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Full-Text Articles in Biomedical Engineering and Bioengineering
Measurement Of Red Blood Cell Oxygenation State By Magnetophoresis, Nina A. Smith
Measurement Of Red Blood Cell Oxygenation State By Magnetophoresis, Nina A. Smith
ETD Archive
Magnetophoresis of red blood cells (RBCs) at varying partial pressures of oxygen (pO2) is hypothesized to rejuvenate stored blood to be utilized beyond the FDA regulated 42-day storage time. Magnetophoresis is a particle or cells motion induced by an applied magnetic field in a viscous media. The average magnetophoretic mobility of an oxygenated RBC is -0.126x10-6 mm3-s/kg, and a deoxygenated RBC is 3.66x10-6 mm3-s/kg, presenting magnetophoresis as a resource for RBC rejuvenation in hopes of storing it longer than 42 days. The main objective of this paper was to determine if controlling the pO2 within an RBC suspension, can singly- …
High-Throughput Metabolism-Induced Toxicity Assays On A 384-Pillar Plate, Sooyeion Kang
High-Throughput Metabolism-Induced Toxicity Assays On A 384-Pillar Plate, Sooyeion Kang
ETD Archive
The U.S Environmental Protection Agency (EPA) launched the Transform Tox Testing Challenge in 2016 with the goal of developing practical methods that can be integrated into conventional high-throughput screening (HTS) assays to better predict the toxicity of parent compounds and their metabolites in vivo. In response to this need and to retrofit existing HTS assays for assessing metabolism-induced toxicity of compounds, we have developed a 384-pillar plate that is complementary to traditional 384-well plates and ideally suited for culturing human cells in three dimensions (3D) at a microscale. Briefly, human embryonic kidney (HEK) 293 cells in a mixture of alginate …
Modeling Liver Diseases Using Hepatic Cell Microarrays, Alexander David Roth
Modeling Liver Diseases Using Hepatic Cell Microarrays, Alexander David Roth
ETD Archive
Hepatocellular carcinoma (HCC) is an invasive and aggressive cancer of the liver that arises due to chronic cirrhosis. Research into understanding HCC has focused on two-dimensional (2D) and three-dimensional (3D) technologies to simulate the liver microenvironment and use animal models to model how HCC affects the rest of the body. 3D hydrogel models are desired because they can mimic the transport behavior observed in vivo by structurally mimicking the extracellular matrix (ECM) without the ethical concerns of animal models. However, hydrogels can be toxic to cells and require optimal procedures for appropriate handling. In this study, we created 3D models …
Solute Partitioning In Elastin-Like Polypeptides: A Foundation For Drug Delivery Applications, Eric Helm
Solute Partitioning In Elastin-Like Polypeptides: A Foundation For Drug Delivery Applications, Eric Helm
ETD Archive
No abstract provided.
Characterization Of Highly Concentrated Elastin-Like Polypeptide Solutions: Rheometric Properties And Phase Separation Analysis, Kevin Michael Otto
Characterization Of Highly Concentrated Elastin-Like Polypeptide Solutions: Rheometric Properties And Phase Separation Analysis, Kevin Michael Otto
ETD Archive
Elastin-like polypeptides (ELPs) are environmentally responsive biopolymers. These protein based polymers are specific in that they exhibit phase separation in response to a number of stimuli. Some of these stimuli include temperature, light, and pH. There are a large number of factors to consider when designing ELP constructs that allow for control of the transitioning behavior, some of which include amino acid sequences, protein concentration, salt concentration, and the polymer chain length. Elastin-like polypeptides are soluble in water at low temperatures, however, upon an increase in temperature, the proteins become insoluble in water and phase separate. This point of temperature …
Lipid Production By Scenedesmus Dimorphus: Comparison Of Extraction Techniques And Effect Of Sodium Nitrate Concentration, Bahareh Kanani
Lipid Production By Scenedesmus Dimorphus: Comparison Of Extraction Techniques And Effect Of Sodium Nitrate Concentration, Bahareh Kanani
ETD Archive
No abstract provided.
Moving Horizon Estimation With Dynamic Programming, Mohan Kumar Ramalingam
Moving Horizon Estimation With Dynamic Programming, Mohan Kumar Ramalingam
ETD Archive
Moving Horizon Estimation(MHE) is a optimization based strategy to state estimation. It involves computation of arrival cost, a penalty term, based on the MHE cost function. Minimization of this arrival cost is done through various methods. All these methods use nonlinear programming optimization technique which gives the estimate. The main idea of MHE revolves around minimizing the estimation cost function. The cost function is dependent on prediction error computation from data and arrival cost summarization. The major issue that hampers the MHE is choosing the arrival cost for ensuring stability of the overall estimation and computational time. In order to …