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Biomedical Engineering and Bioengineering Commons™
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Articles 1 - 7 of 7
Full-Text Articles in Biomedical Engineering and Bioengineering
Bone Marrow Cell Colonization Of, And Extracellular Matrix Expression On, Biodegradable Polymers, C. E. Holy, M. S. Shoichet, J. E. Davies
Bone Marrow Cell Colonization Of, And Extracellular Matrix Expression On, Biodegradable Polymers, C. E. Holy, M. S. Shoichet, J. E. Davies
Cells and Materials
Poly(DL-lactide-co-glycolide)s (PLGAs) have been proposed as substrata for bone tissue engineering. In the experiments reported herein, we sought to identify the optimum lactide to glycolide ratio, from the series 85:15, 75:25, 50:50, or poly-(DL-lactide) (PLA), for the elaboration of bone matrix by cultured rat bone marrow cells (RBMC) on two-dimensional substrates. Having identified PLGA 75:25 as the optimum for bone matrix elaboration by RBMC, we produced three dimensional foams from this copolymer. For the two dimensional substrata, glass coverslips were spin-coated with one of the PLGAs, or PLA. Cultures were maintained for two weeks. We employed a new technique to …
The Effect Of Vitronectin And Other Extracellular Matrix Molecules On Endothelial Expansion And Plasminogen Activation, P. Anne Underwood, Penny A. Bean
The Effect Of Vitronectin And Other Extracellular Matrix Molecules On Endothelial Expansion And Plasminogen Activation, P. Anne Underwood, Penny A. Bean
Cells and Materials
Endothelial recovery following procedures used to alleviate blood vessel occlusion is modulated by the local extracellular matrix upon which it has to migrate and proliferate. This extracellular material is derived from vessel wall cells, and plasma proteins which bind to the exposed surfaces. We have demonstrated that vitronectin adsorbs efficiently to tissue culture polystyrene in competition with other plasma proteins, which suggests that it may adsorb to biomaterial surfaces in vivo. We have compared the adhesion, migration and proliferation of human umbilical artery endothelial cells on surface-coated vitronectin, with other extracellular matrix molecules encountered in this environment, namely fibronectin, …
In Vitro Characterization Of Transforming Growth Factor-Ss1-Loaded Composites Of Biodegradable Polymer And Mesenchymal Cells, S. B. Nicoll, A. E. Denker, R. S. Tuan
In Vitro Characterization Of Transforming Growth Factor-Ss1-Loaded Composites Of Biodegradable Polymer And Mesenchymal Cells, S. B. Nicoll, A. E. Denker, R. S. Tuan
Cells and Materials
A porous, biodegradable polymer composite was fabricated from poly(L-lactic acid) (PLLA) using a solvent- casting particulate-leaching technique, and loaded with recombinant human transforming growth factor ß-1 (TGF-ß1). Tissue culture polystyrene (TCPS) and PLLA disks loaded with bovine serum albumin (BSA) served as controls. Polymer specimens were seeded at high cell density with C3H10T1/2 cells, a mouse embryonic cell line capable of differentiating into various connective tissue cell types. The polymer matrices degraded slowly in physiological saline over four weeks, with a sustained yet sporadic release of active TGF-ß1; a more controlled release of TGF-ß1 was observed in serum-containing culture medium …
Cell Adhesion To Crystal Surfaces: A Model For Initial Stages In The Attachment Of Cells To Solid Substrates, D. Hanein, B. Geiger, L. Addadi
Cell Adhesion To Crystal Surfaces: A Model For Initial Stages In The Attachment Of Cells To Solid Substrates, D. Hanein, B. Geiger, L. Addadi
Cells and Materials
This study addresses the mechanism of the chirally-restricted, ROD-independent adhesion of A6 epithelial cells to the {011} faces of calcium {R,R)-tartrate tetrahydrate crystals. The extensive and rapid adhesion of the cells to these surfaces, in the presence or absence of serum proteins, is distinctly different from the extracellular matrix-mediated adhesion to conventional tissue culture surfaces or to the {101} faces of the same crystals. The differences are manifested by insensitivity to ATP depletion, to disruption of microfilaments and microtubules and even to formaldehyde fixation of the cells. Furthermore, trypsin pretreatment does not affect cell attachment to the {011} faces, nor …
Experimental Induction Of Odontoblast Differentiation And Stimulation During Preparative Processes, H. Lesot, C. Begue-Kirn, M. D. Kubler, J. M. Meyer, A. J. Smith, N. Cassidy, J. V. Ruch
Experimental Induction Of Odontoblast Differentiation And Stimulation During Preparative Processes, H. Lesot, C. Begue-Kirn, M. D. Kubler, J. M. Meyer, A. J. Smith, N. Cassidy, J. V. Ruch
Cells and Materials
In vivo implantation experiments have shown that ethylenediaminetetraaceticacid(EDTA)-soluble frac tions of dentin stimulate reparative dentinogenesis . When isolated embryonic dental papillae were cultured in the presence of these dentin constituents, odontoblast cytological and functional differentiation could be initiated and maintained in the absence of an enamel organ. These effects were attributed to the presence of TGF-/1- related molecules [TGF-/11 or bone morphogenetic protein -2a (BMP-2a)] which had to be used in combination with an EDT A-soluble fraction of dentin in order to specifically affect competent preodontoblasts . These EDT A-soluble constituents present in dentin could be replaced by heparin or …
Comparative Assessment Of The Cytotoxicity Of Various Substrates In Organ Culture And Cell Culture: A Scanning Electron Microscopy Study, J. L. Duval, R. Warocquier-Clerout, M. F. Sigot-Luizard
Comparative Assessment Of The Cytotoxicity Of Various Substrates In Organ Culture And Cell Culture: A Scanning Electron Microscopy Study, J. L. Duval, R. Warocquier-Clerout, M. F. Sigot-Luizard
Cells and Materials
A comparative study of the behavior of chick embryo endothelial cells grown on various substrates was performed in order to establish the reliability and the limitation of both cell and organ culture methods. Following substrates were analyzed to compare these two different culture techniques: bovine serum albumin, pigskin gelatin and albumin + gelatin cross-linked by glutaraldehyde or carbodiimide, fibrin glue and negative control (Thermanox*). Parameters of cell growth and adhesion were calculated and compared with electron microscopic observations of cell morphology and of the extracellular matrix. Both culture methods provided complementary results and led to a similar classification of the …
Polylysine Stimulation Of Ectopic Cartilage Formation, Rocky S. Tuan, Dina M. Turchi, David S. Kreitzer
Polylysine Stimulation Of Ectopic Cartilage Formation, Rocky S. Tuan, Dina M. Turchi, David S. Kreitzer
Cells and Materials
Cartilage development, or chondrogenesis, is a process which involves the condensation of prechondrogenic mesenchymal cells, followed by the expression of the cartilage phenotype characterized by the biosynthesis of cartilage-specific, extracellular matrix components. We have previously shown that the cationic biopolymer, polylysine (PL), is able to stimulate chondrogenesis by embryonic limb bud mesenchymal cells in vitro. In this study, we have evaluated the chondrogenesis-stimulating activity of PL in vivo using an experimental system consisting of chick embryonic muscle explants, which were grafted onto the chorioallantoic membrane of chick embryos maintained in long-term shell-less culture. The muscle grafts were treated with PL …