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Biomedical Engineering and Bioengineering Commons™
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Full-Text Articles in Biomedical Engineering and Bioengineering
Continuous Countercurrent Tangential Chromatography For Purification Of High Value Therapeutic Proteins, Andrew Zydney, Oleg Shinkazh, Boris Napadensky, Amit Dutta
Continuous Countercurrent Tangential Chromatography For Purification Of High Value Therapeutic Proteins, Andrew Zydney, Oleg Shinkazh, Boris Napadensky, Amit Dutta
Integrated Continuous Biomanufacturing II
One of the main challenges in the development of integrated continuous processes for the production of high value therapeutic proteins is the replacement of traditional column chromatography, which tends to dominate current downstream processing. Continuous Countercurrent Tangential Chromatography (CCTC) has been designed to provide truly continuous product capture and purification using a column-free system that overcomes many limitations of traditional column chromatography. All operations in CCTC are conducted on a moving slurry that is continuously pumped through a cascade of static mixers (for mixing and residence time) and hollow fiber membrane modules (for separation of the fluid phase from the …
Bioprocess Economics And Optimization Of Continuous And Pre-Packed Disposable Chromatography, Richard Allmendinger, Suzanne Farid
Bioprocess Economics And Optimization Of Continuous And Pre-Packed Disposable Chromatography, Richard Allmendinger, Suzanne Farid
Integrated Continuous Biomanufacturing II
The biotech sector is facing increasing pressures to design more cost-efficient, robust and flexible manufacturing processes. Standard batch chromatography (BATCH) is an established but expensive approach to separate impurities related with both E.coli and mammalian cells expression systems. This study uses a computational framework to investigate if the application of continuous chromatography (CONTI) and disposable technologies can provide a competitive alternative to BATCH and reusable equipment. A set of general assumptions is presented on how some of the key downstream processing characteristics, such as chromatography operating conditions, resin properties and equipment requirements, vary as a function of the chromatography mode …