Biomedical Engineering and Bioengineering Commons^™

Influence Of Isoflurane On Left Atrial Function In Dogs With Pacing-Induced Cardiomyopathy: Evaluation With Pressure-Volume Relationships, Franz Kehl, John F. Ladisa, Douglas Anthony Hettrick, Judy R. Kersten, David C. Warltier, Paul S. Pagel

Biomedical Engineering Faculty Research and Publications

Objective

The actions of volatile anesthetics on left ventricular (LV) function in normal and failing hearts have been previously evaluated, but the effects of these agents on left atrial (LA) function in the presence of LV dysfunction are unknown. The hypothesis was tested that isoflurane alters LA mechanics evaluated with pressure-volume relations.

Design

Prospective.

Setting

Laboratory.

Participants

Barbiturate-anesthetized dogs (n = 8) were instrumented for measurement of aortic, LA, and LV pressures (micromanometers), and LA volume (epicardial orthogonal sonomicrometers) after 3 weeks of rapid ventricular pacing (220 beats/min).

Interventions

LA myocardial contractility (E_es) was assessed with end-systolic pressure-volume …

The Stromal Cell-Derived Factor-1alpha/Cxcr4 Ligand-Receptor Axis Is Critical For Progenitor Survival And Migration In The Pancreas., Ayse G. Kayali, Kurt Van Gunst, Iain L. Campbell, Aleksandr Stotland, Marcie Kritzik, Guoxun Liu, Malin Flodström-Tullberg, You-Qing Zhang, Nora Sarvetnick

Journal Articles: Regenerative Medicine

The SDF-1alpha/CXCR4 ligand/chemokine receptor pair is required for appropriate patterning during ontogeny and stimulates the growth and differentiation of critical cell types. Here, we demonstrate SDF-1alpha and CXCR4 expression in fetal pancreas. We have found that SDF-1alpha and its receptor CXCR4 are expressed in islets, also CXCR4 is expressed in and around the proliferating duct epithelium of the regenerating pancreas of the interferon (IFN) gamma-nonobese diabetic mouse. We show that SDF-1alpha stimulates the phosphorylation of Akt, mitogen-activated protein kinase, and Src in pancreatic duct cells. Furthermore, migration assays indicate a stimulatory effect of SDF-1alpha on ductal cell migration. Importantly, blocking …

Tongue Movements In Feeding And Speech, Karen M. Hiiemae, Jeffrey B. Palmer

Biomedical and Chemical Engineering - All Scholarship

The position of the tongue relative to the upper and lower jaws is regulated in part by the position of the hyoid bone, which, with the anterior and posterior suprahyoid muscles, controls the angulation and length of the floor of the mouth on which the tongue body 'rides'. The instantaneous shape of the tongue is controlled by the 'extrinsic muscles ' acting in concert with the 'intrinsic ' muscles. Recent anatomical research in non-human mammals has shown that the intrinsic muscles can best be regarded as a 'laminated segmental system ' with tightly packed layers of the 'transverse', 'longitudinal', and …

Preliminary Work On The Antibacterial Effect Of Strontium In Glass Ionomer Cements, A. Guida, Mark R. Towler, J. G. Wall, R. G. Hill, S. Eramo

Chemical and Biochemical Engineering Faculty Research & Creative Works

The antibacterial effect of strontium in glass ionomer cements (GIC) was discussed. The glass transition temperatures were found to decrease with the increasing fluorine content consistent with the fluorine replacing bridging oxygens. However no significant correlation was found between the fluoride release and the antibacterial activity.

