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Biomedical Engineering and Bioengineering Commons™
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- Discipline
- Keyword
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- Adherens junction; Adhesion strengthening; Actin; VE-cadherin; Ena/VASP (1)
- Bioengineering (1)
- Capillary morphogenesis - Endothelial cell - Biomaterial - Self assembly - Mechanical properties - Adhesion (1)
- Capillary morphogenesis;endothelial cell;biomaterial;self-assembling;adhesion (1)
- Cell adhesion (1)
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- Class (1)
- Education (1)
- Endothelial cells (1)
- In vitro angiogenesis; Cell traction; Capillary morphogenesis; Matrix stiffness (1)
- Microarchitecture (1)
- Microvasculature (1)
- Tissue engineering (1)
- Tissue engineering; Micropatterning; Cellular alignment; 3D engineered tissue (1)
- Tissue engineering; chondrocyte; growth factor; mixing; bioreactors (1)
- Transport (1)
Articles 1 - 10 of 10
Full-Text Articles in Biomedical Engineering and Bioengineering
Developing A Small-Footprint Bioengineering Program, Alisha Sarang-Sieminski, Debbie Chachra
Developing A Small-Footprint Bioengineering Program, Alisha Sarang-Sieminski, Debbie Chachra
Alisha L. Sarang-Sieminski
The field of bioengineering is rapidly changing and expanding to include not only more traditional bioengineering applications (e.g. device-focused areas such as prosthetics, imaging) but also more recent sub-fields and technologies(e.g. more biologically-focused areas such as those enabled by tissue engineering and microfluidics). This rapid change, coupled with the intrinsically interdisciplinary nature of bioengineering, presents a unique challenge to the developers of academic programs, as they need to both select relevant content and strike a balance between depth and breadth. We, the architects of the bioengineering program at the undergraduate-only Franklin W. Olin College of Engineering, which enrolled its first …
Directed 3d Cell Alignment And Elongation In Microengineered Hydrogels, Alisha Sarang-Sieminski, Hug Aubin, Jason Nichol, Ché Hutson, Hojae Bae, Donald Cropek, Payam Akhyari, Ali Khademhosseini
Directed 3d Cell Alignment And Elongation In Microengineered Hydrogels, Alisha Sarang-Sieminski, Hug Aubin, Jason Nichol, Ché Hutson, Hojae Bae, Donald Cropek, Payam Akhyari, Ali Khademhosseini
Alisha L. Sarang-Sieminski
Organized cellular alignment is critical to controlling tissue microarchitecture and biological function. Although a multitude of techniques have been described to control cellular alignment in 2D, recapitulating the cellular alignment of highly organized native tissues in 3D engineered tissues remains a challenge. While cellular alignment in engineered tissues can be induced through the use of external physical stimuli, there are few simple techniques for microscale control of cell behavior that are largely cell-driven. In this study we present a simple and direct method to control the alignment and elongation of fibroblasts, myoblasts, endothelial cells and cardiac stem cells encapsulated in …
Igf-I And Mechanical Environment Interact To Modulate Engineered Cartilage Development, Keith Gooch, Torsten Blunk, D. Courter, Alisha Sarang-Sieminski, Predrag Bursac, Gordana Vunjak-Novakovic, Lisa Freed
Igf-I And Mechanical Environment Interact To Modulate Engineered Cartilage Development, Keith Gooch, Torsten Blunk, D. Courter, Alisha Sarang-Sieminski, Predrag Bursac, Gordana Vunjak-Novakovic, Lisa Freed
Alisha L. Sarang-Sieminski
Bovine calf articular chondrocytes were seeded onto biodegradable polyglycolic acid scaffolds and cultured for four weeks using in vitro systems providing different mechanical environments (static and mixed Petri dishes, static and mixed flasks, and rotating vessels) and different biochemical environments (medium with and without supplemental insulin-like growth factor I, IGF-I). Under all conditions, the resulting engineered tissue histologically resembled cartilage and contained its major constituents: glycosaminoglycans, collagen, and cells. The mechanical environment and supplemental IGF-I (a) independently modulated tissue morphology, growth, biochemical composition, and mechanical properties (equilibrium modulus) of engineered cartilage as previously reported; (b) interacted additively or in some …
