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Biomedical Engineering and Bioengineering Commons

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Chemical and Biological Engineering Publications

Cell adhesion

2001

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Full-Text Articles in Biomedical Engineering and Bioengineering

Micropatterned Schwann Cell-Seeded Biodegradable Polymer Substrates Significantly Enhance Neurite Alignment And Outgrowth, Cheryl Miller, Srdija Jeftinija, Surya K. Mallapragada Jan 2001

Micropatterned Schwann Cell-Seeded Biodegradable Polymer Substrates Significantly Enhance Neurite Alignment And Outgrowth, Cheryl Miller, Srdija Jeftinija, Surya K. Mallapragada

Chemical and Biological Engineering Publications

Biomimetic strategies were employed to promote directional outgrowth of neurites in vitro by using a synergistic combination of physical, chemical, and cellular cues. Compression molded and solvent cast biodegradable polymer substrates made of poly(D,L-lactic acid) were micropatterned to form grooves on the substrate surfaces. Laminin was localized in the grooves, and rat sciatic Schwann cells were seeded on the substrates. Whole as well as dissociated rat dorsal root ganglia were seeded on the substrates along with Schwann cells, and neurite outgrowth and alignment were measured. The micropatterns provide physical guidance, laminin provides chemical cues, and the Schwann cells ...