Interpreting Hemoglobin And Water Concentration, Oxygen Saturation, And Scattering Measured In Vivo By Near-Infrared Breast Tomography, Subhadra Srinivasan, Brian W. Pogue, Shudong Jiang, Hamid Dehghani, Christine Kogel, Sandra Soho, Jennifer J. Gibson, Tor D. Tosteson, Steven P. Poplack, Keith D. Paulsen

Dartmouth Scholarship

Near-infrared spectroscopic tomography was used to measure the properties of 24 mammographically normal breasts to quantify whole-breast absorption and scattering spectra and to evaluate which tissue composition characteristics can be determined from these spectra. The absorption spectrum of breast tissue allows quantification of (i) total hemoglobin concentration, (ii) hemoglobin oxygen saturation, and (iii) water concentration, whereas the scattering spectrum provides information about the size and number density of cellular components and structural matrix elements. These property data were tested for correlation to demographic information, including subject age, body mass index, breast size, and radiographic …

Development Of Standard Methods To Estimate Manure Production And Nutrient Characteristics From Dairy Cattle, T. Nennich, J. H. Harrison, D. Meyer, W. P. Weiss, A. J. Heinrichs, R. L. Kincaid, W. J. Powers, Richard K. Koelsch, P. E. Wright

Department of Biological Systems Engineering: Conference Presentations and White Papers

Total collection data from many universities were pooled for statistical analyses to evaluate existing data from dairy animals and determine if one or more modifications were needed for Standard Table D384.1. Many of these studies were carried out to evaluate nutritional characteristics associated with different diets. The data collected were sufficient to evaluate total manure, total and volatile solids, and N excretion values. Some experiments had sufficient number of samples analyzed to evaluate P, K, Ca, Mg, Na, Cl, S, and micro-elements. Statistical analyses were conducted to determine if a single column value was appropriate and define the regression equations …

Proposed Beef Cattle Manure Excretion And Characteristics Standard For Asae, Galen E. Erickson, B. Auvermann, R. A. Eigenberg, L. W. Greene, Terry J. Klopfenstein, Richard K. Koelsch

Department of Biological Systems Engineering: Conference Presentations and White Papers

A committee was formed consisting of both animal scientists and agricultural engineers to evaluate and update current ASAE standards. An intake minus retention model was developed to estimate nutrient excretion. This approach allows users (producers, engineers, etc.) to develop site specific information based on known variables such as protein or phosphorus content of diets and cattle performance. This approach illustrates the importance of nutrition on nutrient excretion in livestock operations. Our focus is for feedlot cattle and updates excretion of dry matter (DM or total solids), organic matter (OM or volatile solids), N, P, Ca, K, Na, Mg, S, Cu, …

Use Of Computer Spreadsheets And Paper-Based Workbooks To Teach Comprehensive Nutrient Management Planning, Charles A. Shapiro, R. L. Deloughery, Richard K. Koelsch, M. J. Kucera, Charles S. Wortmann

Department of Biological Systems Engineering: Conference Presentations and White Papers

The objective of this program was to provide producers and ag advisors the skills needed to apply for and maintain the non-engineering components of a Nebraska permit to operate a livestock waste control facility. This includes a Comprehensive Nutrient Management Plan (CNMP). Eighty six producers and advisors participated in an intensive three-session hands-on class with a total of nine contact hours at four sites in eastern Nebraska in the spring of 2002. The workshop included lecture and participant use of spreadsheet tools to assist with performing the necessary calculations. With an average of 68 % responses per session, 58% rated …

Neural Electronic Signal Detection Fidelity And Membrane Conductance, Robert B. Szlavik

Biomedical Engineering

Abstract of paper presented at conference.

Phosphorus Management, Bahman Eghball, J.F. Shanahan, Gary E. Varvel, John E. Gilley

Biological Systems Engineering: Papers and Publications

Soils with high levels of P can contribute to excess P in runoff and subsequently pollute the surface water. Excess P in the soil can be removed from the system by harvesting crops. The objectives of this study were to evaluate corn (Zea mays L.) P removal effects on soil P reduction, and to evaluate various corn hybrids and soybean [Glycine max (L.) Merr.] varieties for differences in grain P concentration and P removal. Soil with varying P levels as a result of annual or biennial beef cattle (Bos Taurus) feedlot manure or compost application was …