Engineering Strategies To Recapitulate Epithelial Morphogenesis Within Synthetic Three-Dimensional Extracellular Matrix With Tunable Mechanical Properties, Alisha Sarang-Sieminski, Yekaterina Miroshnikova, D Jorgens, L Spirio, M Auer, V Weaver
Engineering Strategies To Recapitulate Epithelial Morphogenesis Within Synthetic Three-Dimensional Extracellular Matrix With Tunable Mechanical Properties, Alisha Sarang-Sieminski, Yekaterina Miroshnikova, D Jorgens, L Spirio, M Auer, V Weaver
Alisha L. Sarang-Sieminski
The mechanical properties (e.g. stiffness) of the extracellular matrix (ECM) influence cell fate and tissue morphogenesis and contribute to disease progression. Nevertheless, our understanding of the mechanisms by which ECM rigidity modulates cell behavior and fate remains rudimentary. To address this issue, a number of two and three-dimensional (3D) hydrogel systems have been used to explore the effects of the mechanical properties of the ECM on cell behavior. Unfortunately, many of these systems have limited application because fiber architecture, adhesiveness and/or pore size often change in parallel when gel elasticity is varied. Here we describe the use of ECM-adsorbed, synthetic, …
Self-Assembling Short Oligopeptides And The Promotion Of Angiogenesis, Alisha Sarang-Sieminski, Daria Narmoneva, Olumuyiwa Oni, Shugang Zhang, Jonathan Gertler, Roger Kamm, Richard Lee
Self-Assembling Short Oligopeptides And The Promotion Of Angiogenesis, Alisha Sarang-Sieminski, Daria Narmoneva, Olumuyiwa Oni, Shugang Zhang, Jonathan Gertler, Roger Kamm, Richard Lee
Alisha L. Sarang-Sieminski
Because an adequate blood supply to and within tissues is an essential factor for successful tissue regeneration, promoting a functional microvasculature is a crucial factor for biomaterials. In this study, we demonstrate that short self-assembling peptides form scaffolds that provide an angiogenic environment promoting long-term cell survival and capillary-like network formation in three-dimensional cultures of human microvascular endothelial cells. Our data show that, in contrast to collagen type I, the peptide scaffold inhibits endothelial cell apoptosis in the absence of added angiogenic factors, accompanied by enhanced gene expression of the angiogenic factor VEGF. In addition, our results suggest that the …
Primary Sequence Of Ionic Self-Assembling Peptide Gels Affects Endothelial Cell Adhesion And Capillary Morphogenesis, Alisha Sarang-Sieminski, C. Semino, Haiyan Gong, Roger Kamm
Primary Sequence Of Ionic Self-Assembling Peptide Gels Affects Endothelial Cell Adhesion And Capillary Morphogenesis, Alisha Sarang-Sieminski, C. Semino, Haiyan Gong, Roger Kamm
Alisha L. Sarang-Sieminski
Appropriate choice of biomaterial supports is critical for the study of capillary morphogenesis in vitro as well as to support vascularization of engineered tissues in vivo. Self-assembling peptides are a class of synthetic, ionic, oligopeptides that spontaneously assemble into gels with an ECM-like microarchitecture when exposed to salt. In this paper, the ability of four different self-assembling peptide gels to promote endothelial cell adhesion and capillary morphogenesis is explored. Human umbilical vein endothelial cells (HUVECs) were cultured within ionic self-assembling peptide family members, RAD16-I ((RADA)4), RAD16-II ((RARADADA)2), KFE-8 ((FKFE)2), or KLD-12 ((KLDL)3). …
Biomaterial-Microvasculature Interactions, Alisha Sarang-Sieminski, Keith Gooch
Biomaterial-Microvasculature Interactions, Alisha Sarang-Sieminski, Keith Gooch
Alisha L. Sarang-Sieminski
