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Biomedical Engineering and Bioengineering Commons

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Chemical Engineering

Western University

Quantum dots

Publication Year

Articles 1 - 2 of 2

Full-Text Articles in Biomedical Engineering and Bioengineering

Nanostructured Biosensor For Tear Glucose Detection Based On Bioluminescence Resonance Energy Transfer (Bret) Mechanism., Denghuang Zhan Aug 2017

Nanostructured Biosensor For Tear Glucose Detection Based On Bioluminescence Resonance Energy Transfer (Bret) Mechanism., Denghuang Zhan

Electronic Thesis and Dissertation Repository

Bioluminescence Resonance Energy Transfer (BRET), a sensitive, non-destructive and self-illuminated method, has been now commonly used to test protein interactions. Here, we describe a BRET sensor for non-invasively detecting glucose molecules. The sensor is made by the bioconjugation of quantum dots and recombinant protein. The recombinant protein contains the bacterial glucose binding protein (GBP) and a bioluminescent protein, Renilla luciferase (Rluc), used as the donor with the emission peak at 470 nm, which is able to excite the acceptor of BRET sensor made of cadmium tellurium quantum dots ( CdTe QDs) with the emission peak at 570 nm. The distance between ...


Surface Functionalization And Bioconjugation Of Nanoparticles For Biomedical Applications, Longyan Chen Jan 2014

Surface Functionalization And Bioconjugation Of Nanoparticles For Biomedical Applications, Longyan Chen

Electronic Thesis and Dissertation Repository

Colloidal inorganic nanoparticles (NPs) have been attracting considerable interest in biomedicine, from drug and gene delivery to imaging, sensing and diagnostics. It is essential to modify the surface of nanoparticles to have enhanced biocompatibility and functionality for the in vitro and in vivo applications, especially in delivering locally and recognizing biomolecules. Herein, the goal of this research work is to develop advanced NPs with well-tailored surface functionalities and/or bio-functionality for the applications in cell tracking and analytes detection.

In the first project, quantum dots incorporating with gelatin nanoparticles (QDs-GNPs) have been developed for bioimaging applications. Two different approaches have ...