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Biomedical Engineering and Bioengineering Commons

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Chemical and Biological Engineering Publications

Cell adhesion

Articles 1 - 3 of 3

Full-Text Articles in Biomedical Engineering and Bioengineering

Epidermal Growth Factor-Induced Contraction Regulates Paxillin Phosphorylation To Temporally Separate Traction Generation From De-Adhesion, Ian C. Schneider, Cristen K. Hays, Clare M. Waterman Jul 2009

Epidermal Growth Factor-Induced Contraction Regulates Paxillin Phosphorylation To Temporally Separate Traction Generation From De-Adhesion, Ian C. Schneider, Cristen K. Hays, Clare M. Waterman

Chemical and Biological Engineering Publications

Directed cell migration is mediated by cycles of protrusion, adhesion, traction generation on the extracellular matrix and retraction. However, how the events after protrusion are timed, and what dictates their temporal order is completely unknown. We used acute epidermal growth factor (EGF) stimulation of epidermal keratinocytes to initiate the cell migration cycle to study the mechanism of the timing of adhesion, traction generation, and de-adhesion. Using microscopic and biochemical assays, we surprisingly found that at ∼2 min after EGF stimulation protrusion, activation of myosin-II, traction generation, adhesion assembly, and paxillin phosphorylation occurred nearly simultaneously, followed by a 10-min delay during ...


Synergistic Effects Of Physical And Chemical Guidance Cues On Neurite Alignment And Outgrowth On Biodegradable Polymer Substrates, Cheryl Miller, Srdija Jeftinija, Surya K. Mallapragada Jan 2002

Synergistic Effects Of Physical And Chemical Guidance Cues On Neurite Alignment And Outgrowth On Biodegradable Polymer Substrates, Cheryl Miller, Srdija Jeftinija, Surya K. Mallapragada

Chemical and Biological Engineering Publications

This article demonstrates that directional outgrowth of neurites is promoted by applying a combination of physical and chemical cues to biodegradable polymer substrates. Films of poly-D,L-lactic acid and poly(lactide-co-glycolide) were micropatterned to form grooves on substrate surfaces, using novel indirect transfer techniques developed specifically for biodegradable polymers that cannot be micropatterned directly. Laminin was selectively adsorbed in the grooves. Whole and dissociated dorsal root ganglia were seeded on the substrates and neurite outgrowth and alignment along the microgrooves were measured. The microgrooves provide physical guidance, whereas laminin provides chemical cues to the neurons. The groove depth and spacing ...


Micropatterned Schwann Cell-Seeded Biodegradable Polymer Substrates Significantly Enhance Neurite Alignment And Outgrowth, Cheryl Miller, Srdija Jeftinija, Surya K. Mallapragada Jan 2001

Micropatterned Schwann Cell-Seeded Biodegradable Polymer Substrates Significantly Enhance Neurite Alignment And Outgrowth, Cheryl Miller, Srdija Jeftinija, Surya K. Mallapragada

Chemical and Biological Engineering Publications

Biomimetic strategies were employed to promote directional outgrowth of neurites in vitro by using a synergistic combination of physical, chemical, and cellular cues. Compression molded and solvent cast biodegradable polymer substrates made of poly(D,L-lactic acid) were micropatterned to form grooves on the substrate surfaces. Laminin was localized in the grooves, and rat sciatic Schwann cells were seeded on the substrates. Whole as well as dissociated rat dorsal root ganglia were seeded on the substrates along with Schwann cells, and neurite outgrowth and alignment were measured. The micropatterns provide physical guidance, laminin provides chemical cues, and the Schwann cells ...