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Full-Text Articles in Biomedical Engineering and Bioengineering

Composite Biomaterial Repair Strategy To Restore Biomechanical Function And Reduce Herniation Risk In An Ex Vivo Large Animal Model Of Intervertebral Disc Herniation With Varying Injury Severity, Warren W. Hom, Melanie Tschopp, Huizi A. Lin, Philip Nasser, Damien M. Laudier, Andrew C. Hecht, Steven B. Nicoll, James C. Iatridis May 2019

Composite Biomaterial Repair Strategy To Restore Biomechanical Function And Reduce Herniation Risk In An Ex Vivo Large Animal Model Of Intervertebral Disc Herniation With Varying Injury Severity, Warren W. Hom, Melanie Tschopp, Huizi A. Lin, Philip Nasser, Damien M. Laudier, Andrew C. Hecht, Steven B. Nicoll, James C. Iatridis

Publications and Research

Back pain commonly arises from intervertebral disc (IVD) damage including annulus fibrosus (AF) defects and nucleus pulposus (NP) loss. Poor IVD healing motivates developing tissue engineering repair strategies. This study evaluated a composite injectable IVD biomaterial repair strategy using carboxymethylcellulose-methylcellulose (CMC-MC) and genipincrosslinked fibrin (FibGen) that mimic NP and AF properties, respectively. Bovine ex vivo caudal IVDs were evaluated in cyclic compression-tension, torsion, and compression-to-failure tests to determine IVD biomechanical properties, height loss, and herniation risk following experimentally-induced severe herniation injury and discectomy (4 mm biopsy defect with 20% NP removed). FibGen with and without CMC-MC had failure strength similar ...


The Effect Of Heterobifunctional Crosslinkers On Hema Hydrogel Modulus And Toughness, Elizabeth M. Boazak, Vaughn K. Greene Jr., Debra T. Auguste May 2019

The Effect Of Heterobifunctional Crosslinkers On Hema Hydrogel Modulus And Toughness, Elizabeth M. Boazak, Vaughn K. Greene Jr., Debra T. Auguste

Publications and Research

The use of hydrogels in load bearing applications is often limited by insufficient toughness. 2-Hydroxyethyl methacrylate (HEMA) based hydrogels are appealing for translational work, as they are affordable and the use of HEMA is FDA approved. Furthermore, HEMA is photopolymerizable, providing spatiotemporal control over mechanical properties. We evaluated the ability of vinyl methacrylate (VM), allyl methacrylate (AM), and 3-(Acryloyloxy)-2-hydroxypropyl methacrylate (AHPM) to tune hydrogel toughness and Young’s modulus. The crosslinkers were selected due to their heterobifunctionality (vinyl and methacrylate) and similar size and structure to EGDMA, which was shown previously to increase toughness as compared to longer ...


The Modeling, Design, Fabrication, And Application Of Biosensor Based On Electric Cell-Substrate Impedance Sensing (Ecis) Technique In Environmental Monitoring, Xudong Zhang, William Wang, Sunghoon Jang Apr 2019

The Modeling, Design, Fabrication, And Application Of Biosensor Based On Electric Cell-Substrate Impedance Sensing (Ecis) Technique In Environmental Monitoring, Xudong Zhang, William Wang, Sunghoon Jang

Publications and Research

In this research, the modeling, design, fabrication, and application of ECIS sensors in environmental monitoringare studied. The ECIS sensors are able to qualify the water toxicity through measuring the cell impedance. A novel mathematical model is proposed to analyze the distribution of electric potential and current of ECIS. This mathematical model is validated by experimental data and can be used to optimize the dimension of ECIS electrodes in order to satisfy environmental monitors. The detection sensitivity of ECIS sensors is analyzed by the mathematical model and experimental data. The simulated and experimental results show that ECIS sensors with smaller radius ...


Bme 20500 Bioelectrical Circuits, Jacek P. Dmochowski, Luis Cardoso Jan 2019

Bme 20500 Bioelectrical Circuits, Jacek P. Dmochowski, Luis Cardoso

Open Educational Resources

No abstract provided.


Bioelectrical Circuits: Lecture 9, Jacek P. Dmochowski, Luis Cardoso Jan 2019

Bioelectrical Circuits: Lecture 9, Jacek P. Dmochowski, Luis Cardoso

Open Educational Resources

No abstract provided.


Bioelectrical Circuits: Lecture 10, Jacek P. Dmochowski, Luis Cardoso Jan 2019

Bioelectrical Circuits: Lecture 10, Jacek P. Dmochowski, Luis Cardoso

Open Educational Resources

No abstract provided.


Bioelectrical Circuits: Lecture 7, Jacek P. Dmochowski, Luis Cardoso Jan 2019

Bioelectrical Circuits: Lecture 7, Jacek P. Dmochowski, Luis Cardoso

Open Educational Resources

No abstract provided.


Bioelectrical Circuits: Lecture 1, Jacek P. Dmochowski, Luis Cardoso Jan 2019

Bioelectrical Circuits: Lecture 1, Jacek P. Dmochowski, Luis Cardoso

Open Educational Resources

No abstract provided.


Bioelectrical Circuits: Lecture 3, Jacek P. Dmochowski, Luis Cardoso Jan 2019

Bioelectrical Circuits: Lecture 3, Jacek P. Dmochowski, Luis Cardoso

Open Educational Resources

No abstract provided.


Bioelectrical Circuits: Lecture 4, Jacek P. Dmochowski, Luis Cardoso Jan 2019

Bioelectrical Circuits: Lecture 4, Jacek P. Dmochowski, Luis Cardoso

Open Educational Resources

No abstract provided.


Bioelectrical Circuits: Lecture 5, Jacek P. Dmochowski, Luis Cardoso Jan 2019

Bioelectrical Circuits: Lecture 5, Jacek P. Dmochowski, Luis Cardoso

Open Educational Resources

No abstract provided.


