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Full-Text Articles in Biomedical Engineering and Bioengineering

Optimization Of Enzyme Parameters For Fermentative Production Of Biorenewable Fuels And Chemicals, Laura R. Jarboe, Ping Liu, Kumar Babu Kautharapu, Lonnie O. Ingram Oct 2012

Optimization Of Enzyme Parameters For Fermentative Production Of Biorenewable Fuels And Chemicals, Laura R. Jarboe, Ping Liu, Kumar Babu Kautharapu, Lonnie O. Ingram

Chemical and Biological Engineering Publications

Microbial biocatalysts such as Escherichia coli and Saccharomyces cerevisiae have been extensively subjected to Metabolic Engineering for the fermentative production of biorenewable fuels and chemicals. This often entails the introduction of new enzymes, deletion of unwanted enzymes and efforts to fine-tune enzyme abundance in order to attain the desired strain performance. Enzyme performance can be quantitatively described in terms of the Michaelis-Menten type parameters Km, turnover number kcat and Ki, which roughly describe the affinity of an enzyme for its substrate, the speed of a reaction and the enzyme sensitivity to inhibition by regulatory molecules. Here we describe examples of ...


Metabolic Engineering Of Biocatalysts For Carboxylic Acids Production, Ping Liu, Laura R. Jarboe Oct 2012

Metabolic Engineering Of Biocatalysts For Carboxylic Acids Production, Ping Liu, Laura R. Jarboe

Chemical and Biological Engineering Publications

Fermentation of renewable feedstocks by microbes to produce sustainable fuels and chemicals has the potential to replace petrochemical-based production. For example, carboxylic acids produced by microbial fermentation can be used to generate primary building blocks of industrial chemicals by either enzymatic or chemical catalysis. In order to achieve the titer, yield and productivity values required for economically viable processes, the carboxylic acid-producing microbes need to be robust and wellperforming. Traditional strain development methods based on mutagenesis have proven useful in the selection of desirable microbial behavior, such as robustness and carboxylic acid production. On the other hand, rationally-based metabolic engineering ...


High-Throughput Synthesis Of Carbohydrates And Functionalization Of Polyanhydride Nanoparticles, Brenda Rocio Carrillo-Conde, Rajarshi Roychoudhury, Ana Vianey Chavez-Santoscoy, Balaji Narasimhan, Nicola L.B. Pohl Jul 2012

High-Throughput Synthesis Of Carbohydrates And Functionalization Of Polyanhydride Nanoparticles, Brenda Rocio Carrillo-Conde, Rajarshi Roychoudhury, Ana Vianey Chavez-Santoscoy, Balaji Narasimhan, Nicola L.B. Pohl

Chemical and Biological Engineering Publications

Transdisciplinary approaches involving areas such as material design, nanotechnology, chemistry, and immunology have to be utilized to rationally design efficacious vaccines carriers. Nanoparticle-based platforms can prolong the persistence of vaccine antigens, which could improve vaccine immunogenicity 1. Several biodegradable polymers have been studied as vaccine delivery vehicles 1; in particular, polyanhydride particles have demonstrated the ability to provide sustained release of stable protein antigens and to activate antigen presenting cells and modulate immune responses 2-12. The molecular design of these vaccine carriers needs to integrate the rational selection of polymer properties as well as the incorporation of appropriate targeting agents ...


Dendrimer-Fullerenol Soft-Condensed Nanoassembly, Priyanka Bhattacharya, Seung Ha Kim, Pengyu Chen, Ran Chen, Anne M. Spuches, Jared M. Brown, Monica H. Lamm, Pu Chun Ke Jan 2012

Dendrimer-Fullerenol Soft-Condensed Nanoassembly, Priyanka Bhattacharya, Seung Ha Kim, Pengyu Chen, Ran Chen, Anne M. Spuches, Jared M. Brown, Monica H. Lamm, Pu Chun Ke

Chemical and Biological Engineering Publications

Nanoscale assembly is an area of research that has vast implications for molecular design, sensing, nanofabrication, supramolecular chemistry, catalysis, and environmental remediation. Here we show that poly(amidoamine) (PAMAM) dendrimers of both generations 1 (G1) and 4 (G4) can host 1 fullerenol per 2 dendrimer primary amines as evidenced by isothermal titration calorimetry, dynamic light scattering, and spectrofluorometry. Thermodynamically, the interactions were similarly spontaneous between both generations of dendrimers and fullerenols, however, G4 formed stronger complexes with fullerenols resulting from their higher surface charge density and more internal voids, as demonstrated by spectrofluorometry. In addition to hydrogen bonding that existed ...