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Interaction Of Due-B And Treslin During The Initiation Of Dna Replication, Sumeet Poudel

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The initiation of DNA replication is a highly regulated and coordinated process. To ensure that the entire genome is replicated only once per cell cycle, many replication proteins are assembled on the chromatin in a step-wise and cell cycle dependent manner. This process is controlled by interaction of replication proteins, post-translational modifications of the replication factors, control of cellular localization of the proteins, or replication factor degradation after their function terminates. Two kinases, CDK (cyclin dependent kinase) and DDK (Dbf4/Drf1 dependent kinase), play important roles during the initiation stage of DNA replication. The c-myc DNA unwinding element-binding protein (DUE-B) is …

Regulation Of The Dna Unwinding Element Binding Protein Due-B In The Cell, Yanzhe Gao

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Eukaryotic cells have evolved tightly controlled processes that make sure the entire genome gets replicated accurately, once per cell cycle. This tight control is fulfilled by degrading replication proteins after their function is carried out, changing their cellular location or by post-translational modification of replication factors to regulate their function. Phosphorylation events carried out by CDKs (cyclin dependent kinases) and DDKs (Dbf4 dependent kinases) play crucial roles in this regulation.

The DNA unwinding element binding protein (DUE-B) is an essential replication protein that binds to the human c-myc DNA replication origin. In this study, I find that DUE-B is also …

Regulation Of Dna Replication Initiation By Histone Acetylation And The Dna Unwinding Element Binding Protein Due-B, Michael George Kemp

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Duplication of the genome during S phase of the mitotic cell cycle begins at thousands of sites along chromosomes termed origins of replication. Although many of the essential protein components catalyzing events at these sites are known and are conserved throughout eukaryotes, the likelihood or efficiency of initiation of DNA synthesis at any given genomic site is expected to be influenced by other novel factors, including aspects of chromatin and DNA structure.

Here I show that increased histone H4 acetylation at replication origin loci occurs after treatment with the histone deacetylase inhibitor TSA and coincides with a loss of specific …