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Full-Text Articles in Biomedical Engineering and Bioengineering
A Pulsatile Bioreactor For Conditioning Tissue Engineered Heart Valves, Leslie Sierad
A Pulsatile Bioreactor For Conditioning Tissue Engineered Heart Valves, Leslie Sierad
All Theses
Tissue engineered constructs with autologous adult stem cells capable of self-repair and growth are highly desired replacements for diseased heart valves. However, the current approaches have inadequate mechanical properties to withstand in vivo implantation. Therefore, our group hypothesized that an in vitro environment of physiological intra-cardiac pressures and flow will stimulate stem cells to differentiate and remodel valvular scaffold constructs before implantation.
The group developed a pneumatic-driven conditioning system (Aim I) consisting of a three-chambered heart valve bioreactor, a pressurized compliance tank, a reservoir tank, one-way valves, pressure-retaining valves, and pressure transducers. The system can be sterilized using conventional autoclaving …
Viability, Functionality, And Genetic Assessment Of Cells After Laser Guidance, Tabitha Rosenbalm
Viability, Functionality, And Genetic Assessment Of Cells After Laser Guidance, Tabitha Rosenbalm
All Theses
We systematically vary the controllable laser parameters, namely, wavelength, intensity, and exposure time of the laser on single cells to clearly establish laser parameters that allow negligible cell damage with significant cellular position control. To accomplish this goal, embryonic day 7 (E7) chick forebrains neurons were cultured with control and test cells selected one hour after seeding to allow attachment, at which time test cells were subjected to the laser. Cells were imaged at 4, 12, 24, and 36 hours after laser exposure to evaluate viability and functionality by using the Live/Dead Viability Cytotoxicity Kit (Molecular Probes L3224) and measuring …