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Biomedical Engineering and Bioengineering Commons

Open Access. Powered by Scholars. Published by Universities.®

Chemical and Biological Engineering Publications

2012

Molecular, Cellular, and Tissue Engineering

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Full-Text Articles in Biomedical Engineering and Bioengineering

Block Copolymer-Quantum Dot Micelles For Multienzyme Colocalization, Feng Jia, Yanjie Zhang, Balaji Narasimhan, Surya K. Mallapragada Jan 2012

Block Copolymer-Quantum Dot Micelles For Multienzyme Colocalization, Feng Jia, Yanjie Zhang, Balaji Narasimhan, Surya K. Mallapragada

Chemical and Biological Engineering Publications

To mimic the structure and functionality of multienzyme complexes, which are widely present in Nature, Pluronic-based micelles were designed to colocalize multiple enzymes. To stabilize the micelles as well as to enable characterization of single enzyme immobilization and multienzyme colocalization by Förster resonance energy transfer (FRET), quantum dots (QDs) were incorporated into the micelles to form Pluronic-QD micelles using a novel microreactor. Model enzymes glucose oxidase (GOX) and horseradish peroxidase (HRP) were respectively labeled with fluorescent dyes. The results indicated that FRET occurred between the QDs and dyes that labeled each type of enzyme in single enzyme immobilization studies ...