Use Of Computer Spreadsheets And Paper-Based Workbooks To Teach Comprehensive Nutrient Management Planning, Charles A. Shapiro, R. L. Deloughery, Richard K. Koelsch, M. K. Kucera, Charles S. Wortmann

Biological Systems Engineering: Papers and Publications

The objective of this program was to provide producers and ag advisors the skills needed to apply for and maintain the non-engineering components of a Nebraska permit to operate a livestock waste control facility. This includes a Comprehensive Nutrient Management Plan (CNMP). Eighty six producers and advisors participated in an intensive three-session hands-on class with a total of nine contact hours at four sites in eastern Nebraska in the spring of 2002. The workshop included lecture and participant use of spreadsheet tools to assist with performing the necessary calculations. With an average of 68 % responses per session, 58% rated …

Fact Sheet #2: Do I Need A Npdes Permit For My Livestock Or Poultry Operation, Richard K. Koelsch

Department of Biological Systems Engineering: Conference Presentations and White Papers

New U.S. Environmental Protection Agency (EPA) rules that took effect April 14, 2003 indicate that animal feeding operations (AFOs) can be defined as concentrated animal feeding operations (CAFOs) and required to obtain a National Pollutant Discharge Elimination System (NPDES) permit from the EPA or a designated state permitting authority. The likelihood of an AFO being classified as a CAFO will depend, in large part, on whether that operation is considered to be large, medium, or small.

A Concept For Early Cancer Detection And Therapy, Ronald W. Waynant, Ilko K. Ilev, Kunal Mitra

Biomedical Engineering and Sciences Faculty Publications

Early detection and treatment of breast cancer is least costly in terms of dollars, morbidity and mortality. With new early detection x-ray technology, tumors can be found, diagnosed and treated at a much smaller size than is currently possible. This paper proposes the development of a high resolution, high quality imaging system. It is a laser-driven x-ray system with time-gated detection that removes scattering noise in the image and produces resolution on the order of 10 μm. This higher resolution and higher image quality will enable the detection of one or two millimeter tumors hopefully detecting them before metastasis. We …

Characteristics Of Phototrophic And Non-Phototrophic Lagoons For Swine Manure, Tongtong Chen, Dennis D. Schulte, Richard K. Koelsch, Anne M. Parkhurst

Biological Systems Engineering: Papers and Publications

Odors are a major result of inadequately sized and mismanaged anaerobic lagoons. However, purple or pink colored lagoons, indicating the presence of phototrophic purple bacteria, are less likely to be an odor nuisance than are non–purple lagoons. Eight swine lagoons were studied to quantify critical parameters thought to allow purple lagoons to be a more reliable odor control alternative. Bacteriochlorophyll a (Bchl a), which indirectly measures the abundance of phototrophic bacteria, was greater in purple lagoons than in non–purple lagoons (P = 0.01). Oxidation–reduction potential (ORP) was less negative for purple lagoons than for non–purple lagoons in both spring …

Local Applications Of The National Livestock And Poultry Environmental Stewardship Curriculum, Diane Huntrods, Richard K. Koelsch, Frank Humenik

Department of Biological Systems Engineering: Conference Presentations and White Papers

This paper introduces the LPES curriculum project, summarizing the curriculum development process, the final curriculum materials, and the outreach activities. Following an overview of the LPES Curriculum Impact Survey, the authors will present representative examples of uses and modifications made of the LPES curriculum as it is used with targeted audiences. This model for utilizing national expertise to address state and local educational needs may have benefit in other extension education programs as land grant university resources decrease. The paper concludes with a discussion of lessons learned from this national extension education curriculum development effort.

