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Biomedical Engineering and Bioengineering Commons

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Full-Text Articles in Biomedical Engineering and Bioengineering

Modeling Alveolar Epithelial Cell Behavior In Spatially Designed Hydrogel Microenvironments, Katherine Jean Reeder Lewis Jan 2016

Modeling Alveolar Epithelial Cell Behavior In Spatially Designed Hydrogel Microenvironments, Katherine Jean Reeder Lewis

Chemical & Biological Engineering Graduate Theses & Dissertations

The alveolar epithelium consists of two cell phenotypes, elongated alveolar type I cells (AT1) and rounded alveolar type II cells (ATII), and exists in a complex three-dimensional environment as a polarized cell layer attached to a thin basement membrane and enclosing a roughly spherical lumen. Closely surrounding the alveolar cysts are capillary endothelial cells as well as interstitial pulmonary fibroblasts. Many factors are thought to influence alveolar epithelial cell differentiation during lung development and wound repair, including physical and biochemical signals from the extracellular matrix (ECM), and paracrine signals from the surrounding mesenchyme. In particular, disrupted signaling between the alveolar ...


Process Extension From Embryonic Stem Cell-Derived Motor Neurons Through Synthetic Extracellular Matrix Mimics, Daniel Devaud Mckinnon Jan 2014

Process Extension From Embryonic Stem Cell-Derived Motor Neurons Through Synthetic Extracellular Matrix Mimics, Daniel Devaud Mckinnon

Chemical & Biological Engineering Graduate Theses & Dissertations

This thesis focuses on studying the extension of motor axons through synthetic poly(ethylene glycol) PEG hydrogels that have been modified with biochemical functionalities to render them more biologically relevant. Specifically, the research strategy is to encapsulate embryonic stem cell-derived motor neurons (ESMNs) in synthetic PEG hydrogels crosslinked through three different chemistries providing three mechanisms for dynamically tuning material properties. First, a covalently crosslinked, enzymatically degradable hydrogel is developed and exploited to study the biophysical dynamics of axon extension and matrix remodeling. It is demonstrated that dispersed motor neurons require a battery of adhesive peptides and growth factors to maintain ...


Fundamental Characterization Of Photodegradable Hydrogels: Spatiotemporal Control Of The Cellular Microenvironment, Mark William Tibbitt Jan 2012

Fundamental Characterization Of Photodegradable Hydrogels: Spatiotemporal Control Of The Cellular Microenvironment, Mark William Tibbitt

Chemical & Biological Engineering Graduate Theses & Dissertations

Synthetic hydrogels are an attractive class of materials for the design of well-defined cell culture platforms to better understand how cells receive and integrate signals present in the extracellular environment. Within this class, responsive hydrogels have emerged to investigate questions as to how cells sense and respond to dynamic changes in the mechanical and biochemical nature of the cell niche. To complement this growing body of research, this thesis aimed to develop photoresponsive, synthetic hydrogels that enable experimenters to explore how spatiotemporally varying cues from the extracellular matric influence biological function in real time. Specifically, cytocompatible, chain and step polymerized ...