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Full-Text Articles in Biomedical Engineering and Bioengineering
Fgf2-Induced Effects On Transcriptome Associated With Regeneration Competence In Adult Human Fibroblasts, Olga Kashpur, David Lapointe, Sakthikumar Ambady, Elizabeth Ryder, Tanja Dominko
Fgf2-Induced Effects On Transcriptome Associated With Regeneration Competence In Adult Human Fibroblasts, Olga Kashpur, David Lapointe, Sakthikumar Ambady, Elizabeth Ryder, Tanja Dominko
Sakthikumar Ambady
BACKGROUND: Adult human fibroblasts grown in low oxygen and with FGF2 supplementation have the capacity to tip the healing outcome of skeletal muscle injury - by favoring regeneration response in vivo over scar formation. Here, we compare the transcriptomes of control adult human dermal fibroblasts and induced regeneration-competent (iRC) fibroblasts to identify transcriptional changes that may be related to their regeneration competence. RESULTS: We identified a unique gene-expression profile that characterizes FGF2-induced iRC fibroblast phenotype. Significantly differentially expressed genes due to FGF2 treatment were identified and analyzed to determine overrepresented Gene Ontology terms. Genes belonging to extracellular matrix components, adhesion …
Expression And Differentiation Between Oct4a And Its Pseudogenes In Human Escs And Differentiated Adult Somatic Cells., Sakthikumar Ambady, Mojca Jez, Olga Kashpur, Alexandra Grella, Christopher Malcuit, Lucy Vilner, Primoz Rozman, Tanja Dominko
Expression And Differentiation Between Oct4a And Its Pseudogenes In Human Escs And Differentiated Adult Somatic Cells., Sakthikumar Ambady, Mojca Jez, Olga Kashpur, Alexandra Grella, Christopher Malcuit, Lucy Vilner, Primoz Rozman, Tanja Dominko
Sakthikumar Ambady
The POU5F1 gene codes for the OCT4 transcription factor, which is one of the key regulators of pluripotency. Its transcription, alternative splicing, and alternative translation leading to the synthesis of the active, nuclear localized OCT4A has been described in detail. Much less, however, is known about actively transcribed OCT4 pseudogenes, several of which display high homology to OCT4A and can be expressed and translated into proteins. Using RT-PCR followed by pseudogene-specific restriction digestion, cloning, and sequencing we discriminate between OCT4A and transcripts for pseudogenes 1, 3 and 4. We show that expression of OCT4 and its pseudogenes follows a developmentally-regulated …
Fgf2-Induced Effects On Transcriptome Associated With Regeneration Competence In Adult Human Fibroblasts, Sakthikumar Ambady, Olga Kashpur, David Lapointe, Elizabeth Ryder, Tanja Dominko
Fgf2-Induced Effects On Transcriptome Associated With Regeneration Competence In Adult Human Fibroblasts, Sakthikumar Ambady, Olga Kashpur, David Lapointe, Elizabeth Ryder, Tanja Dominko
Sakthikumar Ambady
Adult human fibroblasts grown in low oxygen and with FGF2 supplementation have the capacity to tip the healing outcome of skeletal muscle injury - by favoring regeneration response in vivo over scar formation. Here, we compare the transcriptomes of control adult human dermal fibroblasts and induced regeneration-competent (iRC) fibroblasts to identify transcriptional changes that may be related to their regeneration competence. RESULTS: We identified a unique gene-expression profile that characterizes FGF2-induced iRC fibroblast phenotype. Significantly differentially expressed genes due to FGF2 treatment were identified and analyzed to determine overrepresented Gene Ontology terms. Genes belonging to extracellular matrix components, adhesion molecules, …
Alterations In P53 And E2f-1 Function Common To Immortalized Chicken Embryo Fibroblasts, Sakthikumar Ambady, Hyunggee Kim, Seungkwon You, In-Jeong Kim, Linda Foster, James Farris, F Able Ponce Leon, Douglas Foster
Alterations In P53 And E2f-1 Function Common To Immortalized Chicken Embryo Fibroblasts, Sakthikumar Ambady, Hyunggee Kim, Seungkwon You, In-Jeong Kim, Linda Foster, James Farris, F Able Ponce Leon, Douglas Foster
Sakthikumar Ambady
A number of non-virally and non-chemically immortalized chicken embryo fibroblast (CEF) cells have been established recently in continuous cell culture. All immortal CEF cells tested showed common genetic alterations in the expression patterns of p53 and E2F-1 mRNA and protein which were down- and up-regulated, respectively. The biological effects of differentially regulated p53 and E2F-1 were determined by reporter gene transcriptional activity assays, DNA binding assays, and Northern blot analysis of the expression patterns of down-stream genes. In addition, expression of most of the cyclin genes was up-regulated in immortal CEF cells, which may be associated with the rapid cell …
Development Of A Bovine X Chromosome Linkage Group And Painting Probes To Assess Cattle, Sheep, And Goat X Chromosome Segment Homologies., Sakthikumar Ambady, F.Abel Ponce Leon, Gregory Hawkins, Steven Kappes, Michael Bishop, James Robl, Craig Beattle
Development Of A Bovine X Chromosome Linkage Group And Painting Probes To Assess Cattle, Sheep, And Goat X Chromosome Segment Homologies., Sakthikumar Ambady, F.Abel Ponce Leon, Gregory Hawkins, Steven Kappes, Michael Bishop, James Robl, Craig Beattle
Sakthikumar Ambady
The X chromosome linkage group is conserved in placental mammals. However, X chromosome morphological differences, due to internal chromosome rearrangements, exist among mammalian species. We have developed bovine chromosome painting probes for Xp and Xq to assess segment homologies between the submetacentric bovine X chromosome and the acrocentric sheep and goat X chromosomes. These painting probes and their corresponding DNA libraries were developed by chromosome micromanipulation, DNA micropurification, microcloning, and PCR amplification. The bovine Xp painting probe identified an interstitially located homologous segment in the sheep and goat Xq region, most probably resulting from chromosome inversion. Ten type II (microsatellite) …