Alzheimer's disease, Parkinson's disease, and traumatic brain injury. This review is focused specifically on the recent advances in the tissue engineering model made by altering scaffold biophysical and biochemical properties for use in the treatment of neurodegenerative diseases. …
Patient-Derived Hydrogel As A Sacrificial Matrix For Efficient Cell Loading, Shalmli U. Joshi
Patient-Derived Hydrogel As A Sacrificial Matrix For Efficient Cell Loading, Shalmli U. Joshi
Honors Scholar Theses
The field of tissue engineering focuses on delivering patient-derived stem cells to the body through the use of degradable biomaterials, such as hydrogels, which are infused into engineered scaffolds. Hydrogels act as templates to support and guide cells towards the regeneration of new tissue. In this study, we introduce a completely intraoperative procedure for obtaining a fibrin hydrogel for tissue engineering applications. In the past, fibrin hydrogel has been commonly formed by combining fibrinogen protein with animal-derived thrombin. Instead, we have developed an automated, and therefore reproducible, protocol to isolate and form fibrin hydrogel without the use of animal-derived thrombin. …
Accessible Bioprinting: Adaptation Of A Low-Cost 3d-Printer For Precise Cell Placement And Stem Cell Differentiation, John A. Reid, Peter A. Mollica, Garett D. Johnson, Roy C. Ogle, Robert D. Bruno, Patrick C. Sachs
Accessible Bioprinting: Adaptation Of A Low-Cost 3d-Printer For Precise Cell Placement And Stem Cell Differentiation, John A. Reid, Peter A. Mollica, Garett D. Johnson, Roy C. Ogle, Robert D. Bruno, Patrick C. Sachs
Medical Diagnostics & Translational Sciences Faculty Publications
The precision and repeatability offered by computer-aided design and computer-numerically controlled techniques in biofabrication processes is quickly becoming an industry standard. However, many hurdles still exist before these techniques can be used in research laboratories for cellular and molecular biology applications. Extrusion-based bioprinting systems have been characterized by high development costs, injector clogging, difficulty achieving small cell number deposits, decreased cell viability, and altered cell function post-printing. To circumvent the high-price barrier to entry of conventional bioprinters, we designed and 3D printed components for the adaptation of an inexpensive 'off-the-shelf' commercially available 3D printer. We also demonstrate via goal based …
When 1 + 1 > 2: Nanostructured Composites For Hard Tissue Engineering Applications, Vuk Uskoković
When 1 + 1 > 2: Nanostructured Composites For Hard Tissue Engineering Applications, Vuk Uskoković
Pharmacy Faculty Articles and Research
Multicomponent, synergistic and multifunctional nanostructures have taken over the spotlight in the realm of biomedical nanotechnologies. The most prospective materials for bone regeneration today are almost exclusively composites comprising two or more components that compensate for the shortcomings of each one of them alone. This is quite natural in view of the fact that all hard tissues in the human body, except perhaps the tooth enamel, are composite nanostructures. This review article highlights some of the most prospective breakthroughs made in this research direction, with the hard tissues in main focus being those comprising bone, tooth cementum, dentin and enamel. …
Cellular Events Under Flow States Pertinent To Heart Valve Function, Glenda L. Castellanos
Cellular Events Under Flow States Pertinent To Heart Valve Function, Glenda L. Castellanos
FIU Electronic Theses and Dissertations
Heart valve disease (HVD) or a damaged valve can severely compromise the heart's ability to pump efficiently. Balloon valvuloplasty is preferred on neonates with aortic valve stenosis. Even though this procedure decreases the gradient pressure across the aortic valve, restenosis is observed soon after balloon intervention. Tissue engineering heart valves (TEHV), using bone marrow stem cells (BMSCs) and biodegradable scaffolds, have been investigated as an alternative to current non-viable prosthesis. By observing the changes in hemodynamics following balloon aortic valvuloplasty, we could uncover a potential cause for rapid restenosis after balloon intervention. Subsequently, a tissue engineering treatment strategy based on …
Design And Development Of Two Component Hydrogel Ejector For Three-Dimensional Cell Growth, Thomas Dunkle, Jessica Deschamps, Connie Dam
Design And Development Of Two Component Hydrogel Ejector For Three-Dimensional Cell Growth, Thomas Dunkle, Jessica Deschamps, Connie Dam
Honors Scholar Theses
Hydrogels are useful in wound healing, drug delivery, and tissue engineering applications, but the available methods of injecting them quickly and noninvasively are limited. The medical industry does not yet have access to an all-purpose device that can quickly synthesize hydrogels of different shapes and sizes. Many synthesis procedures that have been developed result in the formation of amorphous hydrogels. While generally useful, amorphous hydrogels exhibit limited capability in tissue engineering applications, especially due to their viscous properties. This endeavor aims to modulate the appropriate gelation parameters, optimize the injection process, and create a prototype that allows for the extrusion …
Finite Element Analysis Of The Application Of Ultrasound-Generated Acoustic Radiation Force To Biomaterials, Nicole J. Piscopo
Finite Element Analysis Of The Application Of Ultrasound-Generated Acoustic Radiation Force To Biomaterials, Nicole J. Piscopo
Honors Scholar Theses