Bioelectrical Circuits: Lecture 8, Jacek P. Dmochowski, Luis Cardoso Jan 2019

Bioelectrical Circuits: Lecture 8, Jacek P. Dmochowski, Luis Cardoso

Open Educational Resources

No abstract provided.


Bioelectrical Circuits: Lecture 6, Jacek P. Dmochowski, Luis Cardoso Jan 2019

Bioelectrical Circuits: Lecture 6, Jacek P. Dmochowski, Luis Cardoso

Open Educational Resources

No abstract provided.


Bioelectrical Circuits: Lecture 2, Jacek P. Dmochowski, Luis Cardoso Jan 2019

Bioelectrical Circuits: Lecture 2, Jacek P. Dmochowski, Luis Cardoso

Open Educational Resources

No abstract provided.


Msg-Fast: Metagenomic Shotgun Data Fast Annotation Using Microbial Gene Catalogs, Stuart M. Brown, Hao Chen, Yuhan Hao, Bobby P. Laungani, Thahmina A. Ali, Changsu Dong, Carlos Lijeron, Baekdoo Kim, Claudia Wultsch, Zhiheng Pei, Konstantinos Krampis Jan 2019

Msg-Fast: Metagenomic Shotgun Data Fast Annotation Using Microbial Gene Catalogs, Stuart M. Brown, Hao Chen, Yuhan Hao, Bobby P. Laungani, Thahmina A. Ali, Changsu Dong, Carlos Lijeron, Baekdoo Kim, Claudia Wultsch, Zhiheng Pei, Konstantinos Krampis

Publications and Research

Background: Current methods used for annotating metagenomics shotgun sequencing (MGS) data rely on a computationally intensive and low-stringency approach of mapping each read to a generic database of proteins or reference microbial genomes.

Results: We developed MGS-Fast, an analysis approach for shotgun whole-genome metagenomic data utilizing Bowtie2 DNA-DNA alignment of reads that is an alternative to using the integrated catalog of reference genes database of well-annotated genes compiled from human microbiome data. This method is rapid and provides high-stringency matches (>90% DNA sequence identity) of the metagenomics reads to genes with annotated functions. We demonstrate the use of this method ...


Translational Modeling Of Non-Invasive Electrical Stimulation, Dennis Quangvinh Truong Jan 2019

Translational Modeling Of Non-Invasive Electrical Stimulation, Dennis Quangvinh Truong

Dissertations and Theses

Seminal work in the early 2000’s demonstrated the effect of low amplitude non-invasive electrical stimulation in people using neurophysiological measures (motor evoked potentials, MEPs). Clinical applications of transcranial Direct Current Stimulation (tDCS) have since proliferated, though the mechanisms are not fully understood. Efforts to refine the technique to improve results are on-going as are mechanistic studies both in vivo and in vitro. Volume conduction models are being applied to these areas of research, especially in the design and analysis of clinical montages. However, additional research on the parameterization of models remains.

In this dissertation, Finite Element Method (FEM) models ...


Neuron-Glial (Ng) Interactions: A Microfluidic Examination Of Ng Emergent Responses For Repair, Tanya Singh Jan 2019

Neuron-Glial (Ng) Interactions: A Microfluidic Examination Of Ng Emergent Responses For Repair, Tanya Singh

Dissertations and Theses

Neuron-glia communication is crucial to the development, plasticity, and repair of the nervous system (NS). While neurons are well known to conduct electrical impulses that transfer biological information and stimuli throughout the NS, our understanding of the roles of glia continues to evolve from when the cells were largely believed to act solely for neuronal support. Recent decades of research has shown that glia can alter metabolism, conduct impulses and change phenotype for NS repair. NG interactions have, thereby, become heavily researched in varied areas of biomedical engineering, including embryogenesis, neural regeneration, growth, and intracellular synaptic activity. However, while NG ...


Modification Of Lipid Microenvironments On Solid Support Structures For Use In Transmembrane Protein Assays, William J. Houlihan Jan 2019

Modification Of Lipid Microenvironments On Solid Support Structures For Use In Transmembrane Protein Assays, William J. Houlihan

Dissertations and Theses

Gamma-Secretase (γ-secretase) is a transmembrane protease of increasing interest, which has been shown to have significant connections to both cancer and Alzheimer’s disease. γ-secretase cleaves both Notch-1, a transmembrane signaling protein, and Amyloid precursor protein (APP), a transmembrane protein whose cleavage may result in the formation of β-amyloid plaques in the brain. Notch-1 and APP are widely studied proteins that have substantial impacts on the development and proliferation of cancer and Alzheimer’s disease, respectively. Notch-1 partakes in the signaling of apoptosis in damaged and mutated cells, thus its cleavage by γ-secretase within the plasma membrane has ramifications on ...


Controlled Migration Of Retinal Progenitor Cells Within Electro-Chemotactic Fields, Shawn Mishra Jan 2019

Controlled Migration Of Retinal Progenitor Cells Within Electro-Chemotactic Fields, Shawn Mishra

Dissertations and Theses

Vision loss in retinal degenerative diseases is overwhelmingly attributed to damage and death of retinal photoreceptor cells. Studies in mouse retina have suggested that transplantation of isolated post-natal or stem cell-derived retinal progenitor cells (RPCs) to replace apoptotic or damaged photoreceptors may be a novel approach to restore vision. Thus far, outcomes project that the amount of restored visual response depends upon the migration of transplanted cells from insertion in the sub-retinal space to the outer nuclear layer (ONL). However, transplantation efficiency is exceedingly low – ~5% cells transplanted enter the retina – directly limiting the efficacy of the treatments. Additionally, the ...