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Chemical and Biological Engineering Publications

Polyanhydride

Articles 1 - 4 of 4

Full-Text Articles in Biomedical Engineering and Bioengineering

Combinatorial Evaluation Of In Vivo Distribution Of Polyanhydride Particle-Based Platforms For Vaccine Delivery, Latrisha K. Petersen, Lucas Mark Huntimer, Katharine Walz, Amanda Ellen Ramer-Tait, Michael J. Wannemuehler, Balaji Narasimhan Jan 2013

Combinatorial Evaluation Of In Vivo Distribution Of Polyanhydride Particle-Based Platforms For Vaccine Delivery, Latrisha K. Petersen, Lucas Mark Huntimer, Katharine Walz, Amanda Ellen Ramer-Tait, Michael J. Wannemuehler, Balaji Narasimhan

Chemical and Biological Engineering Publications

Several challenges are associated with current vaccine strategies, including repeated immunizations, poor patient compliance, and limited approved routes for delivery, which may hinder induction of protective immunity. Thus, there is a need for new vaccine adjuvants capable of multi-route administration and prolonged antigen release at the site of administration by providing a depot within tissue. In this work, we designed a combinatorial platform to investigate the in vivo distribution, depot effect, and localized persistence of polyanhydride nanoparticles as a function of nanoparticle chemistry and administration route. Our observations indicated that the route of administration differentially affected tissue residence times. All ...


Harvesting Murine Alveolar Macrophages And Evaluating Cellular Activation Induced By Polyanhydride Nanoparticles., Ana Vianey Chavez-Santoscoy, Lucas Mark Huntimer, Amanda Ellen Ramer-Tait, Michael J. Wannemuehler, Balaji Narasimhan Jun 2012

Harvesting Murine Alveolar Macrophages And Evaluating Cellular Activation Induced By Polyanhydride Nanoparticles., Ana Vianey Chavez-Santoscoy, Lucas Mark Huntimer, Amanda Ellen Ramer-Tait, Michael J. Wannemuehler, Balaji Narasimhan

Chemical and Biological Engineering Publications

Biodegradable nanoparticles have emerged as a versatile platform for the design and implementation of new intranasal vaccines against respiratory infectious diseases. Specifically, polyanhydride nanoparticles composed of the aliphatic sebacic acid (SA), the aromatic 1,6-bis(p-carboxyphenoxy)hexane (CPH), or the amphiphilic 1,8-bis(p-carboxyphenoxy)-3,6-dioxaoctane (CPTEG) display unique bulk and surface erosion kinetics and can be exploited to slowly release functional biomolecules (e.g., protein antigens, immunoglobulins, etc.) in vivo. These nanoparticles also possess intrinsic adjuvant activity, making them an excellent choice for a vaccine delivery platform. In order to elucidate the mechanisms governing the activation of innate immunity ...


Design Of A Protective Single-Dose Intranasal Nanoparticle-Based Vaccine Platform For Respiratory Infectious Diseases, Bret Daniel Ulery, Devender Kumar, Amanda Ellen Ramer-Tait, Dennis W. Metzger, Michael J. Wannemuehler, Balaji Narasimhan Jan 2011

Design Of A Protective Single-Dose Intranasal Nanoparticle-Based Vaccine Platform For Respiratory Infectious Diseases, Bret Daniel Ulery, Devender Kumar, Amanda Ellen Ramer-Tait, Dennis W. Metzger, Michael J. Wannemuehler, Balaji Narasimhan

Chemical and Biological Engineering Publications

Despite the successes provided by vaccination, many challenges still exist with respect to controlling new and re-emerging infectious diseases. Innovative vaccine platforms composed of adaptable adjuvants able to appropriately modulate immune responses, induce long-lived immunity in a single dose, and deliver immunogens in a safe and stable manner via multiple routes of administration are needed. This work describes the development of a novel biodegradable polyanhydride nanoparticle-based vaccine platform administered as a single intranasal dose that induced long-lived protective immunity against respiratory disease caused by Yesinia pestis, the causative agent of pneumonic plague. Relative to the responses induced by the recombinant ...


Rational Design Of Pathogen-Mimicking Amphiphilic Materials As Nanoadjuvants, Bret Daniel Ulery, Latrisha K. Petersen, Yashdeep Phanse, Chang Sun Kong, Scott Broderick, Devender Kumar, Amanda Ellen Ramer-Tait, Brenda Rocio Carrillo-Conde, Krishna Rajan, Michael J. Wannemuehler, Bryan H. Bellaire, Dennis W. Metzger, Balaji Narasimhan Jan 2011

Rational Design Of Pathogen-Mimicking Amphiphilic Materials As Nanoadjuvants, Bret Daniel Ulery, Latrisha K. Petersen, Yashdeep Phanse, Chang Sun Kong, Scott Broderick, Devender Kumar, Amanda Ellen Ramer-Tait, Brenda Rocio Carrillo-Conde, Krishna Rajan, Michael J. Wannemuehler, Bryan H. Bellaire, Dennis W. Metzger, Balaji Narasimhan

Chemical and Biological Engineering Publications

An opportunity exists today for cross-cutting research utilizing advances in materials science, immunology, microbial pathogenesis, and computational analysis to effectively design the next generation of adjuvants and vaccines. This study integrates these advances into a bottom-up approach for the molecular design of nanoadjuvants capable of mimicking the immune response induced by a natural infection but without the toxic side effects. Biodegradable amphiphilic polyanhydrides possess the unique ability to mimic pathogens and pathogen associated molecular patterns with respect to persisting within and activating immune cells, respectively. The molecular properties responsible for the pathogen-mimicking abilities of these materials have been identified. The ...