A Computational Method For Internal Radiation Dosimetry At The Voxel Level, Swapna Chigurupati

FIU Electronic Theses and Dissertations

The current development of hybrid SPECT/CT and PET/CT systems allows not only accurate attenuation correction of images, but also provide an anatomical frame for the 3D spatial representation of the dose distribution. The main goal of this thesis project was to develop computational algorithms for calculation of the 3D dose distribution delivered by internal emitters based on the images and information provided by new hybrid SPECT/CT and PET/CT systems. Although many experimental problems exist in patient-specific dosimetry, current drawback is the lack of radionuclide voxel S values for the set of all possible combinations of cubical and non-cubical pixel edges …

Accuracy Of Activity Quantitation Of F-18 Fluorodeoxyglucose (Fdg) Positron Emission Tomography (Pet) Imaging Using Simulated Malignant Tumors, Madhu Durai

FIU Electronic Theses and Dissertations

This thesis involves a procedure, which calculated and compared the sum of all the pixel counts, threshold pixel counts sum of a 3D PET image and mean and maximum pixel count of one single transaxial slice (2D) of simulated tumors for a chosen region of interest (ROI). A calibration factor was multiplied by the sum of the pixel counts, threshold pixel counts sum of all the transaxial slices, and the mean, and maximum pixel counts of one single transaxial slice in an ROI to calculate for the activity of the tumor. This activity calculated was compared with the real activity …

A Dosimetry And Radiobiological Model For Intravascular Brachytherapy Treatment Planning With Radioisotope Emitting Stents, Maria Alejandra Caceres

FIU Electronic Theses and Dissertations

The aim of this study was to develop a practical, versatile and fast dosimetry and radiobiological model for calculation of the 3D dose distribution and radiobiological effectiveness of radioactive stents. The algorithm was written in Matlab 6.5 programming language and is based on the dose point kernel convolution. The dosimetry and radiobiological model was applied for evaluation of the 3D dose distribution of ³²P, ⁹⁰Y, ¹⁸⁸Re and ¹⁷⁷Lu stents. Of the four, ³²P delivers the highest dose, while ⁹⁰Y, ¹⁸⁸Re and ¹⁷⁷Lu require high levels of activity to deliver a significant therapeutic …

Short Pulse Laser Propagation Through Tissues, Champak Das, Ashish Trivedi, Kunal Mitra, Tuan Vo-Dinh

Biomedical Engineering and Sciences Faculty Publications

An experimental and numerical study is performed to analyze short pulse laser propagation through tissue phantoms without and with inhomogeneities/tumors imbedded in it. Short pulse laser probing techniques has distinct advantages over conventional very large pulse width or cw lasers primarily due to the additional information conveyed about the tissue interior by the temporal variation of the observed signal. Both the scattered temporal transmitted and reflected optical signals are measured experimentally using a streak camera for samples irradiated with a short pulse laser source. Parametric study involving different scattering and absorption coefficients of tissue phantoms and inhomogeneities as well as …

Static And Dynamic Mechanical Testing Of A Polymer With Potential Use As Heart Valve Material, Andres Felipe Aguirre

FIU Electronic Theses and Dissertations

Synthetic tri-leaflet heart valves generally fail in the long-term use (more than 10 years). Tearing and calcification of the leaflets usually cause failure of these valves as a consequence of high tensile and bending stresses borne on the material. The primary purpose of this study was to explore the possibilities of a new polymer composite to be used as synthetic tri-leaflet heart valve material. This composite was comprised of polystyrene-polyisobutylene-polystyrene (Quatromer), a proprietary polymer, embedded with continuous polypropylene (PP) fibers. Quatromer had been found to be less likely to degrade in vivo than polyurethane. Moreover, it was postulated that a …

Development Of An Atrophic Nonunion Model And Comparison To A Closed Healing Fracture In Rat Femur, Takeshi Kokubu, David J. Hak, Scott J. Hazelwood, A. Hari Reddi

Biomedical Engineering

Although most fractures heal, some fail to heal and become nonunions. Many animal models have been developed to study problems of fracture healing. The majority of nonunion models have involved segmental bone defects, but this may not adequately represent the biologic condition in which nonunions clinically develop. The objective of the present study is to develop a nonunion model that better simulates the clinical situation in which there is soft tissue damage including periosteal disruption and to compare this model to a standard closed fracture model utilizing identical fracture stabilization, providing a similar mechanical environment. A total of 96 three …