While most bone fractures can heal simply by being stabilized, others can take a longer time to rejoin or they could fail to merge back together completely. Numerous studies have shown the positive effects that ultrasonic therapy have had on delayed-union and non-union bone fracture repair but little is known as to what specific biological mechanisms are at play. Ultrasound may be a valuable tool for bone tissue regeneration at these fracture sites using a tissue engineering approach, however, more must be understood about its impact on stimulating tissues to heal before this can be a reality. For that reason, …
Fluid Flow-Induced Mesenchymal Stem Cell Migration: Role Of Fak And Rock Mechanosensors, Brandon D. Riehl
Fluid Flow-Induced Mesenchymal Stem Cell Migration: Role Of Fak And Rock Mechanosensors, Brandon D. Riehl
Department of Mechanical and Materials Engineering: Dissertations, Theses, and Student Research
The study of mesenchymal stem cell (MSC) migration under mechanical stimulation conditions with investigation of the underlying molecular mechanism could lead to a better understanding and outcomes in stem cell-based regenerative medicine. MSCs having multipotent regenerative capability exist in niches in the bone marrow, muscle, vasculature, and in other tissues throughout the body, and their migration through tissues and vasculature for the repair of damaged tissue is a key process of cell and tissue homeostasis, remodeling, and regeneration. While cell migration in response to cytokines and other chemo-attractants is relatively well understood, little is revealed in regard to the effect …
Modeling And Experimental Methods To Predict Oxygen Distribution In Bone Defects Following Cell Transplantation, Christopher M. Heylman, Sharon Santoso, Melissa D. Krebs, Gerald M. Saidel, Eben Alsberg, George F. Muschler
Modeling And Experimental Methods To Predict Oxygen Distribution In Bone Defects Following Cell Transplantation, Christopher M. Heylman, Sharon Santoso, Melissa D. Krebs, Gerald M. Saidel, Eben Alsberg, George F. Muschler
Biomedical Engineering
We have developed a mathematical model that allows simulation of oxygen distribution in a bone defect as a tool to explore the likely effects of local changes in cell concentration, defect size or geometry, local oxygen delivery with oxygen-generating biomaterials (OGBs), and changes in the rate of oxygen consumption by cells within a defect. Experimental data for the oxygen release rate from an OGB and the oxygen consumption rate of a transplanted cell population are incorporated into the model. With these data, model simulations allow prediction of spatiotemporal oxygen concentration within a given defect and the sensitivity of oxygen tension …
Stem Cell Based Tissue Engineering And Regenerative Medicine: A Review Focusing On Adult Stem Cells, Jordan Greenberg, Veronica R. Fortino, Daniel Pelaez, Herman S. Cheung
Stem Cell Based Tissue Engineering And Regenerative Medicine: A Review Focusing On Adult Stem Cells, Jordan Greenberg, Veronica R. Fortino, Daniel Pelaez, Herman S. Cheung
Biology Faculty Articles
Tissue engineering has emerged as a field that attempts to harness the bodies' own developmental and repair features to treat diseases and illnesses. Many of these illnesses are caused by necrosis or loss of functionality of complete organs or specific cell types. Early discoveries in embryonic stem cells fueled a wave of research that led to claims about possibly regenerating nonfunctioning organs. Although we are still far away from being able to grow functional organs in a Petri dish, the field continues to progress forward, and new clinical trials have been approved for using both embryonic and adult stem cell …
Investigation Of A New Material For Heart Valve Tissue Engineering, Claire Brougham, Nian Shen, Allison Cudsworth, Thomas Flanagan, Stefan Jockenhoevel, Fergal O'Brien
Investigation Of A New Material For Heart Valve Tissue Engineering, Claire Brougham, Nian Shen, Allison Cudsworth, Thomas Flanagan, Stefan Jockenhoevel, Fergal O'Brien
Conference Papers
No abstract provided.
Magic From Human Regenerative Technologies -- Stem Cells, John M. Ritz
Magic From Human Regenerative Technologies -- Stem Cells, John M. Ritz
STEMPS Faculty Publications
The article discusses the developments in stem cell research and development. It mentions that through tissue engineering or regenerative medicine, many diseases are finding techniques for improvement and cure. It discusses the role of stem cells in mending the human body and the ethics associated with this technology . It is mentioned that stem cells are divided in 2 types: embryonic stem cells and adult stem cells. It includes the process of cell differentiation which determines the properties and characteristics of cells. It mentions several diseases related to sports injury, spinal cord injuries, blood and heart disorders to be to …
Controlled Release Systems For Dna Delivery, Angela K. Pannier, Lonnie D Shea
Controlled Release Systems For Dna Delivery, Angela K. Pannier, Lonnie D Shea
Biological Systems Engineering: Papers and Publications
Adapting controlled release technologies to the delivery of DNA has the potential to overcome extracellular barriers that limit gene therapy. Controlled release systems can enhance gene delivery and increase the extent and duration of transgene expression relative to more traditional delivery methods (e.g., injection). These systems typically deliver vectors locally, which can avoid distribution to distant tissues, decrease toxicity to nontarget cells, and reduce the immune response to the vector. Delivery vehicles for controlled release are fabricated from natural and synthetic polymers, which function either by releasing the vector into the local tissue environment or by maintaining the vector at …