Biomedical Engineering and Bioengineering Commons^™

Using Collagen Binding Poly(N-Isopropylacrylamide) Nanoparticles To Prevent Intravascular Platelet Adhesion And Activation, Anna E. Searle, Alyssa Panitch, James Mcmasters

The Summer Undergraduate Research Fellowship (SURF) Symposium

Balloon angioplasty, the most prevalent non-surgical treatment for Atherosclerosis, damages the endothelial layer of the artery, baring an underlying collagenous layer, which causes platelet adhesion and activation and eventual thrombosis and intimal hyperplasia. Previous work in our lab has used a collagen-binding peptidoglycan, dermatan-sulfate-SILY (DS-SILY), that has been shown to bind to type I collagen and prevent platelet adhesion and activation. Our goal is to fabricate nanoparticle-SILY by cross-linking SILY to a poly(N-isopropylacrylamide) (NIPAm) nanoparticle instead of a DS backbone, while retaining the SILY’s high collagen binding affinity and platelet inhibition capacity observed in DS-SILY. Using a biotin-streptavidin assay, we …