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Biomedical Engineering and Bioengineering Commons

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Chemical and Biological Engineering Publications

Cell adhesion

Biological Engineering

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Full-Text Articles in Biomedical Engineering and Bioengineering

Epidermal Growth Factor-Induced Contraction Regulates Paxillin Phosphorylation To Temporally Separate Traction Generation From De-Adhesion, Ian C. Schneider, Cristen K. Hays, Clare M. Waterman Jul 2009

Epidermal Growth Factor-Induced Contraction Regulates Paxillin Phosphorylation To Temporally Separate Traction Generation From De-Adhesion, Ian C. Schneider, Cristen K. Hays, Clare M. Waterman

Chemical and Biological Engineering Publications

Directed cell migration is mediated by cycles of protrusion, adhesion, traction generation on the extracellular matrix and retraction. However, how the events after protrusion are timed, and what dictates their temporal order is completely unknown. We used acute epidermal growth factor (EGF) stimulation of epidermal keratinocytes to initiate the cell migration cycle to study the mechanism of the timing of adhesion, traction generation, and de-adhesion. Using microscopic and biochemical assays, we surprisingly found that at ∼2 min after EGF stimulation protrusion, activation of myosin-II, traction generation, adhesion assembly, and paxillin phosphorylation occurred nearly simultaneously, followed by a 10-min delay during ...