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Biomedical Engineering and Bioengineering Commons™
Open Access. Powered by Scholars. Published by Universities.®
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- Metabolic pathways (2)
- A549 cells (1)
- Algae (1)
- Antibiotic resistance (1)
- Antimicrobial delivery vehicle (1)
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- Biofilms (1)
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- Breast (1)
- Carbon nanotubes (1)
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- E. Coli (1)
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- Predictive mathematical models (1)
- Protein conformational imprinting (1)
- Singlewalled carbon nanotube (1)
- Surgery (1)
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- Thermoreversible gel (1)
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Articles 1 - 8 of 8
Full-Text Articles in Biomedical Engineering and Bioengineering
Designing Predictive Mathematical Models For The Metabolic Pathways Associated With Polyhydroxybutyrate Synthesis In Escherichia Coli, Angela Dixon
All Graduate Theses and Dissertations, Spring 1920 to Summer 2023
Plastics are a versatile and widely used material. However, traditional plastics are derived from petrochemicals and are not biodegradable. Polymers synthesized from microorganisms that have similar properties to plastic are potential biodegradable replacements. The objective of this project is to use mathematical modeling as a tool to engineer a strain of bacteria optimized for the production of bio-plastics.
Production costs can be reduced by using a bacterial strain specifically optimized for bio-plastic production. By reducing production costs, bio-plastics will be able to commercially compete with traditional plastics. Society will benefit as bio-plastics replace traditional plastics. Fossil fuels will not be …
Predictive Mathematical Model For Polyhydroxybutyrate Synthesis In Escherichia Coli, Angela Dixon
Predictive Mathematical Model For Polyhydroxybutyrate Synthesis In Escherichia Coli, Angela Dixon
Engineering Datasets
Polyhydroxybutyrate has been studied as a potential biodegradable replacement for petrochemical plastics. Polyhydroxybutyrate synthesis is not native to Escherichia coli, but the genes have successfully been inserted through plasmids. However, polyhydroxybutyrate production needs to be more cost-effective before it can be commercially produced. A mathematical model for polyhydroxybutyrate synthesis was developed to identify genes that could be altered to increase polyhydroxybutyrate production. The major metabolic pathways included in the model were glycolysis, acetyl coenzyme-A synthesis, tricarboxylic acid cycle, glyoxylate bypass, and polyhydroxybutyrate synthesis. Reactions were modeled using kinetic mechanisms identified for each enzyme. The transcriptional network was incorporated into the …
Looking Below The Surface Of Breast Tissue During Surgery, A Mahadevan-Jansen, Md Keller, Elizabeth Vargis, B Caldwell, T-Q Nguyen, Ndm Granja, M Sanders, Mc Kelley
Looking Below The Surface Of Breast Tissue During Surgery, A Mahadevan-Jansen, Md Keller, Elizabeth Vargis, B Caldwell, T-Q Nguyen, Ndm Granja, M Sanders, Mc Kelley
Biological Engineering Faculty Publications
In this article, we present a method that provides prompt detection of the presence of cancer cells inside the 2-mm margin of tissue surrounding the tumor after excision using spatially offset Raman spectroscopy (SORS). SORS was developed to detect subtle changes in soft tissue spectra in the 100–2000 μm range and tested on excised breast tissues. The results display a very high specificity and sensitivity (100% and 95%, respectively) of classification between positive and negative tumor margins. SORS is a clinically feasible method, suitable for the real-time, intraoperative assessment of tumor margins at the micrometer level.
Characterization And Application Of Dynamic In Vitro Models Of Human Airway, Hemangkumar J. Patel
Characterization And Application Of Dynamic In Vitro Models Of Human Airway, Hemangkumar J. Patel
All Graduate Theses and Dissertations, Spring 1920 to Summer 2023
In recent years, respiratory diseases have emerged as a leading cause of mortality across the globe. In the United States alone respiratory diseases are the fourth leading cause of deaths annually. Moreover, with the rapid increase of industrialization and urbanization, the occurrences of respiratory diseases are expected to remain high with strong chances of increasing in the future. To ameliorate the epidemic of respiratory disease, it is first important to understand its underlying mechanisms.
Respiratory research studies in animals have elucidated the chronological order of the pathological events and systemic responses inside the lung, but understanding the response of individual …
Combating Antibiotic Resistance Through Development Of A Novel Antimicrobial Delivery Vehicle, Alex Hatch
Combating Antibiotic Resistance Through Development Of A Novel Antimicrobial Delivery Vehicle, Alex Hatch
Undergraduate Honors Capstone Projects
A thermoreversible gel for antimicrobial agent delivery would provide a novel platform suitable for medical applications from persistent infections arising from localized biofilms. Thermoreversible gels could be used to coat medical implants, to inject for subdermal treatments, or to implement in topical applications. FDA approved Pluronics® form thermoreversible gels and certain Pluronics® display inherent varying degrees of antimicrobial activity. Pluronics® have been shown to affect efflux pumps, and reduce ATP levels within the bacterial cell. In combination with antibiotics and other pharmaceuticals, Pluronics® have been used to sequester drugs and act as a delivery vehicle. With advances in nanomedicine, nanoparticles …
Fluoro-Silane As A Functional Monomer For Protein Conformational Imprinting, Yun Peng
Fluoro-Silane As A Functional Monomer For Protein Conformational Imprinting, Yun Peng
All Graduate Theses and Dissertations, Spring 1920 to Summer 2023
By using the technology of molecularly imprinted polymer (MIP), we propose to synthesize a protein conformational imprint that also acts as a plastic enzyme, inducing protein structural transitions. The imprint aims at MIP-induced stabilization and / or formation of bound protein secondary structure and the applications associated with analysis and correction of misfolded proteins. The screening of polymeric functional monomers being able to induce the conformational transitions in proteins is investigated in this report.
The fluoro-silanes (3-heptafluoroisopropoxy)propalethoxysilane (7F) and 3,3,3-trifluoropropylmethoxysilane (3F) were employed as functional monomers for synthesis of this catalytic protein conformational imprint via sol-gel reactions. 3F was demonstrated …
A Study On The Applications And Toxicity Assessments Of Carbon Nanotubes In Tissue Engineering, Rena Baktur
A Study On The Applications And Toxicity Assessments Of Carbon Nanotubes In Tissue Engineering, Rena Baktur
All Graduate Theses and Dissertations, Spring 1920 to Summer 2023
Carbon nanotubes (CNTs) are one of the most popular nanomaterials. There has been increasing interest in the development and applications of carbon nanotubes due to their huge potential in industrial and medical applications. Recent applications of carbon nanotubes include development of scaffolds and drug delivery systems. Despite rapidly emerging applications of CNTs, little is known about the impact of CNTs on cellular processes, especially mesenchymal stem cell (MSC)'s differentiation. Also, the effects of nanoparticle exposure under different conditions on cellular responses have not been well characterized yet.
To characterize the effects of CNTs on creating nanoscale scaffolds for tissue engineering, …
Algal Biofilm Production And Harvesting System For Wastewater Treatment With Biofuels By-Products, Logan Christenson
Algal Biofilm Production And Harvesting System For Wastewater Treatment With Biofuels By-Products, Logan Christenson
All Graduate Theses and Dissertations, Spring 1920 to Summer 2023
Excess nitrogen and phosphorus in discharged wastewaters can lead to downstream eutrophication, ecosystem damage, and impaired water quality that may affect human health. Chemical-based and physical-based technologies are available to remove these nutrients; however, they often consume significant amounts of energy and chemicals, greatly increasing treatment costs. Algae are capable of removing these pollutants through biomass assimilation, and if harvested, can be utilized as a feedstock for biomethane or biodiesel production. Currently, difficulties in harvesting, concentrating, and dewatering algae have limited the development of an economically feasible treatment and production process. When algae are grown as surface-attached biofilms, the biomass …