The utility of implanted sensors, drug-delivery systems, immunoisolation devices, engineered cells, and engineered tissues can be limited by inadequate transport to and from the circulation. As the primary function of the microvasculature is to facilitate transport between the circulation and the surrounding tissue, interactionsbetween biomaterials and the microvasculature have been explored to understand the mechanisms controlling transport to implanted objects and ultimately improve it. This review surveys work on biomaterial-microvasculature interactions with a focus on the use of biomaterials to regulate the structure and function of the microvasculature. Several applications in which biomaterial-microvasculature interactions play a crucial role are briefly …
The Relative Magnitudes Of Endothelial Force Generation And Matrix Stiffness Modulate Capillary Morphogenesis In Vitro, Alisha Sarang-Sieminski, Robert Hebbel, Keith Gooch
The Relative Magnitudes Of Endothelial Force Generation And Matrix Stiffness Modulate Capillary Morphogenesis In Vitro, Alisha Sarang-Sieminski, Robert Hebbel, Keith Gooch
Alisha L. Sarang-Sieminski
When suspended in collagen gels, endothelial cells elongate and form capillary-like networks containing lumens. Human blood outgrowth endothelial cells (HBOEC) suspended in relatively rigid 3 mg/ml floating collagen gels, formed in vivo-like, thin, branched multi-cellular structures with small, thick-walled lumens, while human umbilical vein endothelial cells (HUVEC) formed fewer multi-cellular structures, had a spread appearance, and had larger lumens. HBOEC exert more traction on collagen gels than HUVEC as evidenced by greater contraction of floating gels. When the stiffness of floating gels was decreased by decreasing the collagen concentration from 3 to 1.5 mg/ml, HUVEC contracted gels more and formed …
The Stiffness Of Three-Dimensional Ionic Self-Assembling Peptide Gels Affects The Extent Of Capillary-Like Network Formation, Alisha Sarang-Sieminski, A Was, G Kim, Haiyan Gong, Roger Kamm
The Stiffness Of Three-Dimensional Ionic Self-Assembling Peptide Gels Affects The Extent Of Capillary-Like Network Formation, Alisha Sarang-Sieminski, A Was, G Kim, Haiyan Gong, Roger Kamm
Alisha L. Sarang-Sieminski
Improving our ability to control capillary morphogenesis has implications for not only better understanding of basic biology, but also for applications in tissue engineering and in vitro testing. Numerous biomaterials have been investigated as cellular supports for these applications and the biophysical environment biomaterials provide to cells has been increasingly recognized as an important factor in directing cell function. Here, the ability of ionic self-assembling peptide gels to support capillary morphogenesis and the effect of their mechanical properties is investigated. When placed in a physiological salt solution, these oligopeptides spontaneously self-assemble into gels with an extracellular matrix (ECM)-like microarchitecture. To …
Vasp Involvement In Force-Mediated Adherens Junction Strengthening, Alisha Sarang-Sieminski, Anita Kris, Roger Kamm
Vasp Involvement In Force-Mediated Adherens Junction Strengthening, Alisha Sarang-Sieminski, Anita Kris, Roger Kamm
Alisha L. Sarang-Sieminski
Strengthening of cell-matrix adhesions in response to applied force has been well documented. However, while implied by various lines of evidence, the force-mediated strengthening of cell–cell adhesions has not been directly demonstrated. In the current study, we present results consistent with force strengthening in adherens junctions, obtained by application of different force profiles to VE-cadherin-coated magnetic beads attached to endothelial cells. When force is ramped from a low to high value over time, fewer beads detach than with the immediate application of high force. Cells treated with cytochalasin D or lacking Ena/VASP activity show similar levels of detachment relative to …