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Full-Text Articles in Biomedical Engineering and Bioengineering

Neuron-Glial (Ng) Interactions: A Microfluidic Examination Of Ng Emergent Responses For Repair, Tanya Singh Jan 2019

Neuron-Glial (Ng) Interactions: A Microfluidic Examination Of Ng Emergent Responses For Repair, Tanya Singh

Dissertations and Theses

Neuron-glia communication is crucial to the development, plasticity, and repair of the nervous system (NS). While neurons are well known to conduct electrical impulses that transfer biological information and stimuli throughout the NS, our understanding of the roles of glia continues to evolve from when the cells were largely believed to act solely for neuronal support. Recent decades of research has shown that glia can alter metabolism, conduct impulses and change phenotype for NS repair. NG interactions have, thereby, become heavily researched in varied areas of biomedical engineering, including embryogenesis, neural regeneration, growth, and intracellular synaptic activity. However, while NG ...


A Drosophila Model To Examine Collective Migration During Retinogenesis, Caroline Pena, Stephanie Zhang, Mildred Kamara, Tadmiri Venkatesh, Maribel Vazquez Apr 2017

A Drosophila Model To Examine Collective Migration During Retinogenesis, Caroline Pena, Stephanie Zhang, Mildred Kamara, Tadmiri Venkatesh, Maribel Vazquez

Publications and Research

Retinal dysfunction is often caused by aberrant neural cell migration during development. In this study, we observed the migration of neural cells of the Drosophila melanogaster after marking cells of the 3rd instar larvae with the GAL4-UAS expression system when exposed to a concentration gradient of FGF-8 through the use of a microfluidic device. The glial and neuronal cell ratio in the developing brain was determined through immunofluorescent staining and observation. In future studies, a microfluidic device that mimics the developing Drosophila brain and retina will be designed in order to better understand the biological factors that affect the migration ...


Controlled Microfluidics To Examine Growth-Factor Induced Migration Of Neural Progenitors In The Drosophila Visual System, Cade Beck, Tanya Singh, Angela Farooqi, Tadmiri Venkatesh, Maribel Vazquez Mar 2016

Controlled Microfluidics To Examine Growth-Factor Induced Migration Of Neural Progenitors In The Drosophila Visual System, Cade Beck, Tanya Singh, Angela Farooqi, Tadmiri Venkatesh, Maribel Vazquez

Publications and Research

BACKGROUND:

The developing visual system in Drosophila melanogaster provides an excellent model with which to examine the effects of changing microenvironments on neural cell migration via microfluidics, because the combined experimental system enables direct genetic manipulation, in vivo observation, and in vitro imaging of cells, post-embryo. Exogenous signaling from ligands such as fibroblast growth factor (FGF) is well-known to control glia differentiation, cell migration, and axonal wrapping central to vision.

NEW METHOD:

The current study employs a microfluidic device to examine how controlled concentration gradient fields of FGF are able to regulate the migration of vision-critical glia cells with and ...


Chemotaxis Of Drosophila Glia With Controlled Microenvironments, Cade Beck Jan 2012

Chemotaxis Of Drosophila Glia With Controlled Microenvironments, Cade Beck

Dissertations and Theses

No abstract provided.


Low Concentration Microenvironments Enhance The Migration Of Neonatal Cells Of Glial Lineage, Richard A. Able, Jr., Celestin Ngnabeuye, Cade Beck, Eric C. Holland, Maribel Vazquez Jun 2002

Low Concentration Microenvironments Enhance The Migration Of Neonatal Cells Of Glial Lineage, Richard A. Able, Jr., Celestin Ngnabeuye, Cade Beck, Eric C. Holland, Maribel Vazquez

Publications and Research

Glial tumors have demonstrated abilities to sustain growth via recruitment of glial progenitor cells (GPCs), which is believed to be driven by chemotactic cues. Previous studies have illustrated that mouse GPCs of different genetic backgrounds are able to replicate the dispersion pattern seen in the human disease. How GPCs with genetic backgrounds transformed by tumor paracrine signaling respond to extracellular cues via migration is largely unexplored, and remains a limiting factor in utilizing GPCs as therapeutic targets. In this study, we utilized a microfluidic device to examine the chemotaxis of three genetically-altered mouse GPC populations towards tumor conditioned media, as ...