Osteon Pullout In The Equine Third Metacarpal Bone: Effects Of Ex Vivo Fatigue, L. P. Hiller, S. M. Stover, V. A. Gibson, J. C. Gibeling, C. S. Prater, S. J. Hazelwood, O. C. Yeh, R. B. Martin

Biomedical Engineering

An important concept in bone mechanics is that osteons influence mechanical properties in several ways, including contributing to toughness and fatigue strength by debonding from the interstitial matrix so as to "bridge" developing cracks. Observations of "pulled out" osteons on fracture surfaces are thought to be indicative of such behavior. We tested the hypothesis that osteon pullout varies with mode of loading (fatigue vs. monotonic), cortical region, elastic modulus, and fatigue life. Mid-diaphseal beams from the dorsal, medial, and lateral regions of the equine third metacarpal bone were fractured in four point bending by monotonic loading to failure under deflection …

Reactive Oxygen Species Modulate Coronary Wall Shear Stress And Endothelial Function During Hyperglycemia, Eric R. Gross, John F. Ladisa, Dorothee Weihrauch, Lars E. Olson, Tobias T. Kress, Douglas Anthony Hettrick, Paul S. Pagel, David C. Warltier, Judy R. Kersten

Biomedical Engineering Faculty Research and Publications

Hyperglycemia is associated with generation of reactive oxygen species (ROS), and this action may contribute to accelerated atherogenesis. We tested the hypothesis that hyperglycemia produces alterations in left anterior descending coronary artery (LAD) wall shear stress concomitant with endothelial dysfunction and ROS production in dogs (n = 12) instrumented for measurement of LAD blood flow, velocity, and diameter. Dogs were randomly assigned to receive vehicle (0.9% saline) or the superoxide dismutase mimetic 4- hydroxy-2,2,6,6-tetramethylpiperidine-1-oxyl (tempol) and were administered intravenous infusions of d-glucose to achieve target blood glucose concentrations of 350 and 600 mg/dl (moderate and severe hyperglycemia, respectively). Endothelial …

Investigation Into The Ultrasound Setting Of Glass Ionomer Cements. Part I. Postulated Modalities, Mark R. Towler, C. M. Crowley, R. G. Hill

Chemical and Biochemical Engineering Faculty Research & Creative Works

Fuji IX glass is a low phosphate, low soda containing glass which has strontia incorporated as a radio-pacifier. This glass material was analyzed during exposure to different durations of ultrasound in an attempt to determine how the glass phase reacts. Cross sections of Fuji IX cement, set both conventionally and by ultrasound, were subsequently compared by microscopy. The resultant data were analyzed in detail.

Adenosine Type 1 (A ) Receptors Mediate Protection Against Myocardial 1 Infarction Produced By Chronic, Intermittent Ingestion Of Ethanol In Dogs, Franz Kehl, John G. Krolikowski, John F. Ladisa, Judy R. Kersten, David C. Warltier, Paul S. Pagel

Biomedical Engineering Faculty Research and Publications

Background: Chronic consumption of small amounts of ethanol protects myocardium from ischemic injury. We tested the hypothesis that adenosine type 1 (A₁) receptors mediate these beneficial effects.

Methods: Dogs (n=37) were fed with ethanol (1.5 g/kg) or water mixed with dry food twice per day for 12 weeks, fasted overnight before experimentation, and instrumented for measurement of hemodynamics. Dogs received intravenous drug vehicle (50% polyethylene glycol in 0.1 N sodium hydroxide and 0.9% saline over 15 min) or the selective A₁ receptor antagonist 8-cyclopentyl-1,3-dipropylxanthine (DPCPX, 0.8 mg/kg over 15 min) and were subjected …

Strategies For Improving Neural Signal Detection Using A Neural-Electronic Interface, Robert B. Szlavik

Biomedical Engineering

There have been various theoretical and experimental studies presented in the literature that focus on interfacing neurons with discrete electronic devices, such as transistors. From both a theoretical and experimental perspective, these studies have emphasized the variability in the characteristics of the detected action potential from the nerve cell. The demonstrated lack of reproducible fidelity of the nerve cell action potential at the device junction would make it impractical to implement these devices in any neural prosthetic application where reliable detection of the action potential was a prerequisite. In this study, the effects of several different physical parameters on the …

Nonlinear Elastic Material Property Estimation Of Lower Extremity Residual Limb Tissues, Ergin TöNüK, M. Barbara Silver-Thorn

Biomedical Engineering Faculty Research and Publications

The interface stresses between the residual limb and prosthetic socket have been studied to investigate prosthetic fit. Finite-element models of the residual limb-prosthetic socket interface facilitate investigation of the mechanical interface and may serve as a potential tool for future prosthetic socket design. However, the success of such residual limb models to date has been limited, in large part due to inadequate material formulations used to approximate the mechanical behavior of residual limb soft tissues. Nonlinear finite-element analysis was used to simulate force-displacement data obtained during in vivo rate-controlled (1, 5, and 10 mm/s) cyclic indentation of the residual limb …

Measuring The Effect Of Airway Pressure On Pulmonary Arterial Diameter In The Intact Rat Lung, Robert C. Molthen, Steven Thomas Haworth, Amy Heinrich, Christopher A. Dawson

Biomedical Engineering Faculty Research and Publications

To study the relationship between transpulomnary [sic] pressure (Ptp), intravascular pressure (Pv), and the pulmonary arterial tree structure, morphometric measurements of pulmonary arterial trees were made in intact lungs from Sprague-Dawley rats. Using cone beam micro-CT and techniques we developed for imaging small animal lungs, volumetric CT data were acquired for Ptp from 0 - 12 mmHg and Pv from 5 - 30 mmHg. The diameter, D (measured range approximately 0.08-2.0 mm), vs. pressure, P, relation can be described by D(P) = D(0)(1+ α P), where α is a distensibility coefficient. Unlike studies performed in larger animals, where changes in …

Spect Imaging Of Pulmonary Blood Flow In A Rat, Christian Wieholt, Robert C. Molthen, Roger H. Johnson, Christopher A. Dawson, Anne V. Clough

Biomedical Engineering Faculty Research and Publications

Small animal imaging is experiencing rapid development due to its importance in providing high-throughput phenotypic data for functional genomics studies. We have developed a single photon emission computed tomography (SPECT) system to image the pulmonary perfusion distribution in the rat. A standard gamma camera, equipped with a pinhole collimator, was used to acquire SPECT projection images at 40 sec/view of the rat thorax following injection of Tc99m labeled albumin that accumulated in the rat's lungs. A voxel-driven, ordered-subset expectation maximization reconstruction was implemented. Following SPECT imaging, the rat was imaged using micro-CT with Feldkamp conebeam reconstruction. The two reconstructed image …

Orientation Of Self-Assembled Block Copolymer Cylinders Perpendicular To Electric Field In Mesoscale Film, S. Elhadj, J. W. Woody, V. S. Niu, Ravi F. Saraf

Ravi Saraf Publications

The possibility of using self-assembled films of block polymers as templates to fabricate nanoscale structures for devices has attracted great attention towards this class of material. Self-assembly of a block copolymer can be directed by using an electric field to orient features (i.e., ~10-nm-diam cylinders) parallel to the electric field, making the material more attractive as a nanoscale lithography mask. In this letter we describe an approach to influence the electric field orientation phenomena by interfacial effects. As a result, the 15-nm-diam polystyrene cylinders of the polystyrene–polyisoprene–polystyrene triblock copolymer orient perpendicular to the electric field. The described approach along with …