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Biomedical Engineering and Bioengineering Commons

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Full-Text Articles in Biomedical Engineering and Bioengineering

Myosin Phosphorylation On Stress Fibers Predicts Contact Guidance Behavior Across Diverse Breast Cancer Cells, Juan Wang, Ian C. Schneider Mar 2017

Myosin Phosphorylation On Stress Fibers Predicts Contact Guidance Behavior Across Diverse Breast Cancer Cells, Juan Wang, Ian C. Schneider

Chemical and Biological Engineering Publications

During cancer progression the extracellular matrix is remodeled, forming aligned collagen fibers that proceed radially from the tumor, resulting in invasion. We have recently shown that different invasive breast cancer cells respond to epitaxially grown, aligned collagen fibrils differently. This article develops insight into why these cells differ in their contact guidance fidelity. Small changes in contractility or adhesion dramatically alter directional persistence on aligned collagen fibrils, while migration speed remains constant. The directionality of highly contractile and adhesive MDA-MB-231 cells can be diminished by inhibiting Rho kinase or β1 integrin binding. Inversely, the directionality of less contractile and adhesive ...


Cellular Contractility And Extracellular Matrix Stiffness Regulate Matrix Metalloproteinase Activity In Pancreatic Cancer Cells, Amanda Haage, Ian C. Schneider Aug 2014

Cellular Contractility And Extracellular Matrix Stiffness Regulate Matrix Metalloproteinase Activity In Pancreatic Cancer Cells, Amanda Haage, Ian C. Schneider

Chemical and Biological Engineering Publications

The pathogenesis of cancer is often driven by local invasion and metastasis. Recently, mechanical properties of the tumor microenvironment have been identified as potent regulators of invasion and metastasis, while matrix metalloproteinases (MMPs) are classically known as significant enhancers of cancer cell migration and invasion. Here we have been able to sensitively measure MMP activity changes in response to specific extracellular matrix (ECM) environments and cell contractility states. A pancreatic cancer cell line, Panc-1 cells, up-regulate MMP activities between 3- and 10- fold with increased cell contractility. Conversely, they down-regulate MMP activities when contractility is blocked to levels seen with ...


The Number Of Lines A Cell Contacts And Cell Contractility Drive The Efficiency Of Contact Guidance, Nicholas R. Romsey, Yue Hou, Laura L. Rodriguez, Ian C. Schneider Mar 2014

The Number Of Lines A Cell Contacts And Cell Contractility Drive The Efficiency Of Contact Guidance, Nicholas R. Romsey, Yue Hou, Laura L. Rodriguez, Ian C. Schneider

Chemical and Biological Engineering Publications

Cell migration is an important biological function that impacts many physiological and pathological processes. Often migration is directed along various densities of aligned fibers of collagen, a process called contact guidance. However, cells adhere to other components in the extracellular matrix, possibly affecting migrational behavior. Additionally, changes in intracellular contractility are well known to affect random migration, but its effect on contact guidance is less known. This study examines differences in directed migration in response to variations in the spacing of collagen, non-specific background adhesion strength and myosin II-mediated contractility. Collagen was microcontact printed onto glass substrates and timelapse live-cell ...


Collagen Attachment To The Substrate Controls Cell Clustering Through Migration, Yue Hou, Laura L. Rodriguez, Juan Wang, Ian C. Schneider Jan 2014

Collagen Attachment To The Substrate Controls Cell Clustering Through Migration, Yue Hou, Laura L. Rodriguez, Juan Wang, Ian C. Schneider

Chemical and Biological Engineering Publications

Cell clustering and scattering play important roles in cancer progression and tissue engineering. While the extracellular matrix (ECM) is known to control cell clustering, much of the quantitative work has focused on the analysis of clustering between cells with strong cell-cell junctions. Much less is known about how the ECM regulates cells with weak cell-cell contact. Clustering characteristics were quantified in rat adenocarcinoma cells, which form clusters on physically adsorbed collagen substrates, but not on covalently attached collagen substrates. Covalently attaching collagen inhibited desorption of collagen from the surface. While changes in proliferation rate could not explain differences seen in ...