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Biomedical Engineering and Bioengineering Commons

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Chemical Engineering

University of Massachusetts Medical School

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Full-Text Articles in Biomedical Engineering and Bioengineering

Polymeric Nanoparticles For Targeted Combination Treatment Of Temozolomide Resistant Glioblastoma Multiforme (Gbm), Praveena Velpurisiva, Brandon Piel, Jack Lepine, Prakash Rai May 2017

Polymeric Nanoparticles For Targeted Combination Treatment Of Temozolomide Resistant Glioblastoma Multiforme (Gbm), Praveena Velpurisiva, Brandon Piel, Jack Lepine, Prakash Rai

UMass Center for Clinical and Translational Science Research Retreat

Glioblastoma Multiforme (GBM) is an aggressive cancer that originates from astrocytes and spreads to spinal cord and other parts of the brain. Increase in replication of glial cells leads to advantageous mutations in the tumor. According to the cancer statistics from 2015 about 15,320 deaths were reported due to GBM. Five-year survival is less than 5% making GBM a dreadful form of cancer. Current treatment involves complex invasive surgery, followed by chemotherapy and radiation. The goal of this study is to develop a combination therapy to treat GBM using Poly (lactic-co-glycolic acid) (PLGA) nanoparticles encapsulated with two drugs namely ...


Building Better Tumor Models In Vitro: An Investigation Into The Improvement Of 3d Cell Culture Techniques, Brandon Piel, Michael Doane, Praveena Velpurisiva, Prakash Rai May 2017

Building Better Tumor Models In Vitro: An Investigation Into The Improvement Of 3d Cell Culture Techniques, Brandon Piel, Michael Doane, Praveena Velpurisiva, Prakash Rai

UMass Center for Clinical and Translational Science Research Retreat

In cancer drug discovery, 3D cell culture is a segue between monolayer cell culture and animal testing, offering better predictive modelling of drug performance before animal testing commences. However, even though cell spheroids in 3D cultures superficially resemble tumors, they typically lack the complexity and scale of tumors formed in vivo. Spheroids typically consist of a single cell type whereas tumors contain a whole ecosystem of cells. Also, most 3D protocols stop at day 10, where the spheroids are roughly 500-600 μm in diameter at the largest, whereas tumors that develop in the body are, on average, 7.5 cm ...


Getting To The Root Of Bacterial Hairs: What Is “S”?, Rebecca Gaddis, Samantha O'Conner, Evan Anderson, Terri Camesano, Nancy Burnham May 2014

Getting To The Root Of Bacterial Hairs: What Is “S”?, Rebecca Gaddis, Samantha O'Conner, Evan Anderson, Terri Camesano, Nancy Burnham

UMass Center for Clinical and Translational Science Research Retreat

An atomic force microscope (AFM) was used to measure the steric forces of lipopolysaccharides (LPS) on the biofilm-forming bacteria, Pseudomonas aeruginosa. It is well known that LPS play a vital role in biofilm formation. These forces were characterized with a modified version of the Alexander and de Gennes (AdG) model for polymers, which is a function of equilibrium brush length, L, probe radius, R, temperature, T, separation distance, D, and an indefinite density variable, s. This last parameter was originally distinguished by de Gennes as the root spacing or mesh spacing depending upon the type of polymer adhesion; however since ...