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Biomedical Engineering and Bioengineering Commons

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Full-Text Articles in Biomedical Engineering and Bioengineering

Alginate Hydrogels As Three-Dimensional Scaffolds For In Vitro Culture Models Of Growth Plate Cartilage Development And Porcine Embryo Elongation, Taylor D. Laughlin Jul 2016

Alginate Hydrogels As Three-Dimensional Scaffolds For In Vitro Culture Models Of Growth Plate Cartilage Development And Porcine Embryo Elongation, Taylor D. Laughlin

Biological Systems Engineering--Dissertations, Theses, and Student Research

The establishment of in vitro culture models utilizes tissue engineering principles to design functional mimics of in vivo environments in vitro. Advantages for the use of in vitro culture models include ethical alleviation of animal models for therapeutic testing, cost efficiency, and a greater ability to study specific mechanisms via a systematic, ground-up approach to development. In this thesis, alginate hydrogels are utilized in the development of in vitro culture models of porcine embryo elongation and growth plate cartilage development. First, the effect of scaffold and modifications to the scaffold were explored in both projects. In order to modulate cell-scaffold ...


Enzymatically Degradable Poly(Ethylene Glycol) Hydrogels For Diverse Tissue Engineering Applications And For The Study Of The Foreign Body Response, Luke Daniel Amer Jan 2016

Enzymatically Degradable Poly(Ethylene Glycol) Hydrogels For Diverse Tissue Engineering Applications And For The Study Of The Foreign Body Response, Luke Daniel Amer

Chemical Engineering Graduate Theses & Dissertations

The field of tissue engineering aims to create replacements for diseased or damaged tissues which restore function and integrate with the host. Poly(ethylene glycol) (PEG) hydrogels are promising materials for the delivery of cells for tissue engineering applications, however, as with all synthetic materials, they elicit a foreign body response (FBR) upon implantation. Generally, the FBR begins with an initial inflammatory response and ends with the encapsulation of the implant in an avascular fibrous capsule. The harsh environment present during the FBR may harm encapsulated cells and impair integration into the host, ultimately reducing the efficacy of tissue engineering ...


Use Of Biofunctional Hydrogel Matrices For Chondrocyte Transplantation Applications, Balaji V. Sridhar Jan 2015

Use Of Biofunctional Hydrogel Matrices For Chondrocyte Transplantation Applications, Balaji V. Sridhar

Chemical & Biological Engineering Graduate Theses & Dissertations

Healing joint articular cartilage is a significant clinical challenge because it lacks self-healing properties. Focal defects that do not heal properly tend to progress to debilitating osteoarthritis that affects millions of people worldwide. Tissue engineering strategies that utilize biofunctional scaffolds as chondrocyte carriers present a promising treatment option to regenerate cartilage tissue. Autologous chondrocytes are a good cell source since they regulate cartilage extracellular matrix (ECM) production in the tissue. Cells can be combined with photopolymerizable scaffolds, which permit control over network formation and can be modified to present biological cues. Current treatment options for cartilage regeneration have generally yielded ...


Humidity Effect On The Structure Of Electrospun Core-Shell Pcl-Peg Fibers For Tissue Regeneration Applications, Adam P. Golin Apr 2014

Humidity Effect On The Structure Of Electrospun Core-Shell Pcl-Peg Fibers For Tissue Regeneration Applications, Adam P. Golin

Electronic Thesis and Dissertation Repository

With the aim of creating a biodegradable scaffold for tympanic membrane (TM) tissue regeneration, core-shell nanofibers composed of a poly(caprolactone) shell and a poly(ethylene glycol) core were created using a coaxial electrospinning technique. In order to create fibers with an optimal core-shell morphology, the effect of relative humidity (RH) on the core-shell nanofibers was systematically studied, with a FITC-BSA complex encapsulated in the core to act as a model protein. The core-shell nanofibers were electrospun at relative humidity values of 20, 25, 30, and 40% RH within a glove box outfitted for humidity control. The core-shell morphology of ...


Poly(Ethylene Glycol) Based Biomaterial Platforms For Guiding Cell Behavior Through Control Of Presentation And Release Of Bioactive, Therapeutic Proteins., Joshua Daniel Mccall Jan 2012

Poly(Ethylene Glycol) Based Biomaterial Platforms For Guiding Cell Behavior Through Control Of Presentation And Release Of Bioactive, Therapeutic Proteins., Joshua Daniel Mccall

Chemical & Biological Engineering Graduate Theses & Dissertations

Poly(ethylene glycol) (PEG) based biomaterials offer a number of advantages for applications in biomedical technology, including drug delivery and tissue engineering. PEG is notable for both its hydrophilicity and bioinert properties, and is widely used to create hydrogels used for 3D cell culture. There is growing interest in strategies to introduce biological functionality into PEG-based materials, in order to develop platforms to study the complex biochemical and biomechanical cues that govern cell behavior in physiologically relevant context. This thesis explores photopolymerization conditions for hydrogel synthesis that maintain a high degree of bioactivity for proteins in-situ. Such reactions are then ...


Fundamental Characterization Of Photodegradable Hydrogels: Spatiotemporal Control Of The Cellular Microenvironment, Mark William Tibbitt Jan 2012

Fundamental Characterization Of Photodegradable Hydrogels: Spatiotemporal Control Of The Cellular Microenvironment, Mark William Tibbitt

Chemical & Biological Engineering Graduate Theses & Dissertations

Synthetic hydrogels are an attractive class of materials for the design of well-defined cell culture platforms to better understand how cells receive and integrate signals present in the extracellular environment. Within this class, responsive hydrogels have emerged to investigate questions as to how cells sense and respond to dynamic changes in the mechanical and biochemical nature of the cell niche. To complement this growing body of research, this thesis aimed to develop photoresponsive, synthetic hydrogels that enable experimenters to explore how spatiotemporally varying cues from the extracellular matric influence biological function in real time. Specifically, cytocompatible, chain and step polymerized ...


Topography, Extracellular Matrix Proteins, Secreted Molecules And Endogenous Electric Fields: Cues That Influence The Differentiation Of Neural Progenitor Cells, Carlos Atico Ariza Jan 2009

Topography, Extracellular Matrix Proteins, Secreted Molecules And Endogenous Electric Fields: Cues That Influence The Differentiation Of Neural Progenitor Cells, Carlos Atico Ariza

Graduate Theses and Dissertations

Neural progenitor cells (NPCs) have the potential to be used as a cell based therapy to treat Alzheimer disease, spinal cord injury and other significant damage to the central nervous system. In order to utilize the therapeutic potential of NPCs, we must first learn to control their migration, proliferation, differentiation and growth. An ideal methodology would entail directed stem cell migration to damaged tissue; proliferation until the target is reached; differentiation into the most beneficial phenotype; and integration of cells into the existing tissue. A myriad of stimulants that alter NPC behavior, exist in vivo. Characterizing the individual contributions of ...