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Full-Text Articles in Biomedical Engineering and Bioengineering
Characterization Of Human Muscle Extracellular Matrix, Lauren Klaire Wilson
Characterization Of Human Muscle Extracellular Matrix, Lauren Klaire Wilson
Graduate Theses and Dissertations
The performance of extracellular matrix (ECM) biological scaffolds for the treatment of volumetric muscle loss (VML) has shown promising results with regenerating native muscle in animal models and human patients. However, the limitations of these scaffolds include non-specific characteristics based on the original parameters of the muscle that is lost and this is why our group chose to characterize human skeletal ECM. By understanding the characteristics of the native human skeletal ECM, more desired queues for the biological scaffolds being used to treat VML can be provided. Upper limb and lower limb skeletal muscles were commercially obtained, decellularized using common …
Development Of A Novel Device For The Perfusion Driven Decellularization Of Skeletal Muscle, Benjamin Kasukonis
Development Of A Novel Device For The Perfusion Driven Decellularization Of Skeletal Muscle, Benjamin Kasukonis
Graduate Theses and Dissertations
Decellularization of skeletal muscle is a process that removes cellular components of skeletal muscle tissue while leaving behind the intact extracellular matrix (ECM). Skeletal muscle ECM is currently being studied as a biologic scaffold for repairing volumetric muscle loss (VML) because the removal of cells greatly reduces the antigenicity of the donor tissue. Decellularization usually relies on passive diffusion of detergents, surfactants and/or osmotic solutions to strip cells from the ECM. However, passive diffusion alone is usually not sufficient for complete removal of cells from the interior of large pieces of skeletal muscle using detergents, such as sodium dodecyl